streptavidin agarose solution  (Millipore)


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    Structured Review

    Millipore streptavidin agarose solution
    P cr -400 interacts with the coiled-coil of MBP-Fishhook. (A) MBP (lanes 2 and 3), MBP-Hairpin (lanes 4 and 5), or MBP-Fishhook (lanes 6 and 7) was incubated without (−) or with (+) P cr -400, and the complexes were analyzed by native PAGE and stained with Coomasie blue. P cr -400 induces a shift in the electrophoretic mobility of the trimeric MBP-Fishhook (T F ), causing it to migrate similarly to trimeric MBP-Hairpin (T H ). A, aggregate. Lane M, molecular-mass markers. (B) Increasing amounts of P cr -400 were incubated with MBP-Fishhook, and the complexes were analyzed by native PAGE. Lanes 2 and 3, MBP-Hairpin and MBP-Fishhook controls. The amount of MBP-Fishhook (T F ) shifting to a complex that mimics MBP-Hairpin (T H ) increases with the amount of P cr -400 added (lanes 4 to 12). (C) Increasing amounts of biotinylated P cr -400 were incubated with MBP-Fishhook (lanes 4 to 8). As a control, MBP was also incubated with biotinylated P cr -400 (lane 9). <t>Streptavidin-agarose</t> was then added to the reaction mixture, and the bound complexes were precipitated, heat denatured, and analyzed by SDS-PAGE.
    Streptavidin Agarose Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose solution/product/Millipore
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose solution - by Bioz Stars, 2020-04
    86/100 stars

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    Images

    1) Product Images from "An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein"

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein

    Journal: Journal of Virology

    doi: 10.1128/JVI.77.5.3281-3290.2003

    P cr -400 interacts with the coiled-coil of MBP-Fishhook. (A) MBP (lanes 2 and 3), MBP-Hairpin (lanes 4 and 5), or MBP-Fishhook (lanes 6 and 7) was incubated without (−) or with (+) P cr -400, and the complexes were analyzed by native PAGE and stained with Coomasie blue. P cr -400 induces a shift in the electrophoretic mobility of the trimeric MBP-Fishhook (T F ), causing it to migrate similarly to trimeric MBP-Hairpin (T H ). A, aggregate. Lane M, molecular-mass markers. (B) Increasing amounts of P cr -400 were incubated with MBP-Fishhook, and the complexes were analyzed by native PAGE. Lanes 2 and 3, MBP-Hairpin and MBP-Fishhook controls. The amount of MBP-Fishhook (T F ) shifting to a complex that mimics MBP-Hairpin (T H ) increases with the amount of P cr -400 added (lanes 4 to 12). (C) Increasing amounts of biotinylated P cr -400 were incubated with MBP-Fishhook (lanes 4 to 8). As a control, MBP was also incubated with biotinylated P cr -400 (lane 9). Streptavidin-agarose was then added to the reaction mixture, and the bound complexes were precipitated, heat denatured, and analyzed by SDS-PAGE.
    Figure Legend Snippet: P cr -400 interacts with the coiled-coil of MBP-Fishhook. (A) MBP (lanes 2 and 3), MBP-Hairpin (lanes 4 and 5), or MBP-Fishhook (lanes 6 and 7) was incubated without (−) or with (+) P cr -400, and the complexes were analyzed by native PAGE and stained with Coomasie blue. P cr -400 induces a shift in the electrophoretic mobility of the trimeric MBP-Fishhook (T F ), causing it to migrate similarly to trimeric MBP-Hairpin (T H ). A, aggregate. Lane M, molecular-mass markers. (B) Increasing amounts of P cr -400 were incubated with MBP-Fishhook, and the complexes were analyzed by native PAGE. Lanes 2 and 3, MBP-Hairpin and MBP-Fishhook controls. The amount of MBP-Fishhook (T F ) shifting to a complex that mimics MBP-Hairpin (T H ) increases with the amount of P cr -400 added (lanes 4 to 12). (C) Increasing amounts of biotinylated P cr -400 were incubated with MBP-Fishhook (lanes 4 to 8). As a control, MBP was also incubated with biotinylated P cr -400 (lane 9). Streptavidin-agarose was then added to the reaction mixture, and the bound complexes were precipitated, heat denatured, and analyzed by SDS-PAGE.

    Techniques Used: Incubation, Clear Native PAGE, Staining, SDS Page

    2) Product Images from "Mechanism of antigen presentation after hypertonic loading of soluble antigens"

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens

    Journal: Immunology

    doi: 10.1046/j.1365-2567.2002.01476.x

    Isolation of biotinylated OVA from EL-4 cells immediately after loading (lanes 2 and 5) and after 2 hr (lanes 3 and 6) and 4 hr (lanes 4 and 7). The OVA was precipitated from the cell lysate using Streptavidin–agarose and separated by 12% PAGE under reducing conditions. After blotting, nitrocellulose was assayed with polyclonal antibody to OVA (lanes 1–3) or proteasomes (lanes 4–6). Lane 1, size markers stained with India ink (67 000, 43 000, 30 000 and 20 000 MW).
    Figure Legend Snippet: Isolation of biotinylated OVA from EL-4 cells immediately after loading (lanes 2 and 5) and after 2 hr (lanes 3 and 6) and 4 hr (lanes 4 and 7). The OVA was precipitated from the cell lysate using Streptavidin–agarose and separated by 12% PAGE under reducing conditions. After blotting, nitrocellulose was assayed with polyclonal antibody to OVA (lanes 1–3) or proteasomes (lanes 4–6). Lane 1, size markers stained with India ink (67 000, 43 000, 30 000 and 20 000 MW).

    Techniques Used: Isolation, Polyacrylamide Gel Electrophoresis, Staining

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein
    Article Snippet: Nine hundred microliters of RIPA buffer (PBS-0.25% Triton X-100) and 50 μl of streptavidin-agarose solution (Sigma) were added, and the reaction mixture was incubated at 4°C for 1 h with gentle rotation. .. The proteins were heated in sample buffer and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE).

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr. .. The pellet was resuspended in SDS–polyacrylamide gel electrophoresis (PAGE) sample buffer, boiled for 2 min and analysed on a 12% polyacrylamide gel under reducing conditions.

    Centrifugation:

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein
    Article Snippet: Nine hundred microliters of RIPA buffer (PBS-0.25% Triton X-100) and 50 μl of streptavidin-agarose solution (Sigma) were added, and the reaction mixture was incubated at 4°C for 1 h with gentle rotation. .. The bound complexes were pelleted by centrifugation and washed five times with RIPA buffer.

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: .. The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr. ..

    Protease Inhibitor:

    Article Title: Lutein transport by Caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI)
    Article Snippet: N -Acetyl-β- D -glucopyranoside (manufactured by Calbiochem) and streptavidin–agarose solution (manufactured by Oncogene Research Products) were obtained from VWR International SAS (Strasbourg, France). .. Protease inhibitor cocktail was a gift from F. Tosini (Avantage Nutrition, Marseille, France).

    Cell Culture:

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: After extensive washing, the cells were either lysed immediately or cultured at 37° in a 5% CO2 incubator. .. The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr.

    Polymerase Chain Reaction:

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein
    Article Snippet: Approximately 1.0 mg of Pcr -400 peptide was biotinylated using EZ-link PEO-Iodoacetyl biotin (Pierce) as directed by the manufacturer. .. Nine hundred microliters of RIPA buffer (PBS-0.25% Triton X-100) and 50 μl of streptavidin-agarose solution (Sigma) were added, and the reaction mixture was incubated at 4°C for 1 h with gentle rotation.

    Incubation:

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein
    Article Snippet: .. Nine hundred microliters of RIPA buffer (PBS-0.25% Triton X-100) and 50 μl of streptavidin-agarose solution (Sigma) were added, and the reaction mixture was incubated at 4°C for 1 h with gentle rotation. .. The bound complexes were pelleted by centrifugation and washed five times with RIPA buffer.

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: .. The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein
    Article Snippet: Nine hundred microliters of RIPA buffer (PBS-0.25% Triton X-100) and 50 μl of streptavidin-agarose solution (Sigma) were added, and the reaction mixture was incubated at 4°C for 1 h with gentle rotation. .. The proteins were heated in sample buffer and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE).

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr. .. The pellet was resuspended in SDS–polyacrylamide gel electrophoresis (PAGE) sample buffer, boiled for 2 min and analysed on a 12% polyacrylamide gel under reducing conditions.

    Modification:

    Article Title: Lutein transport by Caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI)
    Article Snippet: N -Acetyl-β- D -glucopyranoside (manufactured by Calbiochem) and streptavidin–agarose solution (manufactured by Oncogene Research Products) were obtained from VWR International SAS (Strasbourg, France). .. DMEM (Dulbecco's modified Eagle's medium) containing 4.5 g/l glucose and trypsin/EDTA (500 and 200 mg/l respectively) were purchased from BioWhittaker (Fontenay-sous-Bois, France), FBS (foetal bovine serum) was from Biomedia (Issy-les-Moulineaux, France), and non-essential amino acids, penicillin/streptomycin, PBS and PBS containing 0.1 mM CaCl2 and 1 mM MgCl2 (PBSCM) were from Gibco BRL (Cergy-Pontoise, France).

    Lysis:

    Article Title: Mechanism of antigen presentation after hypertonic loading of soluble antigens
    Article Snippet: For lysis, cells were resuspended in a hypotonic solution [Tris–EDTA–dithiothreitol (DTT), 50–1–1 m m , containing azide and 0·2 m m PMSF] and homogenized on ice with a Dounce type homogenizer. .. The cell lysate was cleared by centrifugation at 15 000 g for 10 min. To the lysate, 50 µl of a preabsorbed 4% Streptavidin–agarose solution (Sigma) was added and the mixture was incubated at 4° under constant rotation for 2 hr.

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  • News
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  • 86
    Millipore streptavidin agarose solution
    Streptavidin Agarose Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose solution/product/Millipore
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose solution - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    85
    Millipore streptavidine agarose solution
    Streptavidine Agarose Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidine agarose solution/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidine agarose solution - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

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