sulfolobus dna polymerase iv  (New England Biolabs)


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    Name:
    Sulfolobus DNA Polymerase IV Lesion Bypass Polymerase
    Description:
    Sulfolobus DNA Polymerase IV Lesion Bypass Polymerase 100 units
    Catalog Number:
    m0327s
    Price:
    121
    Size:
    100 units
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs sulfolobus dna polymerase iv
    Sulfolobus DNA Polymerase IV Lesion Bypass Polymerase
    Sulfolobus DNA Polymerase IV Lesion Bypass Polymerase 100 units
    https://www.bioz.com/result/sulfolobus dna polymerase iv/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sulfolobus dna polymerase iv - by Bioz Stars, 2020-11
    95/100 stars

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    Purification:

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. Two protocols were used for translesion synthesis: In our initial protocol (used for experiments in Figures 1, 3, 4, 5, and 6), ten 100 µl PCRs were individually purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol, eluted in 30 µl of nuclease-free water each, and combined with 100 µl of Thermo Pol Buffer, 20 µl of 10 mM dNTPs, 10 µl of Sulfolobus DNA polymerase IV (New England Biolabs) and nuclease-free water to 1 ml. .. The 1 ml master mix was split into 100 µl aliquots and incubated at 55°C for 1 hour.

    Incubation:

    Article Title: A framework for exhaustively mapping functional missense variants
    Article Snippet: .. A 30 μl reaction containing 1× Taq DNA ligase buffer, 0.2 mM dNTPs, 2 U Sulfolobus DNA polymerase IV (NEB), and 40 U Taq DNA ligase (NEB) was added to the DNA and was incubated at 37°C for 2 h. .. Degradation of wild‐type template 1 μl fill‐in reaction was added to a 20 μl reaction containing 1× UDG buffer and 5 U Uracil‐DNA glycosylase (NEB) and incubated at 37°C for 2 h. Amplification of mutagenized DNA.

    Article Title: Related haloarchaeal pleomorphic viruses contain different genome types
    Article Snippet: .. HRPV-3 and HGPV-1 genomic DNA were incubated with Sulfolobus DNA polymerase IV (2 U/1 µg of DNA; New England Biolabs) in a reaction containing 1× enzyme buffer and 0.4 mM dNTP. .. Reactions were carried out at +37 °C for 3 h. Ligation was done with T4 DNA ligase (Fermentas) as described by manufacturer.

    Article Title: Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
    Article Snippet: .. Adaptor 2 annealing was then carried out by adding 2 μL of 10× Ampligase Reaction Buffer (Epicentre-Illumina), 2 μL 10× dNTPs (2.5 mM each; Invitrogen), and 2 μL 10 μM Tn5mC-A2top (5′-/5Phos/ CTGTCTCTTATACACATCT [5mC] TGAG [5mC] GGG [5mC] TGG [5mC] AAGG [5mC] AGA [5mC] [5mC] GAT [5mC]-3′; IDT) to each reaction and incubating for 2 min at 50°C followed by 10 min at 45°C and cooling at 0.1°C/sec to 37°C and subsequent incubation for 10 min. Gap-repair was then performed by adding 3 μL of Ampligase at 5U/μL (Epicentre-Illumina) and 1 μL of either T4 DNA Polymerase (Tn5mC libraries A-G, NEB) or Sulfolobus DNA Polymerase IV (Tn5mC libraries H-J, NEB) and additional incubation for 30 min at 37°C. .. Reactions were then cleaned up using SPRI beads (AMPure) according to recommended protocol using 36 μL beads and elution in 50 μL nuclease-free water (Ambion).

    other:

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: All 10 µl primer extension reactions contained 5 nM endlabeled primer, 7.5 nM template oligonucleotide (pre-annealed), 1X Thermo Pol Buffer, and 0.02 U/µl Sulfolobus DNA polymerase IV.

    DNA Purification:

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. We later determined that, because Vent Exo- and Dpo4 use the same buffer and dNTP conditions, DNA purification after PCR was unnecessary and efficient translesion synthesis could be achieved by pooling the initial 100 µl PCRs, adding 1 µl of Sulfolobus DNA polymerase IV per 100 µl reaction volume, splitting the reaction master mix into 100 µl aliquots, and incubating at 55°C for 1 hour; this protocol was used to prepare DNA templates for Figure 7 and Figure S2A. .. For both protocols, the 1 mL translesion synthesis master mix contained ~4.5 µg of the truncated PCR product.

    Translesion Synthesis:

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. We later determined that, because Vent Exo- and Dpo4 use the same buffer and dNTP conditions, DNA purification after PCR was unnecessary and efficient translesion synthesis could be achieved by pooling the initial 100 µl PCRs, adding 1 µl of Sulfolobus DNA polymerase IV per 100 µl reaction volume, splitting the reaction master mix into 100 µl aliquots, and incubating at 55°C for 1 hour; this protocol was used to prepare DNA templates for Figure 7 and Figure S2A. .. For both protocols, the 1 mL translesion synthesis master mix contained ~4.5 µg of the truncated PCR product.

    Article Title: Chemical transcription roadblocking for nascent RNA display
    Article Snippet: .. Translesion synthesis reactions were prepared by combining the eluted DNA with 100 μl of Thermo Pol Buffer (New England Biolabs), 20 μl of 10 mM dNTPs (New England Biolabs), 10 μl of Sulfolobus DNA polymerase IV (New England Biolabs) and nuclease-free water to 1 ml. .. The 1 ml master mix was split into 100 μl aliquots and incubated at 55C for 1 hour.

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. Two protocols were used for translesion synthesis: In our initial protocol (used for experiments in Figures 1, 3, 4, 5, and 6), ten 100 µl PCRs were individually purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol, eluted in 30 µl of nuclease-free water each, and combined with 100 µl of Thermo Pol Buffer, 20 µl of 10 mM dNTPs, 10 µl of Sulfolobus DNA polymerase IV (New England Biolabs) and nuclease-free water to 1 ml. .. The 1 ml master mix was split into 100 µl aliquots and incubated at 55°C for 1 hour.

    Polymerase Chain Reaction:

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. We later determined that, because Vent Exo- and Dpo4 use the same buffer and dNTP conditions, DNA purification after PCR was unnecessary and efficient translesion synthesis could be achieved by pooling the initial 100 µl PCRs, adding 1 µl of Sulfolobus DNA polymerase IV per 100 µl reaction volume, splitting the reaction master mix into 100 µl aliquots, and incubating at 55°C for 1 hour; this protocol was used to prepare DNA templates for Figure 7 and Figure S2A. .. For both protocols, the 1 mL translesion synthesis master mix contained ~4.5 µg of the truncated PCR product.

    Article Title: Chemical roadblocking of DNA transcription for nascent RNA display.
    Article Snippet: .. Two protocols were used for translesion synthesis: In our initial protocol (used for experiments in Figures 1, 3, 4, 5, and 6), ten 100 µl PCRs were individually purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol, eluted in 30 µl of nuclease-free water each, and combined with 100 µl of Thermo Pol Buffer, 20 µl of 10 mM dNTPs, 10 µl of Sulfolobus DNA polymerase IV (New England Biolabs) and nuclease-free water to 1 ml. .. The 1 ml master mix was split into 100 µl aliquots and incubated at 55°C for 1 hour.

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  • 95
    New England Biolabs sulfolobus dna polymerase iv
    Sulfolobus Dna Polymerase Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sulfolobus dna polymerase iv/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sulfolobus dna polymerase iv - by Bioz Stars, 2020-11
    95/100 stars
      Buy from Supplier

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