streptavadin hrp  (Vector Laboratories)


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    Structured Review

    Vector Laboratories streptavadin hrp
    Streptavadin Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavadin hrp/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavadin hrp - by Bioz Stars, 2021-09
    86/100 stars

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    Related Articles

    Activity Assay:

    Article Title: A mammalian acetate switch regulates stress erythropoiesis
    Article Snippet: .. We quenched endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide (Cat. No. H1009-100ML, Sigma) in deionized water for 30 min and rinsing in deionized water twice for 5 min. For Acss2 antibody (Cat. No. 3658, Cell Signaling Technology) staining, we incubated slides with the BD Retrievagen A working solution (Cat. No. 550524, BD Biosciences, San Jose, CA) and heated to ∼90 °C (193 ° F) in a microwave oven for 10 min, followed by slow cooling to room temperature over 60 min. We then rinsed slides twice with deionized water, and next rinsed once with PBS for 10 min. We blocked non-specific binding by incubation with 10% goat serum (Cat. No. S-1000, Vector Laboratories, Burlingame, CA) in PBS for 60 min. Next, we incubated slides with Acss2 primary antibody (1:150 in 10% goat serum with PBS) overnight at 4 °C and then with biotin-conjugated goat anti-rabbit IgG (1:500 in 10% goat serum with PBS) for 2 h. After rinsing in PBS, we incubated slides with HRP conjugated streptavadin (1:750 in 10% goat serum with PBS) for 60 min at room temperature. ..

    Staining:

    Article Title: A mammalian acetate switch regulates stress erythropoiesis
    Article Snippet: .. We quenched endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide (Cat. No. H1009-100ML, Sigma) in deionized water for 30 min and rinsing in deionized water twice for 5 min. For Acss2 antibody (Cat. No. 3658, Cell Signaling Technology) staining, we incubated slides with the BD Retrievagen A working solution (Cat. No. 550524, BD Biosciences, San Jose, CA) and heated to ∼90 °C (193 ° F) in a microwave oven for 10 min, followed by slow cooling to room temperature over 60 min. We then rinsed slides twice with deionized water, and next rinsed once with PBS for 10 min. We blocked non-specific binding by incubation with 10% goat serum (Cat. No. S-1000, Vector Laboratories, Burlingame, CA) in PBS for 60 min. Next, we incubated slides with Acss2 primary antibody (1:150 in 10% goat serum with PBS) overnight at 4 °C and then with biotin-conjugated goat anti-rabbit IgG (1:500 in 10% goat serum with PBS) for 2 h. After rinsing in PBS, we incubated slides with HRP conjugated streptavadin (1:750 in 10% goat serum with PBS) for 60 min at room temperature. ..

    Incubation:

    Article Title: A mammalian acetate switch regulates stress erythropoiesis
    Article Snippet: .. We quenched endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide (Cat. No. H1009-100ML, Sigma) in deionized water for 30 min and rinsing in deionized water twice for 5 min. For Acss2 antibody (Cat. No. 3658, Cell Signaling Technology) staining, we incubated slides with the BD Retrievagen A working solution (Cat. No. 550524, BD Biosciences, San Jose, CA) and heated to ∼90 °C (193 ° F) in a microwave oven for 10 min, followed by slow cooling to room temperature over 60 min. We then rinsed slides twice with deionized water, and next rinsed once with PBS for 10 min. We blocked non-specific binding by incubation with 10% goat serum (Cat. No. S-1000, Vector Laboratories, Burlingame, CA) in PBS for 60 min. Next, we incubated slides with Acss2 primary antibody (1:150 in 10% goat serum with PBS) overnight at 4 °C and then with biotin-conjugated goat anti-rabbit IgG (1:500 in 10% goat serum with PBS) for 2 h. After rinsing in PBS, we incubated slides with HRP conjugated streptavadin (1:750 in 10% goat serum with PBS) for 60 min at room temperature. ..

    Article Title: The Bisecting GlcNAc on N-Glycans Inhibits Growth Factor Signaling and Retards Mammary Tumor Progression
    Article Snippet: .. For lectin blotting, membranes were blocked in 5% non-fat milk, incubated with biotinylated-E-PHA or - leukophytohemagglutinin (L-PHA; Vector Labs) at 5 μg/ml at room temperature for 1 h, washed with TBS-Tween, incubated with streptavadin-HRP (1:5000;Vector Labs) for 1h, and visualized using an ECL kit. ..

    Article Title: AN IMPORTANT ROLE FOR GRANULOCYTES IN THE THERMAL REGULATION OF COLON TUMOR GROWTH
    Article Snippet: .. This was followed by washing in PBS three times, incubation with streptavadin, three more washes, and then DAB peroxidase substrate (Vector Laboratories, Inc., Bulingame, CA) was applied. ..

    Article Title: Increased survivin expression contributes to apoptosis-resistance in IPF fibroblasts
    Article Snippet: .. Sections were washed twice in TBS and treated with a 1 in 6 solution of goat serum containing 4 drops/ml of Avidin block (Vector Laboratories, Peterborough, UK) for 20 minutes and incubated overnight with rabbit anti-survivin polyclonal antibodies (4 µg/ml; Santa Cruz Biotechnology) or an isotype control in TBS with 1% bovine serum albumin and 4 drops/ml of Biotin block. ..

    Binding Assay:

    Article Title: A mammalian acetate switch regulates stress erythropoiesis
    Article Snippet: .. We quenched endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide (Cat. No. H1009-100ML, Sigma) in deionized water for 30 min and rinsing in deionized water twice for 5 min. For Acss2 antibody (Cat. No. 3658, Cell Signaling Technology) staining, we incubated slides with the BD Retrievagen A working solution (Cat. No. 550524, BD Biosciences, San Jose, CA) and heated to ∼90 °C (193 ° F) in a microwave oven for 10 min, followed by slow cooling to room temperature over 60 min. We then rinsed slides twice with deionized water, and next rinsed once with PBS for 10 min. We blocked non-specific binding by incubation with 10% goat serum (Cat. No. S-1000, Vector Laboratories, Burlingame, CA) in PBS for 60 min. Next, we incubated slides with Acss2 primary antibody (1:150 in 10% goat serum with PBS) overnight at 4 °C and then with biotin-conjugated goat anti-rabbit IgG (1:500 in 10% goat serum with PBS) for 2 h. After rinsing in PBS, we incubated slides with HRP conjugated streptavadin (1:750 in 10% goat serum with PBS) for 60 min at room temperature. ..

    Avidin-Biotin Assay:

    Article Title: Increased survivin expression contributes to apoptosis-resistance in IPF fibroblasts
    Article Snippet: .. Sections were washed twice in TBS and treated with a 1 in 6 solution of goat serum containing 4 drops/ml of Avidin block (Vector Laboratories, Peterborough, UK) for 20 minutes and incubated overnight with rabbit anti-survivin polyclonal antibodies (4 µg/ml; Santa Cruz Biotechnology) or an isotype control in TBS with 1% bovine serum albumin and 4 drops/ml of Biotin block. ..

    Blocking Assay:

    Article Title: Increased survivin expression contributes to apoptosis-resistance in IPF fibroblasts
    Article Snippet: .. Sections were washed twice in TBS and treated with a 1 in 6 solution of goat serum containing 4 drops/ml of Avidin block (Vector Laboratories, Peterborough, UK) for 20 minutes and incubated overnight with rabbit anti-survivin polyclonal antibodies (4 µg/ml; Santa Cruz Biotechnology) or an isotype control in TBS with 1% bovine serum albumin and 4 drops/ml of Biotin block. ..

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  • 86
    Vector Laboratories streptavadin hrp
    Streptavadin Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavadin hrp/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavadin hrp - by Bioz Stars, 2021-09
    86/100 stars
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