superscript ii cdna synthesis kit thermo fisher scientific  (Thermo Fisher)


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    Name:
    SuperScript Double Stranded cDNA Synthesis Kit
    Description:
    The SuperScript Double Stranded cDNA Synthesis Kit contains all of the reagents except an oligo dT containing primer necessary to make high quality double stranded cDNA from total RNA or poly A selected RNA mRNA The advanced capabilities of SuperScript II Reverse Transcriptase with its reduced RNase H activity maximize the yield of full length cDNA as well as that of overall cDNA synthesis Features of the SuperScript Double Stranded cDNA Synthesis Kit include • Reliability each buffer reagent and enzyme in the kit is of the same high quality you expect from us• Performance SuperScript II is an enzyme you can trust for high yield high quality general purpose first strand synthesis Simple reliable and trouble freeThe SuperScript Double Stranded cDNA Synthesis Kit offers a simple reliable and trouble free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments The reactions described in the SuperScript Double Stranded cDNA Synthesis Kit protocol are designed to convert 25 50 µg total RNA or 0 2 5 µg mRNA into first and second strand cDNA supply your own primer Enjoy the benefits of a tested protocol and reagents of consistent quality To get the best possible cDNA product using this kit we recommend the use of a high quality RNA substrate prepared using TRIzol RNA Isolation Reagents
    Catalog Number:
    11917010
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Labeling|Nucleic Acid Labeling & Oligo Synthesis|PCR & Real-Time PCR|Reverse Transcription|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher superscript ii cdna synthesis kit thermo fisher scientific
    The SuperScript Double Stranded cDNA Synthesis Kit contains all of the reagents except an oligo dT containing primer necessary to make high quality double stranded cDNA from total RNA or poly A selected RNA mRNA The advanced capabilities of SuperScript II Reverse Transcriptase with its reduced RNase H activity maximize the yield of full length cDNA as well as that of overall cDNA synthesis Features of the SuperScript Double Stranded cDNA Synthesis Kit include • Reliability each buffer reagent and enzyme in the kit is of the same high quality you expect from us• Performance SuperScript II is an enzyme you can trust for high yield high quality general purpose first strand synthesis Simple reliable and trouble freeThe SuperScript Double Stranded cDNA Synthesis Kit offers a simple reliable and trouble free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments The reactions described in the SuperScript Double Stranded cDNA Synthesis Kit protocol are designed to convert 25 50 µg total RNA or 0 2 5 µg mRNA into first and second strand cDNA supply your own primer Enjoy the benefits of a tested protocol and reagents of consistent quality To get the best possible cDNA product using this kit we recommend the use of a high quality RNA substrate prepared using TRIzol RNA Isolation Reagents
    https://www.bioz.com/result/superscript ii cdna synthesis kit thermo fisher scientific/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript ii cdna synthesis kit thermo fisher scientific - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Filtration:

    Article Title: Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms
    Article Snippet: .. After removal of hydrolysis ions by G50 Sephadex filtration (USA Scientific catalog # 1415-1602), the fragmented mRNA was random primed with hexamers and reverse-transcribed using the Super Script II cDNA synthesis kit (Invitrogen catalog # 11917010). .. After second strand synthesis, the cDNA went through end-repair and ligation reactions according to the Illumina ChIP-Seq genomic DNA preparation kit protocol (Illumina catalog # IP102-1001), using the paired end adapters and amplification primers (Illumina Catalog # PE102-1004).

    Synthesized:

    Article Title: MasABK Proteins Interact with Proteins of the Type IV Pilin System to Affect Social Motility of Myxococcus xanthus
    Article Snippet: .. Double-stranded cDNAs were synthesized using Invitrogen's SuperScript cDNA Synthesis Kit with the standard protocol using random hexanucleotide primers (Invitrogen, Carlsbad, CA). .. RNA was harvested from WT, MxH2604 and MxH2627 grown in liquid culture to a density of 5×108 cells/ml as described elsewhere . cDNAs were fluorescently labeled with Cy3 using the standard NimbleGen Gene Expression Analysis protocol.

    Article Title: Differential Gene Expression in CD8+ Cells from HIV-1 Infected Subjects Showing Suppression of HIV Replication
    Article Snippet: .. Complementary DNA (cDNA) was synthesized with the SuperScript™ Double Stranded cDNA Synthesis Kit (Invitrogen Corporation, Carlsbad, CA, USA) using an oligo (dT) primer containing the T7 RNA polymerase site. .. The cDNA was then subjected to an in vitro transcription (IVT) reaction using the T7 RNA polymerase to generate a biotin-labeled complementary RNA (cRNA) (Enzo BioArray™ High Yield™ RNA Transcript Labeling kit, Enzo Life Sciences Inc, Farmingdale, NY, USA) according to the manufacturer instructions.

    Article Title: The E2A-HLF Oncoprotein Activates Groucho-Related Genes and Suppresses Runx1
    Article Snippet: .. Double-stranded cDNAs were synthesized with the Superscript cDNA synthesis kit (GIBCO-BRL) according to the manufacturer's protocol. .. Double-stranded cDNA (2 μg) isolated from both cells grown in the presence of zinc and cells deprived of zinc was digested with Dpn II, ligated with adapter, and amplified by PCR ( , ).

    Random Primed:

    Article Title: Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms
    Article Snippet: .. After removal of hydrolysis ions by G50 Sephadex filtration (USA Scientific catalog # 1415-1602), the fragmented mRNA was random primed with hexamers and reverse-transcribed using the Super Script II cDNA synthesis kit (Invitrogen catalog # 11917010). .. After second strand synthesis, the cDNA went through end-repair and ligation reactions according to the Illumina ChIP-Seq genomic DNA preparation kit protocol (Illumina catalog # IP102-1001), using the paired end adapters and amplification primers (Illumina Catalog # PE102-1004).

    Spectrophotometry:

    Article Title: Conserved Subgroups and Developmental Regulation in the Monocot rop Gene Family 1 Gene Family 1 [w]
    Article Snippet: .. RNA concentrations were determined by spectrophotometry, and cDNA was generated from 5 μg of total RNA using oligo-d(T) primers and the SuperScript cDNA Synthesis kit (Invitrogen). .. To determine relative expression levels of the rop genes, we used MTRP ( ) with each of the three primer sets.

    Generated:

    Article Title: Conserved Subgroups and Developmental Regulation in the Monocot rop Gene Family 1 Gene Family 1 [w]
    Article Snippet: .. RNA concentrations were determined by spectrophotometry, and cDNA was generated from 5 μg of total RNA using oligo-d(T) primers and the SuperScript cDNA Synthesis kit (Invitrogen). .. To determine relative expression levels of the rop genes, we used MTRP ( ) with each of the three primer sets.

    cDNA-AFLP Assay:

    Article Title: Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of ‘Président Roulin’ against Venturia inaequalis
    Article Snippet: .. Double stranded cDNA was finally obtained starting from 500 ng mRNA following the instructions of the Superscript Double Stranded cDNA Synthesis kit (Invitrogen Inc.). cDNA-AFLP analysis was performed with the AFLP Core Reagent kit (Invitrogen Inc.) as recommended by the manufacturer. ..

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  • 99
    Thermo Fisher cdna synthesis kit superscript ii
    Cdna Synthesis Kit Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis kit superscript ii/product/Thermo Fisher
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    Thermo Fisher superscript ii 2 synthesis kit
    Superscript Ii 2 Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii 2 synthesis kit/product/Thermo Fisher
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    Price from $9.99 to $1999.99
    superscript ii 2 synthesis kit - by Bioz Stars, 2020-08
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