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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the <t>E68</t> strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
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Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the E68 strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Journal: Synthetic and Systems Biotechnology

Article Title: Metabolic engineering of Saccharomyces cerevisiae for co-production of ergothioneine, salidroside and gadusol

doi: 10.1016/j.synbio.2026.04.010

Figure Lengend Snippet: Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the E68 strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Article Snippet: Transformants were randomly selected from selective plates for fermentation validation, yielding strain E68, which achieved an EGT titer of 50 mg L −1 ( B).

Techniques: