superase in rnase inhibitor  (New England Biolabs)


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    Structured Review

    New England Biolabs superase in rnase inhibitor
    Superase In Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in rnase inhibitor/product/New England Biolabs
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superase in rnase inhibitor - by Bioz Stars, 2019-12
    91/100 stars

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    Isolation:

    Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
    Article Snippet: The recovered ribosome-bound RNA was isolated by using TRIzol and the remaining rRNAs were removed with the Ribo-Zero™ rRNA Removal Kit for Meta-bacteria. .. For the phosphorylation reaction, samples were denatured at 80 °C for 90 s, equilibrated to 37 °C, and incubated at 37 °C for 1 h with 5 μL of 10× T4 PNK buffer (NEB), 20 U SUPERase-In RNase Inhibitor, and 10 U T4 PNK (NEB).

    Incubation:

    Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
    Article Snippet: The recovered ribosome-bound RNA was isolated by using TRIzol and the remaining rRNAs were removed with the Ribo-Zero™ rRNA Removal Kit for Meta-bacteria. .. For the phosphorylation reaction, samples were denatured at 80 °C for 90 s, equilibrated to 37 °C, and incubated at 37 °C for 1 h with 5 μL of 10× T4 PNK buffer (NEB), 20 U SUPERase-In RNase Inhibitor, and 10 U T4 PNK (NEB). .. After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen).

    Construct:

    Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
    Article Snippet: For the phosphorylation reaction, samples were denatured at 80 °C for 90 s, equilibrated to 37 °C, and incubated at 37 °C for 1 h with 5 μL of 10× T4 PNK buffer (NEB), 20 U SUPERase-In RNase Inhibitor, and 10 U T4 PNK (NEB). .. After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen).

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  • 91
    New England Biolabs superase in rnase inhibitor
    Superase In Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in rnase inhibitor/product/New England Biolabs
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    superase in rnase inhibitor - by Bioz Stars, 2019-12
    91/100 stars
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