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Stratagene strataclone solo pack competent cells
Strataclone Solo Pack Competent Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/strataclone solo pack competent cells/product/Stratagene
Average 83 stars, based on 1 article reviews
Price from $9.99 to $1999.99
strataclone solo pack competent cells - by Bioz Stars, 2020-03
83/100 stars

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Clone Assay:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: .. PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was purified using the Wizard Plus SV Miniprep Kit (Promega) and sequenced on an ABI 3130 Genetic Analyzer sequencer using the BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Synthesized:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: Total RNA was extracted from stage 42 X. laevis embryos using the RNeasy Maxi Kit (Qiagen), and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s instructions. .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: Total RNA was extracted from stage 40 X. laevis embryos using the RNeasy Maxi kit (Qiagen), and cDNA was synthesized using the iScript cDNA Synthesis kit (Bio-Rad) following manufacturers’ instructions. .. PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene).

Isolation:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Purification:

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was purified using the Wizard Plus SV Miniprep Kit (Promega) and sequenced on an ABI 3130 Genetic Analyzer sequencer using the BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).

Polymerase Chain Reaction:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: .. PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was purified using the Wizard Plus SV Miniprep Kit (Promega) and sequenced on an ABI 3130 Genetic Analyzer sequencer using the BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).

Sequencing:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: Paragraph title: Cloning and Sequence Analysis ... RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: Paragraph title: Cloning and Sequence Analysis ... PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene).

Transformation Assay:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: .. PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was purified using the Wizard Plus SV Miniprep Kit (Promega) and sequenced on an ABI 3130 Genetic Analyzer sequencer using the BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).

Plasmid Preparation:

Article Title: Cloning and characterization of GABAA ? subunits and GABAB subunits in Xenopus laevis during development
Article Snippet: .. RT-PCR was performed using Supertaq DNA Polymerase kit (Ambion), with annealing temperatures between 55°C and 64°C and annealing times between 1.5 and 2.5 minutes, and PCR products were cloned into the Strataclone pSC-A-amp/kan vector and transformed using Strataclone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was isolated using the Wizard Plus SV Miniprep Kit (Promega) and the Plasmid Midiprep Kit (Bio-Rad).

Article Title: Cloning and Characterization of Voltage-Gated Calcium Channel Alpha1 Subunits in Xenopus laevis During Development
Article Snippet: .. PCR products were cloned into the pCRII-TOPO vector and transformed into One Shot DH5α Chemically Competent Escherichia coli (Invitrogen) or into the Strata-Clone pSC-A-amp/kan vector and transformed using StrataClone Solo-Pack Competent Cells (Stratagene). .. Plasmid DNA containing the cloned PCR products was purified using the Wizard Plus SV Miniprep Kit (Promega) and sequenced on an ABI 3130 Genetic Analyzer sequencer using the BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).

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    Stratagene strataclone solo pack competent cells
    Strataclone Solo Pack Competent Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strataclone solo pack competent cells/product/Stratagene
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strataclone solo pack competent cells - by Bioz Stars, 2020-03
    83/100 stars
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