Structured Review

Stratagene supercompetent e coli xl1 blue cells
Supercompetent E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supercompetent e coli xl1 blue cells/product/Stratagene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
supercompetent e coli xl1 blue cells - by Bioz Stars, 2020-04
86/100 stars

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Electroporation:

Article Title: Plasmid Transduction Using Bacteriophage ?adh for Expression of CC Chemokines by Lactobacillus gasseri ADH ▿
Article Snippet: The plasmid pBR322 was purchased from Fermentas, Canada, while transformations were carried out using supercompetent E. coli XL1-Blue cells per the instructions of the supplier (Stratagene). .. All L. gasseri ADH electroporation-derived transformants were selected on and propagated in MRS plates and MRS broth containing 9 μg/ml chloramphenicol at 37°C.

Clone Assay:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). .. Numerous sigma clones migrated to lower positions on protein gels, especially from W434A screens, and these were not studied further.

Mutagenesis:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: .. rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). ..

Construct:

Article Title: Plasmid Transduction Using Bacteriophage ?adh for Expression of CC Chemokines by Lactobacillus gasseri ADH ▿
Article Snippet: The plasmid pBR322 was purchased from Fermentas, Canada, while transformations were carried out using supercompetent E. coli XL1-Blue cells per the instructions of the supplier (Stratagene). .. Transformants containing pBR322-based constructs were selected on and propagated in LB plates and LB broth, respectively, supplemented with 0.1 mg/ml ampicillin, while transformants containing pGK12-based constructs were selected on and propagated in LB plates and LB broth containing 2 μg/ml chloramphenicol and 50 μg/ml erythromycin or 7 μg/ml chloramphenicol alone at 37°C.

Migration:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). .. Colonies were screened for isopropyl-β- d -thiogalactopyranoside (IPTG)-induced sigma from sonicated extracts with proper migration by SDS–PAGE.

Sonication:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). .. Colonies were screened for isopropyl-β- d -thiogalactopyranoside (IPTG)-induced sigma from sonicated extracts with proper migration by SDS–PAGE.

Transformation Assay:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: .. rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). ..

SDS Page:

Article Title: Escherichia coli promoter opening and -10 recognition: mutational analysis of ?70
Article Snippet: rpoD mutants were made using pQE30-rpoD ( ) by site-directed mutagenesis and transformed into supercompetent E.coli XL1 Blue cells (Stratagene). .. Colonies were screened for isopropyl-β- d -thiogalactopyranoside (IPTG)-induced sigma from sonicated extracts with proper migration by SDS–PAGE.

Plasmid Preparation:

Article Title: Plasmid Transduction Using Bacteriophage ?adh for Expression of CC Chemokines by Lactobacillus gasseri ADH ▿
Article Snippet: .. The plasmid pBR322 was purchased from Fermentas, Canada, while transformations were carried out using supercompetent E. coli XL1-Blue cells per the instructions of the supplier (Stratagene). .. Transformants containing pBR322-based constructs were selected on and propagated in LB plates and LB broth, respectively, supplemented with 0.1 mg/ml ampicillin, while transformants containing pGK12-based constructs were selected on and propagated in LB plates and LB broth containing 2 μg/ml chloramphenicol and 50 μg/ml erythromycin or 7 μg/ml chloramphenicol alone at 37°C.

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    Stratagene xl1 blue supercompetent e coli cells
    Xl1 Blue Supercompetent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xl1 blue supercompetent e coli cells/product/Stratagene
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xl1 blue supercompetent e coli cells - by Bioz Stars, 2020-04
    93/100 stars
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