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Rockland Immunochemicals plasma incubation
Plasma Incubation, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasma incubation/product/Rockland Immunochemicals
Average 92 stars, based on 94 article reviews
Price from $9.99 to $1999.99
plasma incubation - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Amplification:

Article Title: Effective fiber hypertrophy in satellite cell-depleted skeletal muscle
Article Snippet: .. Antibodies and reagents used were: anti-phospho-S6K1 (T389, cat# 9205), anti-phospho-S6K1 (T421/S424, cat# 9204), anti-Akt (cat# 9272), anti-phospho-Akt (T308, cat# 9275), anti-phospho-Akt (Ser473, cat# 9271), all from Cell Signaling Technology; anti-S6K1 (C-18, cat# sc-230, Santa Cruz Biotechnology); anti-Pax7, anti-embryonic myosin (F1.652) both from Developmental Studies Hybridoma Study Bank; anti-dystrophin (cat# VP D505); Mouse IgG Blocking Reagent (cat# MKB-2213); Vectashield mounting medium (cat# H-1000); streptavidin-FITC (cat# SA-5001); Alkaline Phosphatase (AP) Substrate Kit (cat# SK-5100), all from Vector Laboratories; anti-BrdU (cat# 11170376001, Roche); Texas Red-conjugated goat anti-mouse (cat# 610-109-121, Rockland Immunochemicals); biotinylated anti-mouse IgG (cat# 115-065-205, Jackson ImmunoResearch); Tyramide Signal Amplification (TSA) Kit (cat# ), DAPI (cat# D1306) both from Invitrogen; AP-conjugated goat anti-mouse IgG (cat# ab7069, Abcam). .. Gastrocnemius or plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek OCT compound (Sakura Finetek) and then quickly frozen in liquid nitrogen-cooled isopentane and stored at –80°C until sectioning.

Article Title: Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
Article Snippet: .. Antibodies and reagents used were as follows: anti-transcription factor 4 (Tcf4; cat. no. 2569), anti-phospho-SMAD3 (Ser423/425; 9520), and anti-SMAD2/3 (8685) from Cell Signaling Technology (Beverly, MA, USA); anti-Pax7, anti-myosin heavy chain I (BA.D5), anti-myosin heavy chain IIa (SC.71), and anti-myosin heavy chain IIb (BF.F3) from Developmental Studies Hybridoma Bank (Iowa City, IA, USA); anti-dystrophin (VP D505); mouse IgG blocking reagent (MKB-2213), Vectashield mounting medium (H-1000), streptavidin-FITC (SA-5001), and streptavidin-Texas Red (SA-5006) from Vector Laboratories (Burlingame, CA, USA); Texas Red-conjugated goat anti-mouse (610-109-121) from Rockland Immunochemicals (Gilbertsville, PA, USA); biotinylated anti-mouse IgG (115-065-205) from Jackson ImmunoResearch (West Grove, PA, USA); Tyramide Signal Amplification (TSA) Kit , 4′,6-diamidino-2-phenylindole (DAPI; D1306), Alexa Fluor 594 goat anti-rabbit IgG (H+L; ), wheat germ agglutinin, TX Red-X conjugate , goat anti-mouse IgG2b Alexa Fluor 647-conjugated 2°Ab (1:250; ), goat anti-mouse IgG1 Alexa Fluor 488 conjugated 2°Ab (1:500; ), and goat anti-mouse IgM biotin conjugated 2°Ab (1:150; 626840) from Invitrogen (Grand Island, NY, USA); anti-mouse pan-macrophage marker (F4/80; 14–4810-82) from eBiosciences (San Diego, CA, USA); and anti-laminin (ab14055) and goat anti-rat biotin (ab7096) from Abcam (Cambridge, MA, USA). .. Plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek optimal cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA, USA), and then quickly frozen in liquid nitrogen-cooled isopentane and stored at −80°C until sectioning.

Blocking Assay:

Article Title: Effective fiber hypertrophy in satellite cell-depleted skeletal muscle
Article Snippet: .. Antibodies and reagents used were: anti-phospho-S6K1 (T389, cat# 9205), anti-phospho-S6K1 (T421/S424, cat# 9204), anti-Akt (cat# 9272), anti-phospho-Akt (T308, cat# 9275), anti-phospho-Akt (Ser473, cat# 9271), all from Cell Signaling Technology; anti-S6K1 (C-18, cat# sc-230, Santa Cruz Biotechnology); anti-Pax7, anti-embryonic myosin (F1.652) both from Developmental Studies Hybridoma Study Bank; anti-dystrophin (cat# VP D505); Mouse IgG Blocking Reagent (cat# MKB-2213); Vectashield mounting medium (cat# H-1000); streptavidin-FITC (cat# SA-5001); Alkaline Phosphatase (AP) Substrate Kit (cat# SK-5100), all from Vector Laboratories; anti-BrdU (cat# 11170376001, Roche); Texas Red-conjugated goat anti-mouse (cat# 610-109-121, Rockland Immunochemicals); biotinylated anti-mouse IgG (cat# 115-065-205, Jackson ImmunoResearch); Tyramide Signal Amplification (TSA) Kit (cat# ), DAPI (cat# D1306) both from Invitrogen; AP-conjugated goat anti-mouse IgG (cat# ab7069, Abcam). .. Gastrocnemius or plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek OCT compound (Sakura Finetek) and then quickly frozen in liquid nitrogen-cooled isopentane and stored at –80°C until sectioning.

Article Title: Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
Article Snippet: .. Antibodies and reagents used were as follows: anti-transcription factor 4 (Tcf4; cat. no. 2569), anti-phospho-SMAD3 (Ser423/425; 9520), and anti-SMAD2/3 (8685) from Cell Signaling Technology (Beverly, MA, USA); anti-Pax7, anti-myosin heavy chain I (BA.D5), anti-myosin heavy chain IIa (SC.71), and anti-myosin heavy chain IIb (BF.F3) from Developmental Studies Hybridoma Bank (Iowa City, IA, USA); anti-dystrophin (VP D505); mouse IgG blocking reagent (MKB-2213), Vectashield mounting medium (H-1000), streptavidin-FITC (SA-5001), and streptavidin-Texas Red (SA-5006) from Vector Laboratories (Burlingame, CA, USA); Texas Red-conjugated goat anti-mouse (610-109-121) from Rockland Immunochemicals (Gilbertsville, PA, USA); biotinylated anti-mouse IgG (115-065-205) from Jackson ImmunoResearch (West Grove, PA, USA); Tyramide Signal Amplification (TSA) Kit , 4′,6-diamidino-2-phenylindole (DAPI; D1306), Alexa Fluor 594 goat anti-rabbit IgG (H+L; ), wheat germ agglutinin, TX Red-X conjugate , goat anti-mouse IgG2b Alexa Fluor 647-conjugated 2°Ab (1:250; ), goat anti-mouse IgG1 Alexa Fluor 488 conjugated 2°Ab (1:500; ), and goat anti-mouse IgM biotin conjugated 2°Ab (1:150; 626840) from Invitrogen (Grand Island, NY, USA); anti-mouse pan-macrophage marker (F4/80; 14–4810-82) from eBiosciences (San Diego, CA, USA); and anti-laminin (ab14055) and goat anti-rat biotin (ab7096) from Abcam (Cambridge, MA, USA). .. Plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek optimal cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA, USA), and then quickly frozen in liquid nitrogen-cooled isopentane and stored at −80°C until sectioning.

Article Title: Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle
Article Snippet: .. Antibodies (Abs) and reagents used were as follows: anti-dystrophin (1:50; Vector Laboratories, catalog no. VP D505), mouse IgG blocking reagent (catalog no. MKB-2213), streptavidin-AMCA (Alexa Fluor 350; catalog no. SA-5008) all from Vector Laboratories (Burlingame, CA); Texas Red-conjugated goat anti-mouse (catalog no. 610–109-121, Rockland Immunochemicals, Gilbertsville, PA); 4′,6-diamidino-2-phenylindole (DAPI; catalog no. D3571), goat anti-mouse IgG2b, Alexa Fluor 647 conjugated 2° Ab (1:250; catalog no. ), goat anti-mouse IgG1 , Alexa Fluor 488 conjugated 2° Ab (1:500; catalog no. ), goat anti-mouse IgM, biotin conjugated 2° Ab (1:150; catalog no. 626840; Invitrogen, Carlsbad, CA); and anti-MHC I (BA.D5), anti-MHC IIa (SC.71), and anti-MHC IIb (BF.F3) from Developmental Studies Hybridoma Study Bank (Iowa City, IA). ..

Article Title: Automated image analysis of skeletal muscle fiber cross-sectional area
Article Snippet: .. Antibodies and reagents used were anti-dystrophin (cat. no. VP D505); mouse IgG blocking reagent (cat. no. MKB-2213); Vectashield mounting medium (cat. no. H-1000), all from Vector Laboratories (Burlingame, CA); Texas Red-conjugated goat anti-mouse (cat. no. 610-109-121, Rockland Immunochemicals, Gilbertsville, PA); and DAPI (cat. no. D3571, Invitrogen, Carlsbad, CA). ..

IA:

Article Title: Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
Article Snippet: .. Antibodies and reagents used were as follows: anti-transcription factor 4 (Tcf4; cat. no. 2569), anti-phospho-SMAD3 (Ser423/425; 9520), and anti-SMAD2/3 (8685) from Cell Signaling Technology (Beverly, MA, USA); anti-Pax7, anti-myosin heavy chain I (BA.D5), anti-myosin heavy chain IIa (SC.71), and anti-myosin heavy chain IIb (BF.F3) from Developmental Studies Hybridoma Bank (Iowa City, IA, USA); anti-dystrophin (VP D505); mouse IgG blocking reagent (MKB-2213), Vectashield mounting medium (H-1000), streptavidin-FITC (SA-5001), and streptavidin-Texas Red (SA-5006) from Vector Laboratories (Burlingame, CA, USA); Texas Red-conjugated goat anti-mouse (610-109-121) from Rockland Immunochemicals (Gilbertsville, PA, USA); biotinylated anti-mouse IgG (115-065-205) from Jackson ImmunoResearch (West Grove, PA, USA); Tyramide Signal Amplification (TSA) Kit , 4′,6-diamidino-2-phenylindole (DAPI; D1306), Alexa Fluor 594 goat anti-rabbit IgG (H+L; ), wheat germ agglutinin, TX Red-X conjugate , goat anti-mouse IgG2b Alexa Fluor 647-conjugated 2°Ab (1:250; ), goat anti-mouse IgG1 Alexa Fluor 488 conjugated 2°Ab (1:500; ), and goat anti-mouse IgM biotin conjugated 2°Ab (1:150; 626840) from Invitrogen (Grand Island, NY, USA); anti-mouse pan-macrophage marker (F4/80; 14–4810-82) from eBiosciences (San Diego, CA, USA); and anti-laminin (ab14055) and goat anti-rat biotin (ab7096) from Abcam (Cambridge, MA, USA). .. Plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek optimal cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA, USA), and then quickly frozen in liquid nitrogen-cooled isopentane and stored at −80°C until sectioning.

Article Title: Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle
Article Snippet: .. Antibodies (Abs) and reagents used were as follows: anti-dystrophin (1:50; Vector Laboratories, catalog no. VP D505), mouse IgG blocking reagent (catalog no. MKB-2213), streptavidin-AMCA (Alexa Fluor 350; catalog no. SA-5008) all from Vector Laboratories (Burlingame, CA); Texas Red-conjugated goat anti-mouse (catalog no. 610–109-121, Rockland Immunochemicals, Gilbertsville, PA); 4′,6-diamidino-2-phenylindole (DAPI; catalog no. D3571), goat anti-mouse IgG2b, Alexa Fluor 647 conjugated 2° Ab (1:250; catalog no. ), goat anti-mouse IgG1 , Alexa Fluor 488 conjugated 2° Ab (1:500; catalog no. ), goat anti-mouse IgM, biotin conjugated 2° Ab (1:150; catalog no. 626840; Invitrogen, Carlsbad, CA); and anti-MHC I (BA.D5), anti-MHC IIa (SC.71), and anti-MHC IIb (BF.F3) from Developmental Studies Hybridoma Study Bank (Iowa City, IA). ..

Incubation:

Article Title: Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity but does not contribute to sarcopenia
Article Snippet: .. For BrdU detection, unfixed slides were incubated in an antibody against dystrophin followed by secondary antibody conjugated to Texas-Red (Cat#610-109-121, Rockland Immunochemicals Inc., Gilbertsville, PA). ..

other:

Article Title: Expression of muscle-specific ribosomal protein L3-like impairs myotube growth
Article Snippet: Hemagglutinin (HA; H6908) and fast myosin heavy chain (MyHC; H6908), (Sigma-Aldrich; St. Louis, MO, USA); goat anti-rabbit Alexa Fluor 488 (A-11070) (Life Technologies; Grand Island, NY, USA); goat anti-mouse Alexa Fluor 494 (#610-109-121) (Rockland; Gilbertsville, PA, USA); Rps6 (2317) and HA (2367) (Cell Signaling Technology; Danvers, MA, USA); puromycin (MABE343) (Millipore, Temecula, CA, USA); α-tubulin (Ab52866) (Abcam; Cambridge, MA, USA); anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Vector Laboratories; Burlingame, CA, USA).

Marker:

Article Title: Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy
Article Snippet: .. Antibodies and reagents used were as follows: anti-transcription factor 4 (Tcf4; cat. no. 2569), anti-phospho-SMAD3 (Ser423/425; 9520), and anti-SMAD2/3 (8685) from Cell Signaling Technology (Beverly, MA, USA); anti-Pax7, anti-myosin heavy chain I (BA.D5), anti-myosin heavy chain IIa (SC.71), and anti-myosin heavy chain IIb (BF.F3) from Developmental Studies Hybridoma Bank (Iowa City, IA, USA); anti-dystrophin (VP D505); mouse IgG blocking reagent (MKB-2213), Vectashield mounting medium (H-1000), streptavidin-FITC (SA-5001), and streptavidin-Texas Red (SA-5006) from Vector Laboratories (Burlingame, CA, USA); Texas Red-conjugated goat anti-mouse (610-109-121) from Rockland Immunochemicals (Gilbertsville, PA, USA); biotinylated anti-mouse IgG (115-065-205) from Jackson ImmunoResearch (West Grove, PA, USA); Tyramide Signal Amplification (TSA) Kit , 4′,6-diamidino-2-phenylindole (DAPI; D1306), Alexa Fluor 594 goat anti-rabbit IgG (H+L; ), wheat germ agglutinin, TX Red-X conjugate , goat anti-mouse IgG2b Alexa Fluor 647-conjugated 2°Ab (1:250; ), goat anti-mouse IgG1 Alexa Fluor 488 conjugated 2°Ab (1:500; ), and goat anti-mouse IgM biotin conjugated 2°Ab (1:150; 626840) from Invitrogen (Grand Island, NY, USA); anti-mouse pan-macrophage marker (F4/80; 14–4810-82) from eBiosciences (San Diego, CA, USA); and anti-laminin (ab14055) and goat anti-rat biotin (ab7096) from Abcam (Cambridge, MA, USA). .. Plantaris muscle was pinned to a cork block at resting length, covered with a thin layer of Tissue Tek optimal cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA, USA), and then quickly frozen in liquid nitrogen-cooled isopentane and stored at −80°C until sectioning.

Chloramphenicol Acetyltransferase Assay:

Article Title: Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity but does not contribute to sarcopenia
Article Snippet: .. For BrdU detection, unfixed slides were incubated in an antibody against dystrophin followed by secondary antibody conjugated to Texas-Red (Cat610-109-121, Rockland Immunochemicals Inc., Gilbertsville, PA). ..

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    Rockland Immunochemicals streptavidin conjugated alkaline phosphatase
    Streptavidin Conjugated Alkaline Phosphatase, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated alkaline phosphatase/product/Rockland Immunochemicals
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated alkaline phosphatase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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