streptavidin agarose beads slurry  (Millipore)


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    Structured Review

    Millipore streptavidin agarose beads slurry
    Streptavidin Agarose Beads Slurry, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose beads slurry/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose beads slurry - by Bioz Stars, 2020-04
    80/100 stars

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    Centrifugation:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: The nuclei were pelleted by centrifugation at 7,500 ×g for 20–30 s, resuspended in 100 μ L of buffer C (20 mM HEPES (pH 7.9), 1.5 mM MgCl2 , 0.2 mM EDTA, 420 mM NaCl, and 25% (v/v) glycerol, with 1 × protease inhibitor cocktail), and incubated on ice for 20 min. .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    Affinity Precipitation:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Paragraph title: 2.6. Nuclear Extract Preparation and DNA Affinity Precipitation Assay (DAPA) ... Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    Synthesized:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Biotinylated oligonucleotides covering the LXRα SREBP-1c LXRE oligonucleotides (for HepaRG cells, 5′-biotin-CAG TGA CCG CCA GTA ACC CCA GC-3′) or ABCG1 LXRE oligonucleotides (for LS174T cells, 5′-biotin- GGC AAG AGG TAA CTG TCG GTC AAA TCC T-3′) were synthesized and used as probes. .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    Protease Inhibitor:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: The nuclei were pelleted by centrifugation at 7,500 ×g for 20–30 s, resuspended in 100 μ L of buffer C (20 mM HEPES (pH 7.9), 1.5 mM MgCl2 , 0.2 mM EDTA, 420 mM NaCl, and 25% (v/v) glycerol, with 1 × protease inhibitor cocktail), and incubated on ice for 20 min. .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    Sampling:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA). .. Bound protein complexes were eluted using 1 × SDS-PAGE sampling buffer and then analyzed by Western blotting analysis with specific antibodies against SRC-1 and SMILE.

    Incubation:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA). ..

    CTG Assay:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Biotinylated oligonucleotides covering the LXRα SREBP-1c LXRE oligonucleotides (for HepaRG cells, 5′-biotin-CAG TGA CCG CCA GTA ACC CCA GC-3′) or ABCG1 LXRE oligonucleotides (for LS174T cells, 5′-biotin- GGC AAG AGG TAA CTG TCG GTC AAA TCC T-3′) were synthesized and used as probes. .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    Western Blot:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA). .. Bound protein complexes were eluted using 1 × SDS-PAGE sampling buffer and then analyzed by Western blotting analysis with specific antibodies against SRC-1 and SMILE.

    Binding Assay:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: We used DAPA to identify the interaction between transcription factors and coregulators and to assess binding to the specific promoter response elements and the effects of drug treatments on binding. .. Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA).

    SDS Page:

    Article Title: Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)
    Article Snippet: Nuclear extracts (500 μ g) from treated HepaRG and LS174T cells were exposed to T090 (10 μ M) alone or in combination with 20 or 40 μ M SSM for 24 h and incubated with these biotinylated probes (2 μ g each) and 20 μ L of 50% (w/v) streptavidin-agarose beads slurry (Sigma-Aldrich, St. Louis, MO, USA). .. Bound protein complexes were eluted using 1 × SDS-PAGE sampling buffer and then analyzed by Western blotting analysis with specific antibodies against SRC-1 and SMILE.

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  • 90
    Millipore streptavidin agarose bead slurry
    Streptavidin Agarose Bead Slurry, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose bead slurry/product/Millipore
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose bead slurry - by Bioz Stars, 2020-04
    90/100 stars
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