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The incidence of IBD outbreaks in Khartoum State 2003–2004 and vaccination program.

Journal: Open Veterinary Journal

Article Title: Epidemiology and laboratory diagnosis of very virulent infectious bursal disease virus in vaccinated chickens in Khartoum, Sudan

doi: 10.5455/OVJ.2022.v12.i1.5

Figure Lengend Snippet: The incidence of IBD outbreaks in Khartoum State 2003–2004 and vaccination program.

Article Snippet: One freeze-dried ampule of vaccine strain D78 (Intervet International, Boxmeer, Holland) was dissolved in 1 ml sterile double distilled water (DDW), and 200 μl was taken for RNA extraction by TRIzol method as control positive as described before.

Techniques:

Ethidium bromide-stained Agarose gel (1%). RT-PCR was carried out on RNA samples extracted from bursas of infected birds using primers IBDV-F/IBDV-R. Lane 1 control negative (DDW), lane 2 control negative (bursa from uninfected bird), lane 3 control positive (D78 IBD vaccine), lanes 4, 5 and 6 representative bursal samples from the field cases of IBD in Sudan, Lane M 100 bp marker.

Journal: Open Veterinary Journal

Article Title: Epidemiology and laboratory diagnosis of very virulent infectious bursal disease virus in vaccinated chickens in Khartoum, Sudan

doi: 10.5455/OVJ.2022.v12.i1.5

Figure Lengend Snippet: Ethidium bromide-stained Agarose gel (1%). RT-PCR was carried out on RNA samples extracted from bursas of infected birds using primers IBDV-F/IBDV-R. Lane 1 control negative (DDW), lane 2 control negative (bursa from uninfected bird), lane 3 control positive (D78 IBD vaccine), lanes 4, 5 and 6 representative bursal samples from the field cases of IBD in Sudan, Lane M 100 bp marker.

Article Snippet: One freeze-dried ampule of vaccine strain D78 (Intervet International, Boxmeer, Holland) was dissolved in 1 ml sterile double distilled water (DDW), and 200 μl was taken for RNA extraction by TRIzol method as control positive as described before.

Techniques: Staining, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Infection, Control, Marker

Virucidal and bactericidal efficacies of GSE against viruses and bacteria in aqueous phase

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibitory effect of grapefruit seed extract (GSE) on avian pathogens

doi: 10.1292/jvms.18-0754

Figure Lengend Snippet: Virucidal and bactericidal efficacies of GSE against viruses and bacteria in aqueous phase

Article Snippet: Low pathogenic AIV, A/duck/Akita/714/06 (H5N2) [ ], NDV strain Sato [ ] and IBDV vaccine strain D78 (Intervet Co., Ltd., Tokyo, Japan) were used to evaluate the in vitro virucidal effect of GSE.

Techniques: Bacteria, Positive Control