superase in rnase inhibitor  (New England Biolabs)


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    Structured Review

    New England Biolabs superase in rnase inhibitor
    Superase In Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in rnase inhibitor/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superase in rnase inhibitor - by Bioz Stars, 2020-08
    91/100 stars

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    Produced:

    Article Title: Detection of Alphacoronavirus vRNA in the Feces of Brazilian Free-Tailed Bats (Tadarida brasiliensis) from a Colony in Florida, USA
    Article Snippet: .. Briefly, cDNA was produced using AccuScript High Fidelity Reverse Transcriptase (Agilent Technologies, Inc., Santa Clara, CA, USA, Cat# 200820) in the presence of SUPERase-In RNase inhibitor, and PCR was performed using Phusion Polymerase (New England BioLabs, Cat# M0530S) with denaturation steps performed at 98 °C. .. The re-amplified samples were individually electrophoresed in a 1.5% ethidium bromide-stained agarose gel and the 440 bp amplicon excised and purified using a Qiagen MinElute Gel Extraction kit (Qiagen, Cat# 28604).

    Polymerase Chain Reaction:

    Article Title: Detection of Alphacoronavirus vRNA in the Feces of Brazilian Free-Tailed Bats (Tadarida brasiliensis) from a Colony in Florida, USA
    Article Snippet: .. Briefly, cDNA was produced using AccuScript High Fidelity Reverse Transcriptase (Agilent Technologies, Inc., Santa Clara, CA, USA, Cat# 200820) in the presence of SUPERase-In RNase inhibitor, and PCR was performed using Phusion Polymerase (New England BioLabs, Cat# M0530S) with denaturation steps performed at 98 °C. .. The re-amplified samples were individually electrophoresed in a 1.5% ethidium bromide-stained agarose gel and the 440 bp amplicon excised and purified using a Qiagen MinElute Gel Extraction kit (Qiagen, Cat# 28604).

    Incubation:

    Article Title: The CRISPR-associated Csx1 protein of Pyrococcus furiosus is an adenosine-specific endoribonuclease
    Article Snippet: .. Products of circular and 3′ end-labeled RNA were treated with 1 U Terminator Exonuclease (TEX; EpiBio), 1× terminator reaction buffer B (EpiBio), and 10 U of SUPERase-IN RNase inhibitor and incubated at 42°C for 30 min. Products of 5′ end-labeled RNA were treated with 5 U E. coli poly(A) polymerase (PAP; NEB), 1× PAP reaction buffer (NEB), and 10 U of SUPERase-IN RNase inhibitor and incubated at 37°C for 20 min. .. Reactions were stopped by placing on ice and adding an equal volume of Gel Loading Buffer II (Life Technologies).

    Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
    Article Snippet: .. For the phosphorylation reaction, samples were denatured at 80 °C for 90 s, equilibrated to 37 °C, and incubated at 37 °C for 1 h with 5 μL of 10× T4 PNK buffer (NEB), 20 U SUPERase-In RNase Inhibitor, and 10 U T4 PNK (NEB). .. After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen).

    Article Title: Transcriptome and translatome profiles of Streptomyces species in different growth phases
    Article Snippet: .. The enriched RPFs were then denatured for 90 s at 80 °C and incubated for 1 h at 37 °C with 5 mL of 10× T4 Polynucleotide Kinase (PNK) buffer (NEB), 20 U of SUPERase-In RNase Inhibitor, and 10 U of T4 PNK (NEB) to dephosphorylate the 3′ end. .. The dephosphorylated RNAs were purified using the RNeasy MinElute Column (Qiagen).

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    New England Biolabs superase in rnase inhibitor
    Superase In Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superase in rnase inhibitor/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superase in rnase inhibitor - by Bioz Stars, 2020-08
    91/100 stars
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