Journal: Journal of infection in developing countries
Article Title: Development and performance evaluation of a multiplex fluorescent PCR method for the detection of fastidious pathogens causing respiratory infections in children.
doi: 10.3855/jidc.20282
Figure Lengend Snippet: Figure 4. The AUC plots of different methods for the detection of fastidious respiratory bacteria: Figures A, C, E, and G show the AUC of HI, SPN, MC, and NM detected by culture method, respectively. Figure B shows the AUC of HI detected by the singleplex fluorescent method. Figures D, F, H, I, and J show the AUC of SPN, MC, NM, BP, and LP detected by the multiplex fluorescent PCR method.
Article Snippet: Specificity test: The specificity of singleplex and multiple fluorescent PCR was evaluated using a variety of bacteria, including the standard strains HI (ATCC49247), MC (ATCC25238), NM (ATCC13090), and SPN (ATCC49619), LP (ATCC33152), BP (ATCC9340), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus (ATCC 25923).
Techniques: Bacteria, Multiplex Assay