Structured Review

Nacalai super
Super, supplied by Nacalai, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/super/product/Nacalai
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
super - by Bioz Stars, 2020-07
85/100 stars

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Related Articles

Reverse Transcription Polymerase Chain Reaction:

Article Title: Tissue- and Context-Dependent Modulation of Hormonal Sensitivity of Glucocorticoid-Responsive Genes by Hexamethylene Bisacetamide-Inducible Protein 1
Article Snippet: .. Total RNA was prepared with Sepasol-RNA I super (Nacalai Tesque), reverse-transcribed with oligo-dT primer using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen). qRT-PCR was performed with the LightCycler TaqMan Master, Universal ProbeLibrary Set, Human, and LightCycler ST300 systems (Roche, Indianapolis, IN) according to manufacturer’s instructions. .. Expression levels of mRNA were calculated on the basis of standard curves generated for each gene. mRNA for gapdh was used as an internal control.

Quantitative RT-PCR:

Article Title: Tissue- and Context-Dependent Modulation of Hormonal Sensitivity of Glucocorticoid-Responsive Genes by Hexamethylene Bisacetamide-Inducible Protein 1
Article Snippet: .. Total RNA was prepared with Sepasol-RNA I super (Nacalai Tesque), reverse-transcribed with oligo-dT primer using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen). qRT-PCR was performed with the LightCycler TaqMan Master, Universal ProbeLibrary Set, Human, and LightCycler ST300 systems (Roche, Indianapolis, IN) according to manufacturer’s instructions. .. Expression levels of mRNA were calculated on the basis of standard curves generated for each gene. mRNA for gapdh was used as an internal control.

Software:

Article Title: Myostatin Increases Smad2 Phosphorylation and Atrogin-1 Expression in Chick Embryonic Myotubes
Article Snippet: .. Bands were detected by Chemi-Lumi one Super (Nacalai Tesque, Inc., Kyoto, Japan), visualized with the LumiCube (Liponics Inc., Tokyo, Japan), and quantified using the CS Analyzer software (ATTO Co., Tokyo, Japan). .. Anti-Akt (#9272), anti-phospho-Akt (pAkt) (Ser473) (#9271), anti-pAMPKα (Thr172) (#2531), anti-AMPKα (#2532), anti-FOXO1 (#9454), anti-pFOXO1 (Ser 256) (#9461), anti-pSmad2 (Ser465/467) / Smad3 (ser423/425) (#8828), anti-Smad2/3 (#8685), anti-β -actin (#4967), and horseradish peroxidase (HRP) -conjugated anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Article Title: Role of Insulin-like Growth Factor-1 in the Central Regulation of Feeding Behavior in Chicks
Article Snippet: .. Bands were detected using Chemi-Lumi one Super (Nacalai Tesque, Inc., Kyoto, Japan), visualized using LumiCube (Liponics Inc., Tokyo, Japan), and quantified using the CS Analyzer software (ATTO Co., Tokyo, Japan). .. Anti-Akt (#9272), anti-phospho-Akt (pAkt) (Thr308) (#9275), anti-pAkt (Ser473) (#9271), anti-S6 ribosomal protein (S6) (#2217), anti-phospho-S6 ribosomal protein (pS6) (Ser240/244) (#5364), anti-FOXO1 (#9454), anti-phospho-FOXO1 (pFOXO1) (Ser256) (#9461), anti-β -actin (#4967), anti-AMPK (#2532), anti-phospho-AMPK (pAMPK) (Thr172) (#2531), anti-ERK1/2 (#4695), antiphospho-ERK1/2 (#9101) (Thr202/Tyr204) (#4370), anti-α /β -tubulin (#2148) and horseradish peroxidase (HRP) - conjugated anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Beverly, MA, USA).

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