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Atlanta Biologicals sterile hepes buffered rpmi 1640 culture medium
Sterile Hepes Buffered Rpmi 1640 Culture Medium, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sterile hepes buffered rpmi 1640 culture medium/product/Atlanta Biologicals
Average 81 stars, based on 4 article reviews
Price from $9.99 to $1999.99
sterile hepes buffered rpmi 1640 culture medium - by Bioz Stars, 2020-04
81/100 stars

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Fluorescence:

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml. .. Purified splenic or genital cells contained at least 90% CD3+ cells, as determined by fluorescence-activated cell sorter analysis.

Article Title: Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial Infection
Article Snippet: Explants were transferred to 7 ml of a 0.6-mg/ml concentration of filter-sterilized type I collagenase (Atlanta Biologicals). .. Purified genital tract cells contained at least 97% CD3+ cells, as determined by fluorescence-activated cell sorter analysis.

Article Title: Route of Infection That Induces a High Intensity of Gamma Interferon-Secreting T Cells in the Genital Tract Produces Optimal Protection against Chlamydia trachomatis Infection in Mice
Article Snippet: Explants were transferred to 7 ml of filter-sterilized type I collagenase (0.6 mg/ml; Atlanta Biologicals). .. Purified genital tract cells contained at least 97% CD3+ cells, as determined by fluorescence-activated cell sorting analysis.

Infection:

Article Title: The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice
Article Snippet: .. The level of Th1 response and recruitment into the genital mucosa was determined by measuring the response of chlamydial‐specific, IFN‐γ‐secreting T cells in the genital tract tissues of infected mice, as previously described Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method , as follows: at the indicated time after infection, animals in each group were killed and the genital tract between the vagina and ovaries (i.e. the cervix, uterus and Fallopian tubes) was excised and placed in sterile HEPES‐buffered RPMI‐1640 culture medium (Atlanta Biologicals, Norcross, GA). .. Explants were transferred to 7 ml of 0·6 mg/ml filter‐sterilized type I collagenase (Atlanta Biologicals).

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: Briefly, immune T-cell-enriched cells were prepared from the genital tract tissues of immunized and infected mice by the collagenase digestion and nylon wool enrichment method ( , ) as follows: at the indicated time after immunization or infection, animals in each group were sacrificed, and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised and placed in sterile HEPES ( N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid)-buffered RPMI 1640 culture medium (Atlanta Biologicals, Norcross, Ga.). .. Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml.

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: .. Briefly, immune T-cell-enriched cells were prepared from the genital tract tissues of immunized and infected mice by the collagenase digestion and nylon wool enrichment method ( , ) as follows: at the indicated time after immunization or infection, animals in each group were sacrificed, and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised and placed in sterile HEPES ( N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid)-buffered RPMI 1640 culture medium (Atlanta Biologicals, Norcross, Ga.). .. Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml.

Article Title: Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial Infection
Article Snippet: At the indicated time after infection, the animals in each group were sacrificed, and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised from each mouse and placed in sterile HEPES ( N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid)-buffered RPMI 1640 medium (Atlanta Biologicals, Norcross, Ga.). .. Explants were transferred to 7 ml of a 0.6-mg/ml concentration of filter-sterilized type I collagenase (Atlanta Biologicals).

Article Title: Route of Infection That Induces a High Intensity of Gamma Interferon-Secreting T Cells in the Genital Tract Produces Optimal Protection against Chlamydia trachomatis Infection in Mice
Article Snippet: Paragraph title: Preparation of T cells from the genital tracts of infected mice and assessment of amount of IFN-γ secreted into culture supernatants. ... Explants were transferred to 7 ml of filter-sterilized type I collagenase (0.6 mg/ml; Atlanta Biologicals).

Purification:

Article Title: The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice
Article Snippet: The level of Th1 response and recruitment into the genital mucosa was determined by measuring the response of chlamydial‐specific, IFN‐γ‐secreting T cells in the genital tract tissues of infected mice, as previously described Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method , as follows: at the indicated time after infection, animals in each group were killed and the genital tract between the vagina and ovaries (i.e. the cervix, uterus and Fallopian tubes) was excised and placed in sterile HEPES‐buffered RPMI‐1640 culture medium (Atlanta Biologicals, Norcross, GA). .. Following washing, the cells were enriched for T cells by the nylon wool adherence method., Purified genital tract cells contained at least 97% CD3+ cells, as determined by FACS analysis.

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml. .. Purified splenic or genital cells contained at least 90% CD3+ cells, as determined by fluorescence-activated cell sorter analysis.

Article Title: Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial Infection
Article Snippet: Explants were transferred to 7 ml of a 0.6-mg/ml concentration of filter-sterilized type I collagenase (Atlanta Biologicals). .. Purified genital tract cells contained at least 97% CD3+ cells, as determined by fluorescence-activated cell sorter analysis.

Article Title: Route of Infection That Induces a High Intensity of Gamma Interferon-Secreting T Cells in the Genital Tract Produces Optimal Protection against Chlamydia trachomatis Infection in Mice
Article Snippet: Explants were transferred to 7 ml of filter-sterilized type I collagenase (0.6 mg/ml; Atlanta Biologicals). .. Purified genital tract cells contained at least 97% CD3+ cells, as determined by fluorescence-activated cell sorting analysis.

Concentration Assay:

Article Title: Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial Infection
Article Snippet: .. Explants were transferred to 7 ml of a 0.6-mg/ml concentration of filter-sterilized type I collagenase (Atlanta Biologicals). ..

Incubation:

Article Title: The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice
Article Snippet: The level of Th1 response and recruitment into the genital mucosa was determined by measuring the response of chlamydial‐specific, IFN‐γ‐secreting T cells in the genital tract tissues of infected mice, as previously described Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method , as follows: at the indicated time after infection, animals in each group were killed and the genital tract between the vagina and ovaries (i.e. the cervix, uterus and Fallopian tubes) was excised and placed in sterile HEPES‐buffered RPMI‐1640 culture medium (Atlanta Biologicals, Norcross, GA). .. The tissues were minced, incubated at 37° for 45–60 min, then teased with forceps, and passed through a cell strainer.

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml. .. The tissues were minced, incubated at 37°C for 45 to 60 min, then teased with forceps, and passed through a cell strainer.

FACS:

Article Title: The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice
Article Snippet: The level of Th1 response and recruitment into the genital mucosa was determined by measuring the response of chlamydial‐specific, IFN‐γ‐secreting T cells in the genital tract tissues of infected mice, as previously described Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method , as follows: at the indicated time after infection, animals in each group were killed and the genital tract between the vagina and ovaries (i.e. the cervix, uterus and Fallopian tubes) was excised and placed in sterile HEPES‐buffered RPMI‐1640 culture medium (Atlanta Biologicals, Norcross, GA). .. Following washing, the cells were enriched for T cells by the nylon wool adherence method., Purified genital tract cells contained at least 97% CD3+ cells, as determined by FACS analysis.

Article Title: Route of Infection That Induces a High Intensity of Gamma Interferon-Secreting T Cells in the Genital Tract Produces Optimal Protection against Chlamydia trachomatis Infection in Mice
Article Snippet: Explants were transferred to 7 ml of filter-sterilized type I collagenase (0.6 mg/ml; Atlanta Biologicals). .. Purified genital tract cells contained at least 97% CD3+ cells, as determined by fluorescence-activated cell sorting analysis.

Mouse Assay:

Article Title: The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice
Article Snippet: .. The level of Th1 response and recruitment into the genital mucosa was determined by measuring the response of chlamydial‐specific, IFN‐γ‐secreting T cells in the genital tract tissues of infected mice, as previously described Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method , as follows: at the indicated time after infection, animals in each group were killed and the genital tract between the vagina and ovaries (i.e. the cervix, uterus and Fallopian tubes) was excised and placed in sterile HEPES‐buffered RPMI‐1640 culture medium (Atlanta Biologicals, Norcross, GA). .. Explants were transferred to 7 ml of 0·6 mg/ml filter‐sterilized type I collagenase (Atlanta Biologicals).

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: Briefly, immune T-cell-enriched cells were prepared from the genital tract tissues of immunized and infected mice by the collagenase digestion and nylon wool enrichment method ( , ) as follows: at the indicated time after immunization or infection, animals in each group were sacrificed, and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised and placed in sterile HEPES ( N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid)-buffered RPMI 1640 culture medium (Atlanta Biologicals, Norcross, Ga.). .. Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml.

Article Title: Route of Infection That Induces a High Intensity of Gamma Interferon-Secreting T Cells in the Genital Tract Produces Optimal Protection against Chlamydia trachomatis Infection in Mice
Article Snippet: Paragraph title: Preparation of T cells from the genital tracts of infected mice and assessment of amount of IFN-γ secreted into culture supernatants. ... Briefly, at the indicated time after infection, animals in each group were sacrificed and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised and placed in sterile HEPES-buffered RPMI 1640 culture medium (Atlanta Biologicals, Norcross, Ga.).

Article Title: Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein-Lipophilic Immune Response-Stimulating Complexes
Article Snippet: .. Briefly, immune T-cell-enriched cells were prepared from the genital tract tissues of immunized and infected mice by the collagenase digestion and nylon wool enrichment method ( , ) as follows: at the indicated time after immunization or infection, animals in each group were sacrificed, and the genital tract between the vagina and ovaries (i.e., the cervix, uterus, and fallopian tubes) was excised and placed in sterile HEPES ( N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid)-buffered RPMI 1640 culture medium (Atlanta Biologicals, Norcross, Ga.). .. Explants were transferred to 7 ml of 0.6 mg of filter-sterilized type I collagenase (Atlanta Biologicals) per ml.

Article Title: Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial Infection
Article Snippet: Briefly, immune T cells were prepared from the genital tract tissues of infected mice by the collagenase digestion method ( , ) as follows. .. Explants were transferred to 7 ml of a 0.6-mg/ml concentration of filter-sterilized type I collagenase (Atlanta Biologicals).

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    Atlanta Biologicals sterile hepes n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid buffered rpmi 1640 culture medium
    Sterile Hepes N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid Buffered Rpmi 1640 Culture Medium, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile hepes n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid buffered rpmi 1640 culture medium/product/Atlanta Biologicals
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sterile hepes n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid buffered rpmi 1640 culture medium - by Bioz Stars, 2020-04
    85/100 stars
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