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1) Product Images from "Adeno-associated virus serotype 2 induces cell-mediated immune responses directed against multiple epitopes of the capsid protein VP1"
Article Title: Adeno-associated virus serotype 2 induces cell-mediated immune responses directed against multiple epitopes of the capsid protein VP1
Journal: The Journal of General Virology
Figure Legend Snippet: Serological response to AAV-2 in Irish blood donors. (a) AAV-2-specific total IgG in human donor plasma ( n =45). Specific IgG was determined by indirect AAV-2 antigen ELISA, normalized by comparison with standard positive and negative samples. The negative cut-off (dashed line) was defined at 2 sd above the concentration of a known seronegative standard. (b–e) AAV-2-specific IgG subclasses present in IgG-positive samples ( n =41). IgG1(b), IgG2 (c), IgG3 (d) and IgG4 (e) determined by AAV-2-specific direct antigen ELISA for triplicate samples and quantified by comparison with known reference sera.
Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay