superase in  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs superase in
    Superase In, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in/product/New England Biolabs
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    superase in - by Bioz Stars, 2020-07
    93/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
    Article Snippet: .. To anneal the RTprimer, the mixture was incubated at 75 °C for 5 min, 37 °C for 15 min and 25 °C for 10 min. To ligate the 5′ Illumina TruSeq adapter, 10 μL 5′master mix (1.5 μL dH2 O + 0.2% Tween 20, 0.25 μL denaturated 5′TruSeq adapter (10 μM), 1.5 μL 10× T4 RNA ligase buffer, 0.25 μL SUPERase-In, 0.2 μL 10 mM ATP, 5.8 μL 50% PEG8000, 0.5 μL T4 RNA ligase 1 (5U; NEB)) was added and reactions were incubated at 25 °C for 1 h. Reverse transcription was performed using Protoscript II (NEB) (4 μL 5× NEB FirstStrand buffer (NEB; E7421AA), 0.25 μL SUPERase-In, 0.75 μL Protoscript II (150U; NEB)) at 50 °C for 1 h. After addition of 30 μL PCR master mix (25 μL 2× LongAmp Taq 2× Master Mix (NEB), 0.2 μL 100 μM forward primer, 2.8 μL 5 M betaine and 2 μL 10 μM individual barcoding primer), mixtures were amplified (95 °C for 3 min, (95 °C for 60 s, 62 °C for 30 s, 72 °C for 15 s) x13, 72 °C for 3 min). .. PCR reactions were cleaned up using 1.5 volumes of SpeedBeads (GE Healthcare) in 2.5 M NaCl/20% PEG8000.

    Purification:

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: .. Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). ..

    Electrophoresis:

    Article Title: Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation in vivo
    Article Snippet: .. Solutions and reagents: Mitoribosome-associated RNA (from Basic Protocol 1) and/or fragmented and size-selected mRNA (from Support Protocol) 2X urea RNA loading buffer (see recipe) 10-bp DNA ladder 15%, 10% TBE-urea, 8% TBE polyacrylamide gels SYBR gold (10,000X concentrate) RNase-free water 3 M sodium acetate, pH 5.5 (RNase-free) 25 mg/ml linear polyacrylamide (LPA) Isopropanol 70% (v/v) ethanol 10X T4 polynucleotide kinase (PNK) buffer 10 U/μl T4 PNK Oligonucleotide linker and primers (see Table) 50% (w/v) PEG 8000 (RNase-free) 10X T4 RNA ligase reaction buffer (NEB, B0216L) 200 U/μl T4 RNA ligase 2, truncated (NEB) 10 mM Tris, pH 8.0 (RNase-free) 5X First-strand (FS) RT reaction buffer (Invitrogen, 18080-993) 10 mM dNTPs 0.1 M DTT 20 U/μl SUPERase-In 200 U/μl Superscript III 1 N sodium hydroxide 3 M sodium chloride 10X CircLigase reaction buffer (Epicentre, CL4111K) 1 mM ATP 50 mM manganese chloride 100 U/μl CircLigase ssDNA ligase 5X Phusion HF reaction buffer (NEB, M0530S) 2 U/μl Phusion HF DNA polymerase 10X DNA loading buffer (see recipe) 100-bp DNA ladder DNA gel extraction buffer (see recipe) 10 mM Tris, pH 8.5 Qubit dsDNA HS Assay kit Special equipment: Heat blocks: 80°C, 70°C, 37°C, 25°C Typhoon fluorescent/phosphorescent image scanner, or UV transilluminator with camera Blue light transilluminator (or UV transilluminator) box End over end rotator Costar Spin-X centrifuge tube filter (0.45-um cellulose acetate in 2-ml tube; Corning) Qubit Fluorometer Bioanalyzer DNA high sensitivity chip and reagents Other equipment: Polyacrylamide electrophoresis apparatus and power source 0.5-ml and 1.5-ml RNase-free non-stick microcentrifuge tubes Plastic sheet protector Razor blades or scalpels Thermocycler Plastic sheet protector .. 1 Combine 10 μl RNA from large scale mitoribosome purification with 10 μl 2X urea RNA loading buffer.

    Concentration Assay:

    Article Title: In vitro Reconstitution Assay of miRNA Biogenesis by Arabidopsis DCL1
    Article Snippet: .. Mix the following in a fresh tube at 4 °C: 4.5 μl of RNA (from step B), 1 μl of 10x NEB T4 kinase buffer, 3 μl of [γ-32 P]-ATP (3,000 Ci/mmol 10 mCi/ml), 0.5 μl of Superase-In (final concentration 1 U/μl) and 1 μl of NEB T4 kinase. .. Incubate the reaction mixture at 37 °C for 2 h. Add 230 μl of DEPC H2 O to the above reaction mixture.

    Incubation:

    Article Title: A CRE/AP-1-Like Motif Is Essential for Induced Syncytin-2 Expression and Fusion in Human Trophoblast-Like Model
    Article Snippet: .. RNA (1 μg) was then incubated in the presence of oligo(dT) (25 ng/μl), 10 mM DTT, 100 μM dNTP (deoxynucleotide triphosphate), SuperScript reverse transcriptase (10 U) (Invitrogen Canada Inc), and SUPERase-In (20 U) at 42°C for 50 min. Aliquots from the RT reactions were then PCR-amplified in the presence of 2 U Vent DNA polymerase (New England Biolabs, Pickering, Canada), 1× ThermoPol buffer, 100 μM dNTP, and 15 μM of each primer. .. PCR amplification was performed using Syncytin-2-specific primers 5’-CCTTCACTAGCAGCCTACCG-3’ (forward) and 5’-GCTGTCCCTGGTGTTTCAGT-3’ (reverse); CREB2-specific primers 5’-GTGTCATCCAACGTGGTCAG-3’ (forward) and 5’-CCAACAACAGCAAGGAGGAT-3’ (reverse); and JunD-specific primers 5’-GTGCCCAGGAACTCAGAGAG-3’ (forward) and 5’-CCAACGTTTGTTCCCGAGTA-3’ (reverse).

    Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
    Article Snippet: .. To anneal the RTprimer, the mixture was incubated at 75 °C for 5 min, 37 °C for 15 min and 25 °C for 10 min. To ligate the 5′ Illumina TruSeq adapter, 10 μL 5′master mix (1.5 μL dH2 O + 0.2% Tween 20, 0.25 μL denaturated 5′TruSeq adapter (10 μM), 1.5 μL 10× T4 RNA ligase buffer, 0.25 μL SUPERase-In, 0.2 μL 10 mM ATP, 5.8 μL 50% PEG8000, 0.5 μL T4 RNA ligase 1 (5U; NEB)) was added and reactions were incubated at 25 °C for 1 h. Reverse transcription was performed using Protoscript II (NEB) (4 μL 5× NEB FirstStrand buffer (NEB; E7421AA), 0.25 μL SUPERase-In, 0.75 μL Protoscript II (150U; NEB)) at 50 °C for 1 h. After addition of 30 μL PCR master mix (25 μL 2× LongAmp Taq 2× Master Mix (NEB), 0.2 μL 100 μM forward primer, 2.8 μL 5 M betaine and 2 μL 10 μM individual barcoding primer), mixtures were amplified (95 °C for 3 min, (95 °C for 60 s, 62 °C for 30 s, 72 °C for 15 s) x13, 72 °C for 3 min). .. PCR reactions were cleaned up using 1.5 volumes of SpeedBeads (GE Healthcare) in 2.5 M NaCl/20% PEG8000.

    Article Title: Analysis of genetically diverse macrophages reveals local and domain-wide mechanisms that control transcription factor binding and function
    Article Snippet: .. Afterwards, to ligate the 3′ Illumina TruSeq adapter 10 μl of 3′MM was added [1 μl 10x T4 RNA ligase buffer, 6 μl 50% PEG8000, 1.5 μl ddH2 O+T, 0.25 μl heat-denaturated Illumina TruSeq 3′Adapter, 0.25 μl SUPERase-In, 1 μl T4 RNA Ligase 2 truncated K227Q (200U; NEB)], mixed well by flicking and reactions incubated at 20°C for 1 hour. ..

    Polymerase Chain Reaction:

    Article Title: A CRE/AP-1-Like Motif Is Essential for Induced Syncytin-2 Expression and Fusion in Human Trophoblast-Like Model
    Article Snippet: .. RNA (1 μg) was then incubated in the presence of oligo(dT) (25 ng/μl), 10 mM DTT, 100 μM dNTP (deoxynucleotide triphosphate), SuperScript reverse transcriptase (10 U) (Invitrogen Canada Inc), and SUPERase-In (20 U) at 42°C for 50 min. Aliquots from the RT reactions were then PCR-amplified in the presence of 2 U Vent DNA polymerase (New England Biolabs, Pickering, Canada), 1× ThermoPol buffer, 100 μM dNTP, and 15 μM of each primer. .. PCR amplification was performed using Syncytin-2-specific primers 5’-CCTTCACTAGCAGCCTACCG-3’ (forward) and 5’-GCTGTCCCTGGTGTTTCAGT-3’ (reverse); CREB2-specific primers 5’-GTGTCATCCAACGTGGTCAG-3’ (forward) and 5’-CCAACAACAGCAAGGAGGAT-3’ (reverse); and JunD-specific primers 5’-GTGCCCAGGAACTCAGAGAG-3’ (forward) and 5’-CCAACGTTTGTTCCCGAGTA-3’ (reverse).

    Article Title: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages
    Article Snippet: .. To anneal the RTprimer, the mixture was incubated at 75 °C for 5 min, 37 °C for 15 min and 25 °C for 10 min. To ligate the 5′ Illumina TruSeq adapter, 10 μL 5′master mix (1.5 μL dH2 O + 0.2% Tween 20, 0.25 μL denaturated 5′TruSeq adapter (10 μM), 1.5 μL 10× T4 RNA ligase buffer, 0.25 μL SUPERase-In, 0.2 μL 10 mM ATP, 5.8 μL 50% PEG8000, 0.5 μL T4 RNA ligase 1 (5U; NEB)) was added and reactions were incubated at 25 °C for 1 h. Reverse transcription was performed using Protoscript II (NEB) (4 μL 5× NEB FirstStrand buffer (NEB; E7421AA), 0.25 μL SUPERase-In, 0.75 μL Protoscript II (150U; NEB)) at 50 °C for 1 h. After addition of 30 μL PCR master mix (25 μL 2× LongAmp Taq 2× Master Mix (NEB), 0.2 μL 100 μM forward primer, 2.8 μL 5 M betaine and 2 μL 10 μM individual barcoding primer), mixtures were amplified (95 °C for 3 min, (95 °C for 60 s, 62 °C for 30 s, 72 °C for 15 s) x13, 72 °C for 3 min). .. PCR reactions were cleaned up using 1.5 volumes of SpeedBeads (GE Healthcare) in 2.5 M NaCl/20% PEG8000.

    Gel Extraction:

    Article Title: Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation in vivo
    Article Snippet: .. Solutions and reagents: Mitoribosome-associated RNA (from Basic Protocol 1) and/or fragmented and size-selected mRNA (from Support Protocol) 2X urea RNA loading buffer (see recipe) 10-bp DNA ladder 15%, 10% TBE-urea, 8% TBE polyacrylamide gels SYBR gold (10,000X concentrate) RNase-free water 3 M sodium acetate, pH 5.5 (RNase-free) 25 mg/ml linear polyacrylamide (LPA) Isopropanol 70% (v/v) ethanol 10X T4 polynucleotide kinase (PNK) buffer 10 U/μl T4 PNK Oligonucleotide linker and primers (see Table) 50% (w/v) PEG 8000 (RNase-free) 10X T4 RNA ligase reaction buffer (NEB, B0216L) 200 U/μl T4 RNA ligase 2, truncated (NEB) 10 mM Tris, pH 8.0 (RNase-free) 5X First-strand (FS) RT reaction buffer (Invitrogen, 18080-993) 10 mM dNTPs 0.1 M DTT 20 U/μl SUPERase-In 200 U/μl Superscript III 1 N sodium hydroxide 3 M sodium chloride 10X CircLigase reaction buffer (Epicentre, CL4111K) 1 mM ATP 50 mM manganese chloride 100 U/μl CircLigase ssDNA ligase 5X Phusion HF reaction buffer (NEB, M0530S) 2 U/μl Phusion HF DNA polymerase 10X DNA loading buffer (see recipe) 100-bp DNA ladder DNA gel extraction buffer (see recipe) 10 mM Tris, pH 8.5 Qubit dsDNA HS Assay kit Special equipment: Heat blocks: 80°C, 70°C, 37°C, 25°C Typhoon fluorescent/phosphorescent image scanner, or UV transilluminator with camera Blue light transilluminator (or UV transilluminator) box End over end rotator Costar Spin-X centrifuge tube filter (0.45-um cellulose acetate in 2-ml tube; Corning) Qubit Fluorometer Bioanalyzer DNA high sensitivity chip and reagents Other equipment: Polyacrylamide electrophoresis apparatus and power source 0.5-ml and 1.5-ml RNase-free non-stick microcentrifuge tubes Plastic sheet protector Razor blades or scalpels Thermocycler Plastic sheet protector .. 1 Combine 10 μl RNA from large scale mitoribosome purification with 10 μl 2X urea RNA loading buffer.

    Chromatin Immunoprecipitation:

    Article Title: Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation in vivo
    Article Snippet: .. Solutions and reagents: Mitoribosome-associated RNA (from Basic Protocol 1) and/or fragmented and size-selected mRNA (from Support Protocol) 2X urea RNA loading buffer (see recipe) 10-bp DNA ladder 15%, 10% TBE-urea, 8% TBE polyacrylamide gels SYBR gold (10,000X concentrate) RNase-free water 3 M sodium acetate, pH 5.5 (RNase-free) 25 mg/ml linear polyacrylamide (LPA) Isopropanol 70% (v/v) ethanol 10X T4 polynucleotide kinase (PNK) buffer 10 U/μl T4 PNK Oligonucleotide linker and primers (see Table) 50% (w/v) PEG 8000 (RNase-free) 10X T4 RNA ligase reaction buffer (NEB, B0216L) 200 U/μl T4 RNA ligase 2, truncated (NEB) 10 mM Tris, pH 8.0 (RNase-free) 5X First-strand (FS) RT reaction buffer (Invitrogen, 18080-993) 10 mM dNTPs 0.1 M DTT 20 U/μl SUPERase-In 200 U/μl Superscript III 1 N sodium hydroxide 3 M sodium chloride 10X CircLigase reaction buffer (Epicentre, CL4111K) 1 mM ATP 50 mM manganese chloride 100 U/μl CircLigase ssDNA ligase 5X Phusion HF reaction buffer (NEB, M0530S) 2 U/μl Phusion HF DNA polymerase 10X DNA loading buffer (see recipe) 100-bp DNA ladder DNA gel extraction buffer (see recipe) 10 mM Tris, pH 8.5 Qubit dsDNA HS Assay kit Special equipment: Heat blocks: 80°C, 70°C, 37°C, 25°C Typhoon fluorescent/phosphorescent image scanner, or UV transilluminator with camera Blue light transilluminator (or UV transilluminator) box End over end rotator Costar Spin-X centrifuge tube filter (0.45-um cellulose acetate in 2-ml tube; Corning) Qubit Fluorometer Bioanalyzer DNA high sensitivity chip and reagents Other equipment: Polyacrylamide electrophoresis apparatus and power source 0.5-ml and 1.5-ml RNase-free non-stick microcentrifuge tubes Plastic sheet protector Razor blades or scalpels Thermocycler Plastic sheet protector .. 1 Combine 10 μl RNA from large scale mitoribosome purification with 10 μl 2X urea RNA loading buffer.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs superase in
    Superase In, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in/product/New England Biolabs
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    superase in - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    91
    New England Biolabs superase in rnase inhibitor
    Superase In Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in rnase inhibitor/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superase in rnase inhibitor - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results