superscript ii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript ii
    Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii/product/Thermo Fisher
    Average 99 stars, based on 1284 article reviews
    Price from $9.99 to $1999.99
    superscript ii - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ). .. For NoV, a hemi-nested RT-PCR protocol was used, targeting a highly conserved region in the diagnostic region C (ORF2) located on the capsid protein VP1, with minor modifications (Kojima et al. ; Nishida et al. ).

    Amplification:

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: Genomic DNA was removed by digestion with Amplification Grade DNase I (Sigma-Aldrich). .. First-strand cDNA was synthesized by reverse transcription of 1 μg of total RNA using Superscript-II and random primers (Invitrogen) according to standard protocols.

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA). .. For the LCAT splicing experiments, 2 μl of the reverse transcription reaction was subjected to 20 rounds of PCR amplification in a 20 μl reaction (1:10 dilution) using LCAT specific primers spiked with a kinased LCAT forward primer (0.4 nM).

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: A 200-bp fragment of viral RNA was amplified using the Hot Star Taq Master mix kit (Qiagen, Milan, Italy). .. First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA). .. End-repair, 3′-adenylation, ligation of indexed adaptors and PCR amplification was performed according to Illumina protocols.

    DNA Synthesis:

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA). .. End-repair, 3′-adenylation, ligation of indexed adaptors and PCR amplification was performed according to Illumina protocols.

    Synthesized:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: .. PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: .. First-strand cDNA was synthesized by reverse transcription of 1 μg of total RNA using Superscript-II and random primers (Invitrogen) according to standard protocols. .. Quantitative real-time PCR Relative expression levels of WNK1-4 mRNA were determined by real time RT-PCR using Absolute SYBR Green Mix (ABgene, Epsom, UK) and the following set of primers: WNK1-for (5′-AAGGTCTGGACACCGAAACC-3′), WNK1-rev (5′-TTCCCTTTTACTGTGGATTCCC-3′), WNK2-for (5′-CATGACATGGAGGCCTCTGG-3′), WNK2-rev (5′-CGGGCTTTTCACTCTCAGGA-3′), WNK3-for (5′-CATCACAGGACCCACTGGAT-3′), WNK3-rev (5′-AGCCATTTCCAACATACACATC-3′), WNK4-for (5′-GCTGCAAACTCACAACAGCA-3′), and WNK4-rev (5′-CTCAGGAATCCGTCTCGCTC-3′).

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: .. Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific). .. Real-time PCR was performed on a Step One Plus sequence detector (Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: .. First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: .. The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol. .. For validation, quantitative real-time polymerase chain reaction was performed on a CFX-384 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA) using SYBR® Green Gene Expression Assays, and primer pairs were synthesized by Sigma-Aldrich (St. Louis, MO) or IDT (Coralville, IA).

    Quantitative RT-PCR:

    Article Title: Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean
    Article Snippet: Paragraph title: RNA extraction and quantitative RT-PCR ... Reverse transcription (RT) and first strand cDNA synthesis were carried out using Superscript II (Invitrogen, Carlsbad, CA).

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: Paragraph title: RT-qPCR. ... Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific).

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: HAV and NoV Genotyping The samples that tested positive for either HAV or NoV in real-time RT-PCR were subjected to further investigations for the identification of viral genotype. .. First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ).

    Real-time Polymerase Chain Reaction:

    Article Title: MacroH2A histone variants act as a barrier upon reprogramming towards pluripotency
    Article Snippet: .. A total of 1ug of RNA was used to synthesize cDNA using Superscript II and Oligo d(T) primers (Invitrogen). qPCR and mRNA analysis was carried out as described . cDNA expression was normalized to L7 levels. ..

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific). .. Real-time PCR was performed on a Step One Plus sequence detector (Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: Paragraph title: Real-time Quantitative PCR ... First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol. .. For validation, quantitative real-time polymerase chain reaction was performed on a CFX-384 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA) using SYBR® Green Gene Expression Assays, and primer pairs were synthesized by Sigma-Aldrich (St. Louis, MO) or IDT (Coralville, IA).

    Article Title: A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
    Article Snippet: Paragraph title: Real-time quantitative PCR analysis ... Briefly, 2.5 μg of total RNA was reverse transcribed using Superscript II (200 or 400 U, Invitrogen, Cergy Pontoise, France) with anchored-oligodT primer (8 mmol/L), random Hexamer (16 mmol/L), and deoxynucleotide triphosphates (500 μmol/L each).

    Nested PCR:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Incubation:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: .. PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. The RT reaction was carried out at 42°C for 50 min, followed by inactivation at 70°C for 15 min, after which RNase H was added and the mixture was incubated at 37°C for 30 min.

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: In vivo splicing assays: DNAsing, reverse transcription, PCR, and quantifying percent mRNA Isolated RNA (500 ng) was incubated with 1 unit of RQI DNase (Promega, Madison, WI, USA) in a 10 μl reaction for 2 h (LCAT experiments) or 1 h (GNPTG experiments) according to the manufacturer's protocol. .. DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA).

    Expressing:

    Article Title: MacroH2A histone variants act as a barrier upon reprogramming towards pluripotency
    Article Snippet: .. A total of 1ug of RNA was used to synthesize cDNA using Superscript II and Oligo d(T) primers (Invitrogen). qPCR and mRNA analysis was carried out as described . cDNA expression was normalized to L7 levels. ..

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: Paragraph title: Gene expression by real-time reverse transcription polymerase chain reaction ... The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Paragraph title: Gene expression profiling of Swedish-Norwegian samples ... Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    Modification:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: .. PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Countercurrent Chromatography:

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ). .. Primers for the hemi-nested PCR were 5′-CTG CCC GAA TTY GTA AAT GA-3 (G1-SKF; sense), 5′-CCA ACC CAR CCA TTR TAC A-3′ (G1-SKR; antisense), 5′-CNT GGG AGG GCG ATC GCA A-3′ (G2-SKF; sense), and 5′–CCR CCN GCA TRH CCR TTR TAC AT-3′ (G2-SKR; antisense).

    Sequencing:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: Sequencing libraries for each sample were prepared using the TruSeq RNA Sample Prep Kit-v2 from Illumina (Cambridge UK). .. The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK).

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: .. PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: .. DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA). .. For the LCAT splicing experiments, 2 μl of the reverse transcription reaction was subjected to 20 rounds of PCR amplification in a 20 μl reaction (1:10 dilution) using LCAT specific primers spiked with a kinased LCAT forward primer (0.4 nM).

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific). .. Real-time PCR was performed on a Step One Plus sequence detector (Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Indexed sequencing libraries were prepared using the TruSeq RNA sample preparation v2 kit in 96-well format (Illumina, San Diego, CA, USA). .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    Ligation:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK). .. A double stranded DNA generated from the cDNA was blunt ended before A-tailing and the ligation of barcoded adapters.

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA). .. End-repair, 3′-adenylation, ligation of indexed adaptors and PCR amplification was performed according to Illumina protocols.

    SYBR Green Assay:

    Article Title: Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean
    Article Snippet: Reverse transcription (RT) and first strand cDNA synthesis were carried out using Superscript II (Invitrogen, Carlsbad, CA). .. Actin was employed as an internal control to normalize the cDNA. qRT-PCR was carried out in 96-well plate using SYBR Green Mix .

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol. .. For validation, quantitative real-time polymerase chain reaction was performed on a CFX-384 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA) using SYBR® Green Gene Expression Assays, and primer pairs were synthesized by Sigma-Aldrich (St. Louis, MO) or IDT (Coralville, IA).

    Generated:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK). .. A double stranded DNA generated from the cDNA was blunt ended before A-tailing and the ligation of barcoded adapters.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: .. RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. The RT reaction was carried out at 42°C for 50 min, followed by inactivation at 70°C for 15 min, after which RNase H was added and the mixture was incubated at 37°C for 30 min.

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: Paragraph title: Gene expression by real-time reverse transcription polymerase chain reaction ... The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: .. First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ). .. For NoV, a hemi-nested RT-PCR protocol was used, targeting a highly conserved region in the diagnostic region C (ORF2) located on the capsid protein VP1, with minor modifications (Kojima et al. ; Nishida et al. ).

    Cellular Antioxidant Activity Assay:

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. Primers for TNFR2 were 5′-TAC CAA GGG TGG CAT CTC TC-3′ (forward) and 5′-TCC TGG GAT TTC TCA TCA GG-3′ (reverse), with the predicted size of the RT-PCR product being 171 bp.

    In Vivo:

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: Paragraph title: In vivo splicing assays: DNAsing, reverse transcription, PCR, and quantifying percent mRNA ... DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA).

    RNA Sequencing Assay:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: Paragraph title: RNA-sequencing ... The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK).

    Magnetic Beads:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. PCR amplicons were purified using magnetic beads (Agencourt AMPure XP, 1X concentration).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Between 0.1 and 1 µg of total RNA was used for poly-A mRNA capture using oligo-dT attached magnetic beads. .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    Isolation:

    Article Title: Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
    Article Snippet: Total RNA was isolated using RNAeasy columns (Qiagen) according to the manufacturer's instructions. .. A 1–0.5 µg total RNA was converted into cDNA using an anchored poly-dT primer and the Superscript II (human samples) or III (chicken samples) Reverse transcription kit (Invitrogen).

    Article Title: MacroH2A histone variants act as a barrier upon reprogramming towards pluripotency
    Article Snippet: Paragraph title: cDNA isolation and quantitative PCR ... A total of 1ug of RNA was used to synthesize cDNA using Superscript II and Oligo d(T) primers (Invitrogen). qPCR and mRNA analysis was carried out as described . cDNA expression was normalized to L7 levels.

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: In vivo splicing assays: DNAsing, reverse transcription, PCR, and quantifying percent mRNA Isolated RNA (500 ng) was incubated with 1 unit of RQI DNase (Promega, Madison, WI, USA) in a 10 μl reaction for 2 h (LCAT experiments) or 1 h (GNPTG experiments) according to the manufacturer's protocol. .. DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA).

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: .. First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: Total RNA was isolated from MBH, pituitary gland, testis, or epididymis using RNA extraction kit, Qiazol Reagent (miRNeasy; Qiagen, Hilden, Germany), and DNase treatment (RNase-free; Qiagen). .. The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Icelandic blood samples were collected in PAXgene tubes (PreAnalytix, Switzerland; cat no. #762165) and RNA was isolated using the PAXgene 96 Blood RNA or the Paxgene Blood RNA Kit (PreAnalytix; cat nos. .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    RNA Extraction:

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... First-strand cDNA was synthesized by reverse transcription of 1 μg of total RNA using Superscript-II and random primers (Invitrogen) according to standard protocols.

    Article Title: Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean
    Article Snippet: Paragraph title: RNA extraction and quantitative RT-PCR ... Reverse transcription (RT) and first strand cDNA synthesis were carried out using Superscript II (Invitrogen, Carlsbad, CA).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: Total RNA was isolated from MBH, pituitary gland, testis, or epididymis using RNA extraction kit, Qiazol Reagent (miRNeasy; Qiagen, Hilden, Germany), and DNase treatment (RNase-free; Qiagen). .. The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.

    Article Title: A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
    Article Snippet: Real-time quantitative PCR analysis RNA extraction, cDNA synthesis and qPCR assays were carried out as described previously (Befort et al. ). .. Briefly, 2.5 μg of total RNA was reverse transcribed using Superscript II (200 or 400 U, Invitrogen, Cergy Pontoise, France) with anchored-oligodT primer (8 mmol/L), random Hexamer (16 mmol/L), and deoxynucleotide triphosphates (500 μmol/L each).

    Purification:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. PCR amplicons were purified using magnetic beads (Agencourt AMPure XP, 1X concentration).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Gene expression profiling of Swedish-Norwegian samples For the Swedish-Norwegian samples, total RNA was purified from immune-magnetically isolated CD138+ cells using standard methods (Macherey Nagel NucleoSpin® RNA #740955.10 or QIAamp RNA blood #52304). .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    Polymerase Chain Reaction:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK). .. After removing un-ligated adapters the library was subjected to 11 cycles of PCR to increase the yield of products.

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. A total of 1 μL of from the cDNA synthesis reaction was used as template for nested PCR with Kapa HiFi PCR Kit.

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. Subsequently, PCR was performed, with initial denaturation at 94°C for 1 min, followed by 40 cycles of denaturation at 94°C for 15 s, annealing at 60°C for 1 min, and extension at 72°C for 30 s, with a final extension at 72°C for 8 min. Primers for mouse TNFR1 were 5′-CAG TCT GCA GGG AGT GTG AA-3′ (forward) and 5′-CAC GCA CTG GAA GTG TGT CT-3′ (reverse) [ ], with a predicted size of 197 bp.

    Article Title: Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean
    Article Snippet: Reverse transcription (RT) and first strand cDNA synthesis were carried out using Superscript II (Invitrogen, Carlsbad, CA). .. Primers were designed to amplify gene specific PCR products of < 200 bp in size.

    Article Title: Identification of motifs that function in the splicing of non-canonical introns
    Article Snippet: Paragraph title: In vivo splicing assays: DNAsing, reverse transcription, PCR, and quantifying percent mRNA ... DNAsed RNA (2 μl (100 ng)) was reverse transcribed in a 10 μl reaction (1:5 dilution) using Superscript II and an LCAT-specific reverse primer or a reverse primer to the pCDNA3 SP6 sequence for the GNPTG experiments, according to manufacturer's protocols with the exception that we used half the recommended amount of Superscript II (Invitrogen, Carlsbad, CA, USA).

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific). .. Real-time PCR was performed on a Step One Plus sequence detector (Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ). .. Primers for the first PCR were as follows: 5′-CGY TGG ATG CGN TTY CAT GA-3′ (COG1F; sense), 5′-CCA ACC CAR CCA TTR TAC A-3′ (G1-SKR; antisense), 5′-CAR GAR BCN ATG TTY AGR TGG ATG AG-3′ (COG2F; sense), and 5′-CCR CCN GCA TRH CCR TTR TAC AT-3 (G2-SKR; antisense).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA). .. End-repair, 3′-adenylation, ligation of indexed adaptors and PCR amplification was performed according to Illumina protocols.

    IA:

    Article Title: The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor
    Article Snippet: First-strand complementary DNA was synthesized from isolated RNA using SuperScript II reverse transcription (18064014; Invitrogen). .. Prepared cDNA was mixed with 2 × SYBR Green PCR Master Mix (4309155; Applied Biosystems) and various sets of gene-specific forward and reverse primers (LXRα, LXRβ, ABCA1, ABCG1, IL1β, IL-6, SIRT1 and SREBP-1c (Integrated DNA Technologies; Coralville, IA) and subjected to real-time PCR quantification using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol. .. For validation, quantitative real-time polymerase chain reaction was performed on a CFX-384 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA) using SYBR® Green Gene Expression Assays, and primer pairs were synthesized by Sigma-Aldrich (St. Louis, MO) or IDT (Coralville, IA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. Primers for TNFR2 were 5′-TAC CAA GGG TGG CAT CTC TC-3′ (forward) and 5′-TCC TGG GAT TTC TCA TCA GG-3′ (reverse), with the predicted size of the RT-PCR product being 171 bp.

    Article Title: Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy)
    Article Snippet: First-round RT-PCR was performed using superscript II one step (Invitrogen, Paisley, UK) in order to amplify a 267-bp fragment (Robertson et al. ). .. Primers for the first PCR were as follows: 5′-CGY TGG ATG CGN TTY CAT GA-3′ (COG1F; sense), 5′-CCA ACC CAR CCA TTR TAC A-3′ (G1-SKR; antisense), 5′-CAR GAR BCN ATG TTY AGR TGG ATG AG-3′ (COG2F; sense), and 5′-CCR CCN GCA TRH CCR TTR TAC AT-3 (G2-SKR; antisense).

    Chromatin Immunoprecipitation:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK). .. The final sequencing libraries were assessed using electrophoresis on an Agilent Bioanalyser DNA 1000 chip (Agilent, Stockport, UK) to identify the size of the library products; the average library size was 328 ± 8 bases.

    Electrophoresis:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK). .. The final sequencing libraries were assessed using electrophoresis on an Agilent Bioanalyser DNA 1000 chip (Agilent, Stockport, UK) to identify the size of the library products; the average library size was 328 ± 8 bases.

    Negative Control:

    Article Title: Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity
    Article Snippet: The complementary DNA was synthesized using Superscript II and random primers (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol. .. Water instead of complementary DNA was used as a negative control, and Gapdh was used as housekeeping gene.

    Sample Prep:

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: Sequencing libraries for each sample were prepared using the TruSeq RNA Sample Prep Kit-v2 from Illumina (Cambridge UK). .. The fragmented RNA was reverse transcribed using a random primer cocktail provided in the kit and SuperScript II (Life Technologies, Paisley UK).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: Indexed sequencing libraries were prepared using the TruSeq RNA sample preparation v2 kit in 96-well format (Illumina, San Diego, CA, USA). .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA).

    Random Hexamer Labeling:

    Article Title: Nipah virus persists in the brains of nonhuman primate survivors
    Article Snippet: .. Briefly, cDNA was synthesized by using total RNA extracted from PBMCs with Superscript II and random hexamer priming (Thermo Fisher Scientific). .. Real-time PCR was performed on a Step One Plus sequence detector (Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

    Article Title: The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression
    Article Snippet: .. Complementary DNA synthesis was done using SuperScript II and random hexamer priming (ThermoFisher, Waltham, MA, USA). .. End-repair, 3′-adenylation, ligation of indexed adaptors and PCR amplification was performed according to Illumina protocols.

    Article Title: A mu–delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks
    Article Snippet: .. Briefly, 2.5 μg of total RNA was reverse transcribed using Superscript II (200 or 400 U, Invitrogen, Cergy Pontoise, France) with anchored-oligodT primer (8 mmol/L), random Hexamer (16 mmol/L), and deoxynucleotide triphosphates (500 μmol/L each). .. Real-time PCR was performed in triplicate on a MyIQ BioRad instrument using iQSYBRGreen supermix (Bio-Rad, Marnes-la-Coquette, France), cDNA (0.5 μL), and gene-specific primers (200 nmol/L) in a 25 μL reaction as recommended by the manufacturer.

    Concentration Assay:

    Article Title: The birth of a human-specific neural gene by incomplete duplication and gene fusion
    Article Snippet: PacBio cDNA sequencing cDNA was synthesized from poly-A+ RNA using oligo(dT) priming either SuperScript II or SuperScript III reverse transcriptase (Invitrogen) with the following modification: an extra 50 min was added to the reverse transcription incubation time following the addition of another 1 μL of enzyme. .. PCR amplicons were purified using magnetic beads (Agencourt AMPure XP, 1X concentration).

    CTG Assay:

    Article Title: Effects of Tumor Necrosis Factor-α on Podocyte Expression of Monocyte Chemoattractant Protein-1 and in Diabetic Nephropathy
    Article Snippet: RT-PCR Podocyte RNA was treated with DNase I to clear contaminating genomic DNA and reverse-transcribed with 100 U Superscript II (Invitrogen, Carlsbad, Calif., USA), 0.5 μg gene-specific primer, 20 U RNase inhibitor, and 1 mM dNTPs. .. Subsequently, PCR was performed, with initial denaturation at 94°C for 1 min, followed by 40 cycles of denaturation at 94°C for 15 s, annealing at 60°C for 1 min, and extension at 72°C for 30 s, with a final extension at 72°C for 8 min. Primers for mouse TNFR1 were 5′-CAG TCT GCA GGG AGT GTG AA-3′ (forward) and 5′-CAC GCA CTG GAA GTG TGT CT-3′ (reverse) [ ], with a predicted size of 197 bp.

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  • 90
    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase/product/Thermo Fisher
    Average 90 stars, based on 805 article reviews
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    superscript ii reverse transcriptase - by Bioz Stars, 2020-01
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