superscript ii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript ii
    Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii/product/Thermo Fisher
    Average 99 stars, based on 3396 article reviews
    Price from $9.99 to $1999.99
    superscript ii - by Bioz Stars, 2020-07
    99/100 stars

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    Irradiation:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    RNA Extraction:

    Article Title: Annexin A1 down-regulation in head and neck squamous cell carcinoma is mediated via transcriptional control with direct involvement of miR-196a/b
    Article Snippet: .. RNA extraction and real-time RT-PCR Total RNA was extracted from HNSCC cells using Trizol reagent (Invitrogen Life Technologies), and cDNA synthesized with Superscript II RT-PCR System (Invitrogen Life Technologies), according to manufacturer’s protocols. .. Gene expression was analyzed by Real-time PCR using the StepOnePlus Real-Time PCR System (Applied Biosystems) following Applied Biosystems’ SYBR Green Master Mix protocol.

    Amplification:

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Clone Assay:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    Synthesized:

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
    Article Snippet: .. Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer. .. About 6 kb long L1Rn sequences encompassing ORF1 and ORF2 were amplified from cDNA with the Taq/Pwo Proof Mix (AGS-Hybaid) and primers Pr-L1Rn1 (5′-GGA AGA GAC CAC CAA CAC TGC TCA C-3′, pos.

    Article Title: Annexin A1 down-regulation in head and neck squamous cell carcinoma is mediated via transcriptional control with direct involvement of miR-196a/b
    Article Snippet: .. RNA extraction and real-time RT-PCR Total RNA was extracted from HNSCC cells using Trizol reagent (Invitrogen Life Technologies), and cDNA synthesized with Superscript II RT-PCR System (Invitrogen Life Technologies), according to manufacturer’s protocols. .. Gene expression was analyzed by Real-time PCR using the StepOnePlus Real-Time PCR System (Applied Biosystems) following Applied Biosystems’ SYBR Green Master Mix protocol.

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Quantitative RT-PCR:

    Article Title: Annexin A1 down-regulation in head and neck squamous cell carcinoma is mediated via transcriptional control with direct involvement of miR-196a/b
    Article Snippet: .. RNA extraction and real-time RT-PCR Total RNA was extracted from HNSCC cells using Trizol reagent (Invitrogen Life Technologies), and cDNA synthesized with Superscript II RT-PCR System (Invitrogen Life Technologies), according to manufacturer’s protocols. .. Gene expression was analyzed by Real-time PCR using the StepOnePlus Real-Time PCR System (Applied Biosystems) following Applied Biosystems’ SYBR Green Master Mix protocol.

    Purification:

    Article Title: Developmental arrest of T cells in RpL22-deficient mice is dependent upon multiple p53 effectors 1
    Article Snippet: .. Purified mRNA was converted to cDNA using Superscript II reverse transcription PCR with oligo dT(12–18) primers (Invitrogen, Carlsbad, CA). .. Quantitative real-time PCR was performed on the Prism 7700 thermocycler (Applied Biosystems, Carlsbad, CA) using Taqman Real-time PCR primer/probe sets specific to murine PUMA/ Bbc3 (Mm00519268_m1), Noxa/ Pmaip1 (Mm00451763_m1), and Bim/( Bcl2l-11 ) (Mm01333921_m1).

    Real-time Polymerase Chain Reaction:

    Article Title: Sex-Differences in Renal Expression of Selected Transporters and Transcription Factors in Lean and Obese Zucker Spontaneously Hypertensive Fatty Rats
    Article Snippet: .. 2.4. cDNA Synthesis and TaqMan Real-Time PCR Superscript II Reverse Transcriptase (Life Technologies, Darmstadt, Germany) and Oligo dT-Primers (Eurofin MWG Operon, Ebersberg, Germany) were used for reverse transcription of RNA. .. Genes of interest were analyzed using TaqMan Master Mix and TaqMan Gene Expression Assays (Life Technologies): Sodium-dependent glucose cotransporter 1 (Sglt1), Rn00564718_m1; Sglt2, Rn00574917_m1; Oat1, Rn00568143_m1; Oat2, Rn00585513_m1; Oat3, Rn00580082_m1; Oct1, Rn00562250_m1; Oct2, Rn00580893_m1; Mrp2, Rn00563231_m1; Mrp4, Rn01465702_m1; P-glycoprotein (Mdr1b), Rn00561753_m1; Hnf1α , Rn00562020_m1; Hnf1β , Rn00447453_m1; Hnf4α , Rn00573309_m1.

    Article Title: Receptor Tyrosine Kinase-like Orphan Receptor 2 (Ror2) Expression Creates a Poised State of Wnt Signaling in Renal Cancer *
    Article Snippet: .. Total RNA was extracted from cells using a Qiagen RNeasy mini kit (Valencia, CA). cDNA was made from 500 ng of total RNA using random primers (Invitrogen) and Superscript II RT-PCR reagents (Invitrogen) and analyzed using the ABI 7900HT fast real-time PCR system with the following proprietary FAM-labeled primers: Ror2, Axin2, Fzd1, Jun, 18S, and β-actin (Applied Biosystems, Foster City, CA). .. Wnt-related gene expression was examined with the human Wnt signaling RT2 Profiler PCR array (SABiosciences, Frederick, MD) using cDNA from 786-0 +TAP-hRor2, 786-0, and 786-0 shRor2.2 cells with the ABI 7500 real-time PCR system.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Annexin A1 down-regulation in head and neck squamous cell carcinoma is mediated via transcriptional control with direct involvement of miR-196a/b
    Article Snippet: .. RNA extraction and real-time RT-PCR Total RNA was extracted from HNSCC cells using Trizol reagent (Invitrogen Life Technologies), and cDNA synthesized with Superscript II RT-PCR System (Invitrogen Life Technologies), according to manufacturer’s protocols. .. Gene expression was analyzed by Real-time PCR using the StepOnePlus Real-Time PCR System (Applied Biosystems) following Applied Biosystems’ SYBR Green Master Mix protocol.

    Article Title: Receptor Tyrosine Kinase-like Orphan Receptor 2 (Ror2) Expression Creates a Poised State of Wnt Signaling in Renal Cancer *
    Article Snippet: .. Total RNA was extracted from cells using a Qiagen RNeasy mini kit (Valencia, CA). cDNA was made from 500 ng of total RNA using random primers (Invitrogen) and Superscript II RT-PCR reagents (Invitrogen) and analyzed using the ABI 7900HT fast real-time PCR system with the following proprietary FAM-labeled primers: Ror2, Axin2, Fzd1, Jun, 18S, and β-actin (Applied Biosystems, Foster City, CA). .. Wnt-related gene expression was examined with the human Wnt signaling RT2 Profiler PCR array (SABiosciences, Frederick, MD) using cDNA from 786-0 +TAP-hRor2, 786-0, and 786-0 shRor2.2 cells with the ABI 7500 real-time PCR system.

    Polymerase Chain Reaction:

    Article Title: Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues
    Article Snippet: .. RNA samples were treated with RQ1 RNase-Free DNase (Promega, Madison, WI). cDNA was synthesized from 2 μg of total RNA using random hexamers and Superscript II RNase H- reverse transcriptase (Invitrogen®, Carlsbad, CA), according to the manufacturer’s recommendations followed by PCR amplification using specific primers for the RVT and a region between Integrase and the chromodomain of Corky (Figure ). .. As expected, amplification products were not obtained in RNA samples not yielded to reverse transcription prior to PCR. cDNA was stored at −20°C.

    Article Title: Developmental arrest of T cells in RpL22-deficient mice is dependent upon multiple p53 effectors 1
    Article Snippet: .. Purified mRNA was converted to cDNA using Superscript II reverse transcription PCR with oligo dT(12–18) primers (Invitrogen, Carlsbad, CA). .. Quantitative real-time PCR was performed on the Prism 7700 thermocycler (Applied Biosystems, Carlsbad, CA) using Taqman Real-time PCR primer/probe sets specific to murine PUMA/ Bbc3 (Mm00519268_m1), Noxa/ Pmaip1 (Mm00451763_m1), and Bim/( Bcl2l-11 ) (Mm01333921_m1).

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    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 4652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase/product/Thermo Fisher
    Average 95 stars, based on 4652 article reviews
    Price from $9.99 to $1999.99
    superscript ii reverse transcriptase - by Bioz Stars, 2020-07
    95/100 stars
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    94
    Thermo Fisher superscript ii kit
    Superscript Ii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii kit/product/Thermo Fisher
    Average 94 stars, based on 295 article reviews
    Price from $9.99 to $1999.99
    superscript ii kit - by Bioz Stars, 2020-07
    94/100 stars
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    93
    Thermo Fisher superscript ii rtpcr enzyme
    Superscript Ii Rtpcr Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii rtpcr enzyme/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superscript ii rtpcr enzyme - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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