streptavidin  (Kingfisher Biotech)


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    Structured Review

    Kingfisher Biotech streptavidin
    <t>Streptavidin</t> and His-tag pull down assay of CFPS malE-en2NTS 1 and E. coli expressed en2NTS 1 . Binding assay, testing capability of binding to Biotin-NT using Streptavidin dynabeads in combination with ( A ). CFPS produced malE-en2NTS 1 and ( B ). The E. coli expressed en2NTS 1 control. Each graph reads 1. Receptor alone. 2. Biotin-NT plus receptor. 3. Biotin NT, receptor and unlabelled NT 8–13 competitor. Binding assay using His-tag isolation dynabeads in combination with ( C ). CFPS produced malE-en2NTS 1 ( D ). E. coli expressed en2NTS 1 . Each graph reads 1. Receptor alone. 2. A647-NT 8–13 plus receptor. 3. A647-NT 8–13 , receptor and NT 8–13 competitor. Data is presented as triplicate experiments ± SEM.
    Streptavidin, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin/product/Kingfisher Biotech
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin - by Bioz Stars, 2022-08
    86/100 stars

    Images

    1) Product Images from "Characterisation of a cell-free synthesised G-protein coupled receptor"

    Article Title: Characterisation of a cell-free synthesised G-protein coupled receptor

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01227-z

    Streptavidin and His-tag pull down assay of CFPS malE-en2NTS 1 and E. coli expressed en2NTS 1 . Binding assay, testing capability of binding to Biotin-NT using Streptavidin dynabeads in combination with ( A ). CFPS produced malE-en2NTS 1 and ( B ). The E. coli expressed en2NTS 1 control. Each graph reads 1. Receptor alone. 2. Biotin-NT plus receptor. 3. Biotin NT, receptor and unlabelled NT 8–13 competitor. Binding assay using His-tag isolation dynabeads in combination with ( C ). CFPS produced malE-en2NTS 1 ( D ). E. coli expressed en2NTS 1 . Each graph reads 1. Receptor alone. 2. A647-NT 8–13 plus receptor. 3. A647-NT 8–13 , receptor and NT 8–13 competitor. Data is presented as triplicate experiments ± SEM.
    Figure Legend Snippet: Streptavidin and His-tag pull down assay of CFPS malE-en2NTS 1 and E. coli expressed en2NTS 1 . Binding assay, testing capability of binding to Biotin-NT using Streptavidin dynabeads in combination with ( A ). CFPS produced malE-en2NTS 1 and ( B ). The E. coli expressed en2NTS 1 control. Each graph reads 1. Receptor alone. 2. Biotin-NT plus receptor. 3. Biotin NT, receptor and unlabelled NT 8–13 competitor. Binding assay using His-tag isolation dynabeads in combination with ( C ). CFPS produced malE-en2NTS 1 ( D ). E. coli expressed en2NTS 1 . Each graph reads 1. Receptor alone. 2. A647-NT 8–13 plus receptor. 3. A647-NT 8–13 , receptor and NT 8–13 competitor. Data is presented as triplicate experiments ± SEM.

    Techniques Used: Pull Down Assay, Binding Assay, Produced, Isolation

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