Structured Review

Agilent technologies streptavidin horseradish
Streptavidin Horseradish, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin horseradish/product/Agilent technologies
Average 86 stars, based on 2 article reviews
Price from $9.99 to $1999.99
streptavidin horseradish - by Bioz Stars, 2020-02
86/100 stars

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Immunohistochemistry:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Paragraph title: Immunohistochemistry ... The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min).

Immunodetection:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Immunodetection of Ki67, cleaved Caspase-3 and erbB2 was performed on the NexES stainer (Ventana Medical Systems, Tucson, AZ) at an operating temperature of 37°C. .. The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min).

Blocking Assay:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min). .. Sections were sequentially blocked for 10 min in 3% hydrogen peroxide (v/v) and 30 min in Rodent Block M (Biocare, cat# RBM961), followed by primary antibody incubation for 30 min and 30 min in polymer.

Incubation:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit. .. The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min).

Modification:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit. .. The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min).

Plasmid Preparation:

Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Article Snippet: The I-VIEW secondary antibody and enzyme were replaced with a species specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 min) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA; 8 min). .. ErbB3 was incubated for 32 min and detected with a modified I-VIEW DAB kit in which the secondary antibody was replaced with Rabbit ImmPress (Vector Labs; Burlingame, CA; cat# MP-7401; 8 minutes at 37°C) and enzyme was replaced with Rabbit ImmPress (Diluted 1:1 in PBS pH 7.6; 8 minutes at 37°C).

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