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Promega super rnasein
Super Rnasein, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/super rnasein/product/Promega
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
super rnasein - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

RNA Extraction:

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Paragraph title: RNA extraction and reverse transcriptase PCR (RT-PCR). ... Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions.

Amplification:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction. .. The Janus system of mutant generation is designed to generate unmarked nonpolar mutants; however, to confirm that there were no polar effects, we demonstrated that the mutations had no effect on the transcription of the distal gene by RT-PCR. cDNA was amplified with primers designed within the gene distal to the mutation: primers C.9 and C.10 for the scrT mutant ( scrK ), primers U.9 and U.10 for the susT1T2X mutant ( susH ), and primers H.9 and H.10 for the susH (SP_1794) mutant.

Mutagenesis:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction. .. The Janus system of mutant generation is designed to generate unmarked nonpolar mutants; however, to confirm that there were no polar effects, we demonstrated that the mutations had no effect on the transcription of the distal gene by RT-PCR. cDNA was amplified with primers designed within the gene distal to the mutation: primers C.9 and C.10 for the scrT mutant ( scrK ), primers U.9 and U.10 for the susT1T2X mutant ( susH ), and primers H.9 and H.10 for the susH (SP_1794) mutant.

Incubation:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: .. Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase (RT) according to the manufacturer's recommendations (Clontech, Mountain View, CA).

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: .. Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase according to the manufacturer's recommendations (New England BioLabs).

Article Title: Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence
Article Snippet: .. Nucleic acid was incubated with 5 μL DNase I buffer, 3 μL DNase I (New England Biolabs) and 1 μL Super RNaseIN (Promega) at 37°C for 1 hr. .. Cleanup of RNA was performed with a Qiagen RNeasy mini kit as per manufacturer’s instructions.

Quantitative RT-PCR:

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions. .. Quantitative real-time RT-PCR (qRT-PCR) was conducted to measure gene expression profiles under various growth conditions.

Purification:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: .. Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase (RT) according to the manufacturer's recommendations (Clontech, Mountain View, CA).

SYBR Green Assay:

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions. .. The brilliant SYBR green qRT-PCR Master Mix (Stratagene) was used according to the manufacturer's instructions.

Reverse Transcription Polymerase Chain Reaction:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: Paragraph title: RNA preparation and RT-PCR. ... Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction.

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Paragraph title: RNA extraction and reverse transcriptase PCR (RT-PCR). ... Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions.

Article Title: Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence
Article Snippet: Paragraph title: RNA preparation and reverse transcriptase RT-PCR ... Nucleic acid was incubated with 5 μL DNase I buffer, 3 μL DNase I (New England Biolabs) and 1 μL Super RNaseIN (Promega) at 37°C for 1 hr.

Generated:

Article Title: The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides
Article Snippet: Nucleic acid was incubated with 5 μl of 10× DNase I buffer, 6 U DNase I (New England BioLabs), and 20 U Super RNaseIN (Promega, Madison, WI) at 37°C for 1 h. Purification of RNA was performed with a Qiagen RNeasy minikit (Valencia, CA) as per the manufacturer's instruction. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase (RT) according to the manufacturer's recommendations (Clontech, Mountain View, CA).

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase according to the manufacturer's recommendations (New England BioLabs).

Article Title: Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence
Article Snippet: Nucleic acid was incubated with 5 μL DNase I buffer, 3 μL DNase I (New England Biolabs) and 1 μL Super RNaseIN (Promega) at 37°C for 1 hr. .. DNase- and RNase-free reagents were used throughout. cDNA was generated with SmartScribe reverse transcriptase according to the manufacturer’s recommendations (New England Biolabs).

Expressing:

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions. .. Quantitative real-time RT-PCR (qRT-PCR) was conducted to measure gene expression profiles under various growth conditions.

Polymerase Chain Reaction:

Article Title: Identification of an ATPase, MsmK, Which Energizes Multiple Carbohydrate ABC Transporters in Streptococcus pneumoniae ▿
Article Snippet: Paragraph title: RNA extraction and reverse transcriptase PCR (RT-PCR). ... Nucleic acid was incubated with 5 μl DNase I buffer, 3 μl DNase I (New England BioLabs), and 1 μl Super RNaseIN (Promega) at 37°C for 1 h. Cleanup of RNA was performed with a Qiagen RNeasy minikit as per the manufacturer's instructions.

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    Promega rnasin super
    Rnasin Super, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasin super/product/Promega
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rnasin super - by Bioz Stars, 2020-04
    86/100 stars
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