streptavidin horseradish peroxidase solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin horseradish peroxidase solution
    Streptavidin Horseradish Peroxidase Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horseradish peroxidase solution/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin horseradish peroxidase solution - by Bioz Stars, 2020-04
    91/100 stars

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    TUNEL Assay:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: Subsequently, 250 μl TUNEL reaction mixture containing biotinylated nucleotide and TdT was added to sections of the positive and treatment groups for 60 min at 37 C in the dark. .. The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide.

    Electron Microscopy:

    Article Title: Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism
    Article Snippet: .. Slides were developed with streptavidin-horseradish peroxidase solution (Life Technologies, ThermoFisher Scientific, Burlington, ON, Canada), counterstained with hematoxylin (Life Technologies), and finally mounted with CLEAR-MOUNT Tris buffer solution (Electron Microscopy Sciences, Hatfield, PA, USA). .. Macrophages stained with CD68 were quantified using the software Image Pro-Plus (Media Cybernetics, Rockville, MD, USA).

    Immunohistochemistry:

    Article Title: Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism
    Article Snippet: Paragraph title: PAS staining and Immunohistochemistry (IHC) ... Slides were developed with streptavidin-horseradish peroxidase solution (Life Technologies, ThermoFisher Scientific, Burlington, ON, Canada), counterstained with hematoxylin (Life Technologies), and finally mounted with CLEAR-MOUNT Tris buffer solution (Electron Microscopy Sciences, Hatfield, PA, USA).

    Article Title: Retinol Improves In Vitro Differentiation of Pre-Pubertal Mouse Spermatogonial Stem Cells into Sperm during the First Wave of Spermatogenesis
    Article Snippet: Paragraph title: Immunohistochemistry ... Specific staining was achieved by incubation with a streptavidin-horseradish peroxidase solution (HRP, Thermo Scientific) for 15 minutes followed by an incubation with 3,3′-diaminobenzidine substrate (DAB, Thermo scientific) for 1–10 minutes.

    Positive Control:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: At the beginning of the experiment, the positive control sections were incubated with DNase (RQ1 RNase-free DNase from Promega; catalog no. M6101) for 5 min and rinsed in water. .. The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide.

    Transferring:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: The reaction was terminated by transferring all the slides to the termination buffer for 15 min. .. The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide.

    Negative Control:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: For negative control, TdT was omitted from the reaction mixture. .. The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide.

    Microscopy:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide. .. The sections were counterstained with hematoxylin and photographed in an ECLIPSE 80i Nikon microscope connected to a computer with ACT-2U program for the DS-5M camera.

    Incubation:

    Article Title: Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism
    Article Snippet: Incubation with anti-CD68 primary antibody (1:100 dilutions, Abcam, Cambridge, MA, USA) or anti-collagen 1 primary antibody (1:200 dilutions, Abbiotec, San Diego, CA, USA) was conducted overnight at 4 °C, followed the next day by a 1-h incubation with the secondary antibody, biotin-labeled goat anti–mouse Ig (1:100, BD Biosciences, San Jose, CA, USA). .. Slides were developed with streptavidin-horseradish peroxidase solution (Life Technologies, ThermoFisher Scientific, Burlington, ON, Canada), counterstained with hematoxylin (Life Technologies), and finally mounted with CLEAR-MOUNT Tris buffer solution (Electron Microscopy Sciences, Hatfield, PA, USA).

    Article Title: Mimotopic peptide immunotherapy for the treatment of multiple sclerosis, an inflammatory autoimmune disease
    Article Snippet: .. The assay encompassed the following steps: (a) the linker-spiked test serum sample used in the specific IgE assay was diluted 1/25,000 by first making a 1/100 dilution by mixing together 100 μL of linker-spiked serum and 9.9 mL of PBST; and then spiking 11.950 mL of HSA conjugate diluent with 48 μL of the 1/100 diluted serum; (b) test plates were filled with 100 μL/well of diluted serum, sealed, and incubated for 2 h at 21-26°C; (c) equal volumes of biotinylated goat anti-human kappa antibody (Vector Laboratories, BA3060) and biotinylated, goat anti-human lambda antibody (Vector Laboratories, BA-3070) were mixed together to form a 50:50 biotinylated anti-(kappa + lambda) concentrate (500 μg/mL ); (d) 96 μL of the anti-(kappa + lambda) concentrate was mixed with 11.9 mL of HSA conjugate diluent, and a 100 μL aliquot of this solution was applied to each well; (e) after a 2 h incubation at 21-26°C, plates were washed and 100 μL of 16 ng/mL streptavidin horseradish peroxidase solution was applied to each well; (f) test plates were incubated at 21-26°C for 30 min, aspirated, and washed; and (g) 100 μL/well of Thermo Scientific chemiluminescence substrate was applied and the plate wells were read at 1-3 min post-application in a microplate lumin-ometer. ..

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: .. The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide. .. The sections were counterstained with hematoxylin and photographed in an ECLIPSE 80i Nikon microscope connected to a computer with ACT-2U program for the DS-5M camera.

    Article Title: Retinol Improves In Vitro Differentiation of Pre-Pubertal Mouse Spermatogonial Stem Cells into Sperm during the First Wave of Spermatogenesis
    Article Snippet: .. Specific staining was achieved by incubation with a streptavidin-horseradish peroxidase solution (HRP, Thermo Scientific) for 15 minutes followed by an incubation with 3,3′-diaminobenzidine substrate (DAB, Thermo scientific) for 1–10 minutes. .. Finally, the nuclei were counterstained with haematoxylin (Dako).

    Cell Culture:

    Article Title: Retinol Improves In Vitro Differentiation of Pre-Pubertal Mouse Spermatogonial Stem Cells into Sperm during the First Wave of Spermatogenesis
    Article Snippet: Specific staining was achieved by incubation with a streptavidin-horseradish peroxidase solution (HRP, Thermo Scientific) for 15 minutes followed by an incubation with 3,3′-diaminobenzidine substrate (DAB, Thermo scientific) for 1–10 minutes. .. Furthermore, the spermatozoa present in cell suspensions obtained from cultured testicular fragments were identified by immunofluorescent staining of their flagella using a monoclonal mouse anti-acetylated tubulin antibody (T6793, 1:100, Sigma) (see “ ” below).

    Staining:

    Article Title: Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism
    Article Snippet: Paragraph title: PAS staining and Immunohistochemistry (IHC) ... Slides were developed with streptavidin-horseradish peroxidase solution (Life Technologies, ThermoFisher Scientific, Burlington, ON, Canada), counterstained with hematoxylin (Life Technologies), and finally mounted with CLEAR-MOUNT Tris buffer solution (Electron Microscopy Sciences, Hatfield, PA, USA).

    Article Title: Retinol Improves In Vitro Differentiation of Pre-Pubertal Mouse Spermatogonial Stem Cells into Sperm during the First Wave of Spermatogenesis
    Article Snippet: .. Specific staining was achieved by incubation with a streptavidin-horseradish peroxidase solution (HRP, Thermo Scientific) for 15 minutes followed by an incubation with 3,3′-diaminobenzidine substrate (DAB, Thermo scientific) for 1–10 minutes. .. Finally, the nuclei were counterstained with haematoxylin (Dako).

    Activated Clotting Time Assay:

    Article Title: Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters That Show Progesterone-Dependent Implantation
    Article Snippet: The sections were rinsed in PBS, incubated in 0.3% (vol/vol) hydrogen peroxide (H2 O2 ), washed in PBS, and incubated with streptavidin horseradish peroxidase solution for 60 min. After the sections were washed in PBS, aminoethylcarbazole (AEC) single solution (Zymed Laboratories Inc., San Francisco, CA; catalog no. 00-1111) was added to the slide and developed color to detect biotinylated nucleotide. .. The sections were counterstained with hematoxylin and photographed in an ECLIPSE 80i Nikon microscope connected to a computer with ACT-2U program for the DS-5M camera.

    Software:

    Article Title: Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism
    Article Snippet: Slides were developed with streptavidin-horseradish peroxidase solution (Life Technologies, ThermoFisher Scientific, Burlington, ON, Canada), counterstained with hematoxylin (Life Technologies), and finally mounted with CLEAR-MOUNT Tris buffer solution (Electron Microscopy Sciences, Hatfield, PA, USA). .. Macrophages stained with CD68 were quantified using the software Image Pro-Plus (Media Cybernetics, Rockville, MD, USA).

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    Thermo Fisher elispot kit strepavidin hrp solution
    Elispot Kit Strepavidin Hrp Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elispot kit strepavidin hrp solution/product/Thermo Fisher
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    Thermo Fisher streptavidin hrp conjugate solution
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    https://www.bioz.com/result/streptavidin hrp conjugate solution/product/Thermo Fisher
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Thermo Fisher streptavidin horseradish peroxidase hrp solution
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    https://www.bioz.com/result/streptavidin horseradish peroxidase hrp solution/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin horseradish peroxidase hrp solution - by Bioz Stars, 2020-04
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    Thermo Fisher sensitivity streptavidin conjugated hrp enzymes
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