streptavidin poly hrp conjugate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin poly hrp conjugate
    Streptavidin Poly Hrp Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin poly hrp conjugate/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    streptavidin poly hrp conjugate - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: 2.5 ELISA for ABD screening Cell lysates of clones of E. coli BL21 BirA host cells producing biotinylated protein variants were prepared using lysozyme solution (PBS buffer, 0·05% Tween, 1% lysozyme, 25 U/mL benzonase, pH 7·4) or using sonicator (Misonix 3000). .. The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce).

    Negative Control:

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: .. The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce). .. The V5-tagged gp120 recombinant protein was diluted in PBSTB and detected by anti-V5 tag – HRP conjugate in PBSTB (1:10,000).

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: ATD samples cleaved at the stated timepoints were added at 5 μg/mL and supernatant from un-cleaved cells was added as negative control, followed by 1 h incubation at 37 °C. .. After three additional PBST washes, Streptavidin-poly-HRP conjugate at 1:2000 (Thermo Fisher) was added and incubated for 1 h at 37 °C.

    Recombinant:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific). .. Glycan blot-ting was also used to determine affinity of increasing concentrations (0.5 and 1.5 lg) of recombinant mouse lactadherin protein (MFG-E8; R & D Systems) to biotinylated suLeX , LeX and N -acetyllactosamine (LN).

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce). .. The V5-tagged gp120 recombinant protein was diluted in PBSTB and detected by anti-V5 tag – HRP conjugate in PBSTB (1:10,000).

    Blocking Assay:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: .. After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific). .. Glycan blot-ting was also used to determine affinity of increasing concentrations (0.5 and 1.5 lg) of recombinant mouse lactadherin protein (MFG-E8; R & D Systems) to biotinylated suLeX , LeX and N -acetyllactosamine (LN).

    Purification:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: Paragraph title: Purification and identification of sulfated Lewis X-binding proteins from epididymal spermatozoa ... After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific).

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: .. The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce). .. The V5-tagged gp120 recombinant protein was diluted in PBSTB and detected by anti-V5 tag – HRP conjugate in PBSTB (1:10,000).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: Paragraph title: ELISA for ABD screening ... The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce).

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: To test binding of TEV-mobilized ATD to plate-adherent human IgG by ELISA, we first biotinylated the ATD using the EZ-Link™ Sulfo-NHS-Biotin kit (21,326, Thermo Fisher). .. After three PBST washes, Streptavidin-poly-HRP conjugate at 1:2000 (Thermo Fisher) was added and incubated for 1 h at 37 °C.

    Article Title: RBC Adherence of Immune Complexes Containing Botulinum Toxin Improves Neutralization and Macrophage Uptake
    Article Snippet: Streptavidin-poly-horseradish peroxidase conjugate (Thermo Fisher Scientific, Waltham, MA, USA) was added (1:2000), incubated for 1 h at 37 °C, and washed 3× with PBST. .. Super Signal ELISA Femto Substrate (Thermo Fisher Scientific, Waltham, MA, USA) was used for detection and relative luminescence values were measured using Biotek Synergy II Microplate reader (BioTek Instruments, Winooski, VT, USA).

    Article Title: Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
    Article Snippet: Paragraph title: Competition ELISA ... This mixture was then transferred to an assay plate, and the plate incubated for 1 h. The plate was washed with wash buffer, and presence of bound IL-6 detected through addition of streptavidin poly-horseradish peroxidase conjugate (Pierce, Rockford IL).

    Concentration Assay:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: Aliquots (21–22 µL) from the collected RP-HPLC fractions were diluted in 4× LSB (final concentration: 10% glycerol, 50 mM Tris, 2% sodium dodecyl sulfate, 100 mM DTT, 0.025% Bromophenol Blue) and heated at 100 °C for 7 min. Proteins were resolved by SDS-PAGE on 8.6 × 6.7 cm 4–20% gradient gels followed by transfer onto a nitrocellulose membrane. .. After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific).

    Incubation:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: .. After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific). .. Glycan blot-ting was also used to determine affinity of increasing concentrations (0.5 and 1.5 lg) of recombinant mouse lactadherin protein (MFG-E8; R & D Systems) to biotinylated suLeX , LeX and N -acetyllactosamine (LN).

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: .. After three PBST washes, Streptavidin-poly-HRP conjugate at 1:2000 (Thermo Fisher) was added and incubated for 1 h at 37 °C. ..

    Article Title: RBC Adherence of Immune Complexes Containing Botulinum Toxin Improves Neutralization and Macrophage Uptake
    Article Snippet: .. Streptavidin-poly-horseradish peroxidase conjugate (Thermo Fisher Scientific, Waltham, MA, USA) was added (1:2000), incubated for 1 h at 37 °C, and washed 3× with PBST. .. Super Signal ELISA Femto Substrate (Thermo Fisher Scientific, Waltham, MA, USA) was used for detection and relative luminescence values were measured using Biotek Synergy II Microplate reader (BioTek Instruments, Winooski, VT, USA).

    Article Title: Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
    Article Snippet: .. This mixture was then transferred to an assay plate, and the plate incubated for 1 h. The plate was washed with wash buffer, and presence of bound IL-6 detected through addition of streptavidin poly-horseradish peroxidase conjugate (Pierce, Rockford IL). .. Plates were developed using a TMB substrate kit (Pierce) and stopped with 2N H2 SO4 .

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: .. After three additional PBST washes, Streptavidin-poly-HRP conjugate at 1:2000 (Thermo Fisher) was added and incubated for 1 h at 37 °C. ..

    Inhibition:

    Article Title: Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
    Article Snippet: This mixture was then transferred to an assay plate, and the plate incubated for 1 h. The plate was washed with wash buffer, and presence of bound IL-6 detected through addition of streptavidin poly-horseradish peroxidase conjugate (Pierce, Rockford IL). .. Inhibition of complex formation was measured as a reduction in absorbance at 450 nm.

    Competitive ELISA:

    Article Title: Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
    Article Snippet: Competition ELISA Peptides were initially evaluated using a competitive enzyme-linked immunosorbent assay (ELISA). .. This mixture was then transferred to an assay plate, and the plate incubated for 1 h. The plate was washed with wash buffer, and presence of bound IL-6 detected through addition of streptavidin poly-horseradish peroxidase conjugate (Pierce, Rockford IL).

    Western Blot:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: .. After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific). .. Glycan blot-ting was also used to determine affinity of increasing concentrations (0.5 and 1.5 lg) of recombinant mouse lactadherin protein (MFG-E8; R & D Systems) to biotinylated suLeX , LeX and N -acetyllactosamine (LN).

    Binding Assay:

    Article Title: Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
    Article Snippet: .. The lysate samples (diluted 33×), purified protein variants as well as wild type ABD (ABDwt), serving as a negative control, diluted in PBSTB were applied and their binding was detected using streptavidin Poly-HRP conjugate diluted in PBSTB 1:10,000 (Pierce). .. The V5-tagged gp120 recombinant protein was diluted in PBSTB and detected by anti-V5 tag – HRP conjugate in PBSTB (1:10,000).

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: To test binding of TEV-mobilized ATD to plate-adherent human IgG by ELISA, we first biotinylated the ATD using the EZ-Link™ Sulfo-NHS-Biotin kit (21,326, Thermo Fisher). .. After three PBST washes, Streptavidin-poly-HRP conjugate at 1:2000 (Thermo Fisher) was added and incubated for 1 h at 37 °C.

    Article Title: RBC Adherence of Immune Complexes Containing Botulinum Toxin Improves Neutralization and Macrophage Uptake
    Article Snippet: Paragraph title: 5.3. Monoclonal Antibody Competitive Binding Studies ... Streptavidin-poly-horseradish peroxidase conjugate (Thermo Fisher Scientific, Waltham, MA, USA) was added (1:2000), incubated for 1 h at 37 °C, and washed 3× with PBST.

    Article Title: Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
    Article Snippet: High binding 96-well microtiter plates (half-area, Corning Life Sciences, Corning, NY)) were coated with 0.1 μg per well of IL-6R (PeproTech, Rocky Hill, NJ) overnight at 4°C. .. This mixture was then transferred to an assay plate, and the plate incubated for 1 h. The plate was washed with wash buffer, and presence of bound IL-6 detected through addition of streptavidin poly-horseradish peroxidase conjugate (Pierce, Rockford IL).

    SDS Page:

    Article Title: Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine spermatozoa
    Article Snippet: Aliquots (21–22 µL) from the collected RP-HPLC fractions were diluted in 4× LSB (final concentration: 10% glycerol, 50 mM Tris, 2% sodium dodecyl sulfate, 100 mM DTT, 0.025% Bromophenol Blue) and heated at 100 °C for 7 min. Proteins were resolved by SDS-PAGE on 8.6 × 6.7 cm 4–20% gradient gels followed by transfer onto a nitrocellulose membrane. .. After glycan incubation and rinsing, bound suLeX was detected by incubation with Streptavidin Poly-HRP Conjugate (Pierce Biotechnology, Rockford, IL, USA), diluted 1 : 60,000 in blocking buffer for 30 min, and developed using ECL Western Blotting Substrate (Thermo Scientific).

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher poly hrp conjugated streptavidin
    Poly Hrp Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly hrp conjugated streptavidin/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly hrp conjugated streptavidin - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin poly hrp conjugate
    Streptavidin Poly Hrp Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin poly hrp conjugate/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    streptavidin poly hrp conjugate - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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