supersciptiii reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher supersciptiii reverse transcriptase
    Supersciptiii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/supersciptiii reverse transcriptase/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    supersciptiii reverse transcriptase - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

    trizol
    rna
    allprep kit
    cdna
    cdna synthesis

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    Related Articles

    Molecular Cloning:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: Paragraph title: RNA extraction and molecular cloning of viral genomic cDNA fragments ... The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ).

    RNA Extraction:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: Paragraph title: RNA extraction and molecular cloning of viral genomic cDNA fragments ... The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ).

    Amplification:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. Six fragments (designated as A, B, C, D, E and F. Figure ). spanning the full-length genome, were subsequently generated by PCR amplification with Platinum® Taq DNA polymerase.

    Clone Assay:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. Each PCR product was cloned into pCR-Blunt II-TOPO vector (Invitrogen).

    Isolation:

    Article Title: Upregulation of BDNF NGF in Cervical Intervertebral Discs Exposed to Painful Whole Body Vibration
    Article Snippet: .. RNA was isolated with Trizol (Life Technologies; Carlsbad, CA) or the Allprep kit (Qiagen; Germantown, MD) and cDNA was prepared using SupersciptIII Reverse Transcriptase (Life Technologies). ..

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: RNA extraction and molecular cloning of viral genomic cDNA fragments Total RNAs were isolated from the supernatants of MARC-145 cells culture at 48 h post infection (PI) by using a QIAamp viral RNA kit (QIAGEN). .. The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ).

    Quantitative RT-PCR:

    Article Title: Upregulation of BDNF NGF in Cervical Intervertebral Discs Exposed to Painful Whole Body Vibration
    Article Snippet: At day 14, cervical intervertebral discs (C5-C8) were harvested to quantify BDNF and NGF mRNA levels using RT-qPCR. .. RNA was isolated with Trizol (Life Technologies; Carlsbad, CA) or the Allprep kit (Qiagen; Germantown, MD) and cDNA was prepared using SupersciptIII Reverse Transcriptase (Life Technologies).

    SYBR Green Assay:

    Article Title: Upregulation of BDNF NGF in Cervical Intervertebral Discs Exposed to Painful Whole Body Vibration
    Article Snippet: RNA was isolated with Trizol (Life Technologies; Carlsbad, CA) or the Allprep kit (Qiagen; Germantown, MD) and cDNA was prepared using SupersciptIII Reverse Transcriptase (Life Technologies). .. Power Sybr Green (Applied Biosystems; Carlsbad, CA) was used for RT-qPCR on the 7300 System (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. The PCR primers in Table were designed mainly according to the sequence of PRRSV HuN4 strain with the GeneBank accession number of EF635006 [ ].

    Generated:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. Six fragments (designated as A, B, C, D, E and F. Figure ). spanning the full-length genome, were subsequently generated by PCR amplification with Platinum® Taq DNA polymerase.

    Infection:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: RNA extraction and molecular cloning of viral genomic cDNA fragments Total RNAs were isolated from the supernatants of MARC-145 cells culture at 48 h post infection (PI) by using a QIAamp viral RNA kit (QIAGEN). .. The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ).

    Sequencing:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. The PCR primers in Table were designed mainly according to the sequence of PRRSV HuN4 strain with the GeneBank accession number of EF635006 [ ].

    Plasmid Preparation:

    Article Title: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
    Article Snippet: The cDNA synthesis was performed with SupersciptIII reverse transcriptase (Invitrogen) and reverse primers of each fragment (Table ). .. Each PCR product was cloned into pCR-Blunt II-TOPO vector (Invitrogen).

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  • 99
    Thermo Fisher superscriptiii reverse transcriptase
    Superscriptiii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscriptiii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 3316 article reviews
    Price from $9.99 to $1999.99
    superscriptiii reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars
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