superase in  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    New England Biolabs superase in
    Superase In, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in/product/New England Biolabs
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    superase in - by Bioz Stars, 2019-12
    91/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: The end-prepared RNA fragments were ligated to 500 ng of universal miRNA cloning linker (5′-rAppCTGTAGGCACCATCAAT–NH2-3′) (New England Biolabs). .. RNA fragments and linker were heated at 80°C for 2 min and then placed on ice to disrupt secondary structures, and incubated overnight at 16°C in a 20-μL reaction containing 12.5% PEG 8000, 10% DMSO, 20 units SUPERase-In, 200 units T4 RNA Ligase 2 truncated (New England Biolabs), and 1X T4 RNA ligase reaction buffer (NEB).

    RNA Extraction:

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: Total RNA extraction was performed using TRIzol reagent. .. Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Sequencing:

    Article Title: Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA
    Article Snippet: Paragraph title: Global run-on sequencing analysis (GRO-seq) ... First, RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (NEB).

    RNA Sequencing Assay:

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: Paragraph title: RNA sequencing ... RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs).

    Binding Assay:

    Article Title: Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA
    Article Snippet: After binding, beads were washed once in low salt buffer (0.2×SSPE, 1mM EDTA, 0.05% Tween-20), twice in high salt buffer (0.5% SSPE, 1mM EDTA, 0.05% Tween-20, 150mM NaCl), and twice in TET buffer (TE pH7.4, 0.05% Tween-20). .. First, RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (NEB).

    Article Title: A Multi-Parameter Analysis of Cellular Coordination of Major Transcriptome Regulation Mechanisms
    Article Snippet: After binding, beads were washed once in low salt buffer (0.2 × SSPE, 1 mM EDTA, 0.05% Tween-20), twice in high salt buffer (0.5% SSPE, 1 mM EDTA, 0.05% Tween-20, 150 mM NaCl), and twice in TET buffer (TE pH 7.4, 0.05% Tween-20). .. The precipitated RNA was re-suspended in 50 μl reaction (45 μl of DEPC water, 5.2 μl of T4 PNK buffer, 1 μl of SUPERase_In and 1 μl of T4 PNK (NEB)) and incubated at 37 °C for 1 hr.

    Centrifugation:

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. The gel was stained with SYBR Gold (Invitrogen) to visualize the RNA fragments.

    Article Title: Ribosome profiling reveals sequence-independent post-initiation pausing as a signature of translation
    Article Snippet: Purified RNA samples were dephosphorylated in a 15 μl reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. The gel was stained with SYBR Gold (Invitrogen) to visualize the RNA fragments.

    Article Title: Monitoring cotranslational protein folding in mammalian cells at codon resolution
    Article Snippet: The mixed RNA samples were then dephosphorylated in a 15 µL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. The gel was stained with SYBR Gold (Invitrogen) to visualize the RNA fragments.

    Blocking Assay:

    Article Title: Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA
    Article Snippet: Anti-BrdU argarose beads (Santa Cruz Biotech) were blocked in blocking buffer (0.5×SSPE, 1mM EDTA, 0.05% Tween-20, 0.1% PVP, and 1mg/ml BSA) for 1 hr at 4°C. .. The precipitated RNA was resuspended in 50μl reaction (45μl of DEPC water, 5.2μl of T4 PNK buffer, 1μl of SUPERase_In and 1μl of T4 PNK (NEB)) and incubated at 37°C for 1 hr.

    Article Title: A Multi-Parameter Analysis of Cellular Coordination of Major Transcriptome Regulation Mechanisms
    Article Snippet: Anti-BrdU argarose beads (Santa Cruz Biotech) were blocked in blocking buffer (0.5 × SSPE, 1 mM EDTA, 0.05% Tween-20, 0.1% PVP, and 1 mg/ml BSA) for 1 hr at 4 °C. .. The precipitated RNA was re-suspended in 50 μl reaction (45 μl of DEPC water, 5.2 μl of T4 PNK buffer, 1 μl of SUPERase_In and 1 μl of T4 PNK (NEB)) and incubated at 37 °C for 1 hr.

    Size-exclusion Chromatography:

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: The RNA and 5′ linker were incubated at 22°C overnight in a 30-μL reaction containing 10% DMSO, 1X Quick Ligase Buffer (NEB), 20 units SUPERase-In, and 15 units T4 RNA ligase 1 (NEB). .. The RNA and 5′ linker were incubated at 22°C overnight in a 30-μL reaction containing 10% DMSO, 1X Quick Ligase Buffer (NEB), 20 units SUPERase-In, and 15 units T4 RNA ligase 1 (NEB).

    Concentration Assay:

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: RNA fragments were then 5′ phosphorylated by adding 12.5 μL of nuclease free water, 2 μL 10X T4 PNK buffer, 15 units T4 PNK, and ATP to a concentration of 1 mM, and incubating at 37°C for 1 h. The RNA fragments were purified using a RNA Clean & Concentrator-5 column and resuspended in 11.75 μL of nuclease free water. .. RNA fragments and linker were heated at 80°C for 2 min and then placed on ice to disrupt secondary structures, and incubated overnight at 16°C in a 20-μL reaction containing 12.5% PEG 8000, 10% DMSO, 20 units SUPERase-In, 200 units T4 RNA Ligase 2 truncated (New England Biolabs), and 1X T4 RNA ligase reaction buffer (NEB).

    Incubation:

    Article Title: Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA
    Article Snippet: The precipitated RNA was resuspended in 50μl reaction (45μl of DEPC water, 5.2μl of T4 PNK buffer, 1μl of SUPERase_In and 1μl of T4 PNK (NEB)) and incubated at 37°C for 1 hr. .. First, RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (NEB).

    Article Title: Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA
    Article Snippet: RNA was then extracted with acidic phenol/chloroform once, chloroform once and precipitated with ethanol overnight. .. The precipitated RNA was resuspended in 50μl reaction (45μl of DEPC water, 5.2μl of T4 PNK buffer, 1μl of SUPERase_In and 1μl of T4 PNK (NEB)) and incubated at 37°C for 1 hr. .. The RNA was extracted and precipitated again as above. cDNA synthesis was performed basically as described with minor modifications.

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: To convert the polysome into monosome, E. coli RNase I (Ambion) was added into the pooled polysome samples (750 U per 100 A260 units) and incubated at 4 °C for 1 h. SUPERase inhibitor (50 U per 100 U RNase I) was then added to stop digestion. .. Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: A Multi-Parameter Analysis of Cellular Coordination of Major Transcriptome Regulation Mechanisms
    Article Snippet: RNA was then extracted with acidic phenol/chloroform once, chloroform once and precipitated with ethanol overnight. .. The precipitated RNA was re-suspended in 50 μl reaction (45 μl of DEPC water, 5.2 μl of T4 PNK buffer, 1 μl of SUPERase_In and 1 μl of T4 PNK (NEB)) and incubated at 37 °C for 1 hr. .. The RNA was extracted and precipitated again as above before being processed for Illumina NGS sequencing.

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: The end-prepared RNA fragments were ligated to 500 ng of universal miRNA cloning linker (5′-rAppCTGTAGGCACCATCAAT–NH2-3′) (New England Biolabs). .. RNA fragments and linker were heated at 80°C for 2 min and then placed on ice to disrupt secondary structures, and incubated overnight at 16°C in a 20-μL reaction containing 12.5% PEG 8000, 10% DMSO, 20 units SUPERase-In, 200 units T4 RNA Ligase 2 truncated (New England Biolabs), and 1X T4 RNA ligase reaction buffer (NEB). .. The RNA was purified using a RNA Clean & Concentrator-5 column (Zymo Research) using the manufacturer's > 200 nt-long protocol, and resuspended in 10 μL of nuclease free water.

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: To reduce secondary structure, the 5′ linker was incubated at 70°C for 2 min and placed immediately on ice. .. The RNA and 5′ linker were incubated at 22°C overnight in a 30-μL reaction containing 10% DMSO, 1X Quick Ligase Buffer (NEB), 20 units SUPERase-In, and 15 units T4 RNA ligase 1 (NEB). .. The RNA was purified using a RNA Clean & Concentrator-5 column (Zymo Research, > 200-nt protocol) and resuspended in 26 μL of nuclease free water.

    De-Phosphorylation Assay:

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: Dephosphorylation reactions were purified with gel extraction on Novex 10% polyacrylamide TBE–urea gel (Life Technologies). .. RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs).

    Gel Extraction:

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: Dephosphorylation reactions were purified with gel extraction on Novex 10% polyacrylamide TBE–urea gel (Life Technologies). .. RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs).

    cDNA Library Assay:

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: Paragraph title: cDNA library construction of ribosome-protected mRNA fragments ... Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: Ribosome profiling reveals sequence-independent post-initiation pausing as a signature of translation
    Article Snippet: Paragraph title: cDNA library construction of RPF ... Purified RNA samples were dephosphorylated in a 15 μl reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: Monitoring cotranslational protein folding in mammalian cells at codon resolution
    Article Snippet: Paragraph title: cDNA Library Construction of Ribosome-Protected mRNA Fragments. ... The mixed RNA samples were then dephosphorylated in a 15 µL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Staining:

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: Ribosome profiling reveals sequence-independent post-initiation pausing as a signature of translation
    Article Snippet: Purified RNA samples were dephosphorylated in a 15 μl reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. Purified RNA samples were dephosphorylated in a 15 μl reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: Monitoring cotranslational protein folding in mammalian cells at codon resolution
    Article Snippet: The mixed RNA samples were then dephosphorylated in a 15 µL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. The mixed RNA samples were then dephosphorylated in a 15 µL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Purification:

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: Dephosphorylation reactions were purified with gel extraction on Novex 10% polyacrylamide TBE–urea gel (Life Technologies). .. RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs).

    Article Title: Quantitative profiling of initiating ribosomes in vivo
    Article Snippet: Total RNA extraction was performed using TRIzol reagent. .. Purified RNA samples were dephosphorylated in a 15 μL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. Dephosphorylation was carried out for 1 h at 37 °C, and the enzyme was then heat-inactivated for 20 min at 65 °C.

    Article Title: Ribosome profiling reveals sequence-independent post-initiation pausing as a signature of translation
    Article Snippet: Total RNA extraction was performed using TRIzol reagent. .. Purified RNA samples were dephosphorylated in a 15 μl reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB). .. Dephosphorylation was carried out for 1 h at 37 °C, and the enzyme was then heat inactivated for 20 min at 65 °C.

    Article Title: Monitoring cotranslational protein folding in mammalian cells at codon resolution
    Article Snippet: Purified RNA samples were first mixed with 1 nM of synthetic 28-nt random RNA (5′-AUGUACACGGAGUCGACCCGCAACGCGA-3′) as the spike-in control. .. The mixed RNA samples were then dephosphorylated in a 15 µL reaction containing 1× T4 polynucleotide kinase buffer, 10 U SUPERase_In, and 20 U T4 polynucleotide kinase (NEB).

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: RNA fragments were then 5′ phosphorylated by adding 12.5 μL of nuclease free water, 2 μL 10X T4 PNK buffer, 15 units T4 PNK, and ATP to a concentration of 1 mM, and incubating at 37°C for 1 h. The RNA fragments were purified using a RNA Clean & Concentrator-5 column and resuspended in 11.75 μL of nuclease free water. .. RNA fragments and linker were heated at 80°C for 2 min and then placed on ice to disrupt secondary structures, and incubated overnight at 16°C in a 20-μL reaction containing 12.5% PEG 8000, 10% DMSO, 20 units SUPERase-In, 200 units T4 RNA Ligase 2 truncated (New England Biolabs), and 1X T4 RNA ligase reaction buffer (NEB).

    Article Title: Mod-seq: high-throughput sequencing for chemical probing of RNA structure
    Article Snippet: The RNA was purified using a RNA Clean & Concentrator-5 column (Zymo Research) using the manufacturer's > 200 nt-long protocol, and resuspended in 10 μL of nuclease free water. .. The RNA and 5′ linker were incubated at 22°C overnight in a 30-μL reaction containing 10% DMSO, 1X Quick Ligase Buffer (NEB), 20 units SUPERase-In, and 15 units T4 RNA ligase 1 (NEB).

    Chromatin Immunoprecipitation:

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs). .. RNA fragments were subjected to poly-A tailing reaction in 8.0μl volume containing 0.8μl poly-A polymerase buffer, 1μl 1 mM ATP, 0.5μl of SUPERase-In, and 0.75μl poly-A polymerase (New England Biolabs).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    New England Biolabs superase in
    Superase In, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in/product/New England Biolabs
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    superase in - by Bioz Stars, 2019-12
    91/100 stars
      Buy from Supplier

    Image Search Results