Structured Review

Merck KGaA streptavidin agarose
Streptavidin Agarose, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin agarose/product/Merck KGaA
Average 94 stars, based on 5 article reviews
Price from $9.99 to $1999.99
streptavidin agarose - by Bioz Stars, 2020-04
94/100 stars

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Centrifugation:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: Cell debris was removed by centrifugation (7400 × g for 30 min with an R13A angle rotor and CR22N centrifuge (Hitachi)). .. The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin.

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). ..

Recombinant:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: Paragraph title: Plasmid construction and recombinant protein production ... To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Filtration:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin. .. The resulting Dcytb solution was concentrated to ~4–5 mg mL−1 by centrifugal ultrafiltration and purified by gel filtration chromatography (HiLoad 16/600 Superdex 200 pg; GE Healthcare) equilibrated with MES-Na buffer (50 mM, pH 6.5) containing NaCl (150 mM) and DDM (0.025%).

In Vitro:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: The GST-tag was removed from eluted GSTCK2α fusion protein by biotinylated thrombin protease (Merck, Germany) for in vitro kinase assay. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Chromatography:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin. .. The resulting Dcytb solution was concentrated to ~4–5 mg mL−1 by centrifugal ultrafiltration and purified by gel filtration chromatography (HiLoad 16/600 Superdex 200 pg; GE Healthcare) equilibrated with MES-Na buffer (50 mM, pH 6.5) containing NaCl (150 mM) and DDM (0.025%).

Isolation:

Article Title: Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression
Article Snippet: The Cell Surface Protein Isolation Kit, which included sulfosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate (sulfo-NHS-SS-biotin), was purchased from Pierce (Rockford, IL). .. Streptavidin agarose was purchased from Merck (Darmstadt, Germany).

Flow Cytometry:

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: The column flow through was collected and incubated with streptavidin agarose to remove thrombin from the sample. .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).

Purification:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: Paragraph title: Expression and purification of Dcytb ... The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin.

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: Briefly, purified protein was incubated with biotinylated thrombin at a final concentration of 1 unit thrombin per milligram tagged protein for 16 hours at 4°C. .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: After spin-down to remove the SOB media, cells were broken by sonication, and the tagged proteins were purified with GST-Bind agarose resin (Elpis Biotech, Korea) or His-Bind agarose resin (Elpis Biotech, Korea) according to the manufacturers’ instructions. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Concentration Assay:

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: Briefly, purified protein was incubated with biotinylated thrombin at a final concentration of 1 unit thrombin per milligram tagged protein for 16 hours at 4°C. .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).

Incubation:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: .. The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin. ..

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: The column flow through was collected and incubated with streptavidin agarose to remove thrombin from the sample. .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature. .. The eluates were concentrated using Amicon Ultra (Millipore, Germany).

Molecular Weight:

Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
Article Snippet: .. Streptavidin agarose was removed from the sample by centrifugation and the protein was concentrated using Amico Ultra-15 concentrators, 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). ..

Expressing:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: Paragraph title: Expression and purification of Dcytb ... The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin.

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: For expression in E. coli , the fragments were inserted into pGEX-4T-1 for glutathione S-transferase (GST)-tagged Hd6/CK2α protein (GST-CK2α) and the modified pET28a for His(6x)-Maltose binding protein (HisMBP)- tagged PRR37 protein (HisMBP-PRR37) and HisMBP-LHYc [C-terminal region (520–719 residues) of OsLHY; Ogiso et al., 2010]. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Lysis:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: Nonspecifically bound material was eluted from this column with lysis buffer containing 10 mM imidazole (pH 5.8) (washing buffer), and the solubilized Dcytb was eluted by the same buffer containing 500 mM imidazole (pH 5.8). .. The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin.

Modification:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: For expression in E. coli , the fragments were inserted into pGEX-4T-1 for glutathione S-transferase (GST)-tagged Hd6/CK2α protein (GST-CK2α) and the modified pET28a for His(6x)-Maltose binding protein (HisMBP)- tagged PRR37 protein (HisMBP-PRR37) and HisMBP-LHYc [C-terminal region (520–719 residues) of OsLHY; Ogiso et al., 2010]. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Sonication:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: After spin-down to remove the SOB media, cells were broken by sonication, and the tagged proteins were purified with GST-Bind agarose resin (Elpis Biotech, Korea) or His-Bind agarose resin (Elpis Biotech, Korea) according to the manufacturers’ instructions. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Crystallization Assay:

Article Title: Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate
Article Snippet: The hexa-His tag was cleaved by overnight treatment with biotinylated thrombin (Merck Millipore) at 20 °C followed by incubation of this reaction mixture with streptavidin agarose (Merck Millipore) for 1 h at 4 °C to capture the biotinylated thrombin. .. The peak fractions were collected and concentrated (~15 mg mL−1 ) for crystallization with a centrifugal ultrafiltration unit (Amicon, Merck Millipore) having a 50 kDa cutoff.

Binding Assay:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: For expression in E. coli , the fragments were inserted into pGEX-4T-1 for glutathione S-transferase (GST)-tagged Hd6/CK2α protein (GST-CK2α) and the modified pET28a for His(6x)-Maltose binding protein (HisMBP)- tagged PRR37 protein (HisMBP-PRR37) and HisMBP-LHYc [C-terminal region (520–719 residues) of OsLHY; Ogiso et al., 2010]. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Kinase Assay:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: The GST-tag was removed from eluted GSTCK2α fusion protein by biotinylated thrombin protease (Merck, Germany) for in vitro kinase assay. .. To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

Plasmid Preparation:

Article Title: Casein Kinases I and 2α Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice
Article Snippet: Paragraph title: Plasmid construction and recombinant protein production ... To remove the thrombin, GST-CK2α eluate was incubated with streptavidin agarose (Merck, Germany) for 30 min at room temperature.

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    Merck KGaA streptavidin agarose
    Streptavidin Agarose, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose/product/Merck KGaA
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    Merck KGaA bovine serum albumin bsa
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    https://www.bioz.com/result/bovine serum albumin bsa/product/Merck KGaA
    Average 99 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin bsa - by Bioz Stars, 2020-04
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