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jingzhaotoxin iii  (Alomone Labs)


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    Alomone Labs jingzhaotoxin iii
    Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and <t>JZTX-III</t> (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.
    Jingzhaotoxin Iii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stj-200/pmc05557004-63-43-45?v=Alomone+Labs
    Average 90 stars, based on 2 article reviews
    jingzhaotoxin iii - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca 2+ Transients in Cultured Skeletal Muscle Fibers"

    Article Title: Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca 2+ Transients in Cultured Skeletal Muscle Fibers

    Journal: Journal of Diabetes Research

    doi: 10.1155/2017/1509048

    Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and JZTX-III (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.
    Figure Legend Snippet: Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and JZTX-III (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.

    Techniques Used: Inhibition, Cell Culture



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    jingzhaotoxin iii  (Alomone Labs)
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    Alomone Labs jingzhaotoxin iii
    Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and <t>JZTX-III</t> (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.
    Jingzhaotoxin Iii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stj-200/pmc05557004-63-43-45?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    jingzhaotoxin iii - by Bioz Stars, 2026-07
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    jingzhaotoxin iii jztx iii  (Alomone Labs)
    90
    Alomone Labs jingzhaotoxin iii jztx iii
    A TTX-insensitive current is present in immature calyces. (A) Control I Na and currents remaining in the presence of 200 nM TTX at membrane potentials above –60 mV in a P8 calyx (note different current scales for left and right panels). Voltage protocol similar to Figure : a 40 ms step to –130 mV from a holding potential of –80 mV was followed by a series of 40 ms depolarizing steps in 10 mV increments from –90 to +20 mV. (B) Control (black) and response to 200 nM TTX (red) for nine calyces (P5–11) are shown in the IV plot of peak inward currents for steps between –80 and 0 mV. (C) Left panel: the residual current following 200 nM TTX is blocked in 1 μM TTX and the block reverses with washout in a P6 calyx. Currents in response to a voltage step from –130 to –30 mV. Right panel: 1 <t>μM</t> <t>JZTX-III</t> blocks a component of I Na and the remaining current is abolished following application of 1 μM JZTX-III plus 200 nM TTX in a P7 calyx. Currents in response to a voltage step from –130 to –50 mV. (D) Summary for a group of 12 cells perfused with 200 nM TTX (six cells at P5–6, six at P8–11). A group of cells exposed to 1 μM TTX ( n = 8, one cell at P6, seven cells at P7–10) I Na was abolished in 1 μM JZTX-III and 200 nM TTX ( n = 4, two cells at P6 and two cells at P7). Peak inward current was measured at –50 mV step. I Na tended to be larger at younger ages as shown by distributions. In the presence of 200 nM TTX, I Na decreased from –3.9 (1.8) to –0.25 (0.5) nA ( P < 0.001, paired t-test, n = 12). Remaining I Na ranged from 0.008 to 1.8 nA. In all cells exposed to 1 μM TTX, I Na was completely abolished ( n = 8, P = 0.001). I Na was also completely abolished in a group of cells perfused with a combination of 1 μM JZTX-III and 200 nM TTX ( n = 4, P = 0.022). External solution was L-15 in the TTX experiments, and 80 mM Na + HEPES in the JZTX-III/200 nM TTX experiments; electrode solution Cs + . ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.
    Jingzhaotoxin Iii Jztx Iii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stj-200/pmc06246661-82-3-7?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    jingzhaotoxin iii jztx iii - by Bioz Stars, 2026-07
    90/100 stars
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    Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and JZTX-III (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.

    Journal: Journal of Diabetes Research

    Article Title: Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca 2+ Transients in Cultured Skeletal Muscle Fibers

    doi: 10.1155/2017/1509048

    Figure Lengend Snippet: Inhibition of ion channels known to modulate the excitable properties of skeletal muscle: impact on biphasic AP-transients on 5-day old cultured fibers challenged with elevated glucose. Representative time course of a biphasic AP-induced Ca 2+ transient (left panels) measured in fibers challenged with elevated D-glucose (25 mM; 48 h.) before (black traces) and 10 minutes after (red traces) the treatment with gadolinium (100 μ M) (a), apamin (1 μ M) (b), and JZTX-III (1 μ M) (c). Panels (d–f) are zoomed-in versions of the records shown in (a–c) to better appreciate biphasic responses before and after channel blockers addition. No significant changes in time course of the Ca 2+ transient (i.e., interspike interval) were found in fibers challenged with 25 mM D-glucose-exposed fibers and treated with gadolinium ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), apamin ( n = 8 fibers; p > 0.05, two-sample paired Student's t -test), or JZTX-III ( n = 6 fibers; p > 0.05, two-sample paired Student's t -test), when compared to fibers challenged with D-glucose.

    Article Snippet: To assess the contribution of different ion channels to the development of the biphasic action potential Ca 2+ transient, 5-day-old cultured fibers were exposed to either gadolinium (Axxora, San Diego, CA, catalog number 400-023-M500), apamin (Sigma-Aldrich, St. Louis, MO, catalog number A-1289), or Jingzhaotoxin-III (JZTX-III; Alomone Labs, Jerusalem, IL, catalog number STJ-200), blockers of mechanosensitivity, SK channels, and Na v 1.5 channels, respectively.

    Techniques: Inhibition, Cell Culture

    A TTX-insensitive current is present in immature calyces. (A) Control I Na and currents remaining in the presence of 200 nM TTX at membrane potentials above –60 mV in a P8 calyx (note different current scales for left and right panels). Voltage protocol similar to Figure : a 40 ms step to –130 mV from a holding potential of –80 mV was followed by a series of 40 ms depolarizing steps in 10 mV increments from –90 to +20 mV. (B) Control (black) and response to 200 nM TTX (red) for nine calyces (P5–11) are shown in the IV plot of peak inward currents for steps between –80 and 0 mV. (C) Left panel: the residual current following 200 nM TTX is blocked in 1 μM TTX and the block reverses with washout in a P6 calyx. Currents in response to a voltage step from –130 to –30 mV. Right panel: 1 μM JZTX-III blocks a component of I Na and the remaining current is abolished following application of 1 μM JZTX-III plus 200 nM TTX in a P7 calyx. Currents in response to a voltage step from –130 to –50 mV. (D) Summary for a group of 12 cells perfused with 200 nM TTX (six cells at P5–6, six at P8–11). A group of cells exposed to 1 μM TTX ( n = 8, one cell at P6, seven cells at P7–10) I Na was abolished in 1 μM JZTX-III and 200 nM TTX ( n = 4, two cells at P6 and two cells at P7). Peak inward current was measured at –50 mV step. I Na tended to be larger at younger ages as shown by distributions. In the presence of 200 nM TTX, I Na decreased from –3.9 (1.8) to –0.25 (0.5) nA ( P < 0.001, paired t-test, n = 12). Remaining I Na ranged from 0.008 to 1.8 nA. In all cells exposed to 1 μM TTX, I Na was completely abolished ( n = 8, P = 0.001). I Na was also completely abolished in a group of cells perfused with a combination of 1 μM JZTX-III and 200 nM TTX ( n = 4, P = 0.022). External solution was L-15 in the TTX experiments, and 80 mM Na + HEPES in the JZTX-III/200 nM TTX experiments; electrode solution Cs + . ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Regional and Developmental Differences in Na + Currents in Vestibular Primary Afferent Neurons

    doi: 10.3389/fncel.2018.00423

    Figure Lengend Snippet: A TTX-insensitive current is present in immature calyces. (A) Control I Na and currents remaining in the presence of 200 nM TTX at membrane potentials above –60 mV in a P8 calyx (note different current scales for left and right panels). Voltage protocol similar to Figure : a 40 ms step to –130 mV from a holding potential of –80 mV was followed by a series of 40 ms depolarizing steps in 10 mV increments from –90 to +20 mV. (B) Control (black) and response to 200 nM TTX (red) for nine calyces (P5–11) are shown in the IV plot of peak inward currents for steps between –80 and 0 mV. (C) Left panel: the residual current following 200 nM TTX is blocked in 1 μM TTX and the block reverses with washout in a P6 calyx. Currents in response to a voltage step from –130 to –30 mV. Right panel: 1 μM JZTX-III blocks a component of I Na and the remaining current is abolished following application of 1 μM JZTX-III plus 200 nM TTX in a P7 calyx. Currents in response to a voltage step from –130 to –50 mV. (D) Summary for a group of 12 cells perfused with 200 nM TTX (six cells at P5–6, six at P8–11). A group of cells exposed to 1 μM TTX ( n = 8, one cell at P6, seven cells at P7–10) I Na was abolished in 1 μM JZTX-III and 200 nM TTX ( n = 4, two cells at P6 and two cells at P7). Peak inward current was measured at –50 mV step. I Na tended to be larger at younger ages as shown by distributions. In the presence of 200 nM TTX, I Na decreased from –3.9 (1.8) to –0.25 (0.5) nA ( P < 0.001, paired t-test, n = 12). Remaining I Na ranged from 0.008 to 1.8 nA. In all cells exposed to 1 μM TTX, I Na was completely abolished ( n = 8, P = 0.001). I Na was also completely abolished in a group of cells perfused with a combination of 1 μM JZTX-III and 200 nM TTX ( n = 4, P = 0.022). External solution was L-15 in the TTX experiments, and 80 mM Na + HEPES in the JZTX-III/200 nM TTX experiments; electrode solution Cs + . ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

    Article Snippet: Stock solutions of Jingzhaotoxin-III (JZTX-III) and TTX (Alomone Labs, Jerusalem, Israel) dissolved in deionized water were stored at −20°C until the day of use.

    Techniques: Blocking Assay