streptavidin agarose dynabeads  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Millipore streptavidin agarose dynabeads
    Streptavidin Agarose Dynabeads, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose dynabeads/product/Millipore
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose dynabeads - by Bioz Stars, 2020-04
    86/100 stars

    Images

    Related Articles

    Filtration:

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: The unincorporated label was removed by gel filtration on a Sephadex G25 column ( ). .. Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma).

    Ethanol Precipitation:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was purified by phenol/chloroform extraction and ethanol precipitation, and then annealed with a 5-OHU-containing 5′-phosphorylated 32-nucleotide oligo (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was extracted with phenol/chloroform followed by ethanol precipitation and then annealed with 5-OHU-containing 5′-phosphorylated 32-nt oligonucleotide (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.

    Generated:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: Similar repair reactions were carried with a circular duplex DNA substrate containing a single 5-OHU residue that was generated as described previously ( , ). .. Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma).

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: The repair substrate pUC19CPD plasmid containing a single 5-OHU was generated as described previously ( , ). .. Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma).

    Labeling:

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: To produce 5′-32 P-labeled substrates, the ss oligonucleotides were labeled at the 5′ terminus with [γ-32 P]ATP and T4 PNK before annealing. .. Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma).

    Purification:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: Paragraph title: BER Assay with Purified Proteins ... Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma).

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma). .. 5-OHU-containing form I plasmid generated by sealing the nick with T4 DNA ligase was purified by equilibrium ultracentrifugation in CsCl/ethidium bromide.

    Incubation:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: A linear 51-bp duplex containing a 5-OHU base lesion at position 26 in one strand (2 pmol) was incubated with NEIL1, PNKP, Polβ, (50 fmol of each), and DNA ligase IIIα-XRCC1 (25 or 50 fmol) for 30 min at 37 °C in BER buffer containing 1 mmol of ATP, 25 mmol of dATP, dTTP, and dGTP, 5 μmol of dCTP, and 10 μmol of [α-32 P]dCTP. .. Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma).

    Binding Assay:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: .. Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was purified by phenol/chloroform extraction and ethanol precipitation, and then annealed with a 5-OHU-containing 5′-phosphorylated 32-nucleotide oligo (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: .. Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was extracted with phenol/chloroform followed by ethanol precipitation and then annealed with 5-OHU-containing 5′-phosphorylated 32-nt oligonucleotide (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.

    Plasmid Preparation:

    Article Title: The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites *
    Article Snippet: .. Briefly, the plasmid pUC19CPD containing two single-strand nicking sites on the same strand, 32 nucleotides apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-mer oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′) that was removed by annealing with an of excess biotinylated complementary 32-nucleotide oligonucleotide followed by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was purified by phenol/chloroform extraction and ethanol precipitation, and then annealed with a 5-OHU-containing 5′-phosphorylated 32-nucleotide oligo (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.

    Article Title: Specific Inhibition of NEIL-initiated Repair of Oxidized Base Damage in Human Genome by Copper and Iron
    Article Snippet: .. Briefly, the parent plasmid containing two ss nicking sites on the same strand, 32 nt apart, was digested with N.BstNB1 (New England Biolabs) and heated at 65 °C for 10 min to dissociate the 32-nt ss oligonucleotide (5′-GCG GAT ATT AAT GTG ACG GTA GCG AGT CGC TC-3′), which was then annealed with excess biotinylated complementary 32-nt oligonucleotide and removed from the solution by binding to streptavidin-agarose Dynabeads (Sigma). .. The gapped plasmid was extracted with phenol/chloroform followed by ethanol precipitation and then annealed with 5-OHU-containing 5′-phosphorylated 32-nt oligonucleotide (5′-pGCG GAT ATT AAT GTG ACG G 5-OHU A GCG AGT CGC TC-3′) at 45 °C in TE buffer containing 50 m m NaCl.