steponeplus thermocycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher steponeplus thermocycler
    Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus thermocycler/product/Thermo Fisher
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    steponeplus thermocycler - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Before performing q-RT-PCR analysis, a final-point PCR was carried out for all genes to test the primers and verify the amplified products. .. Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions.

    Article Title: Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
    Article Snippet: .. The resulting cDNA was amplified by real time PCR with a StepOnePlus thermocycler (Applied Biosystems, CA) using the PowerUp SYBR Green master mix (Thermo, MA) and 100 nM of each primer. .. The primers, annealing temperatures and amplification efficiencies are listed in Supplementary Table .

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA. .. Prior to analysis within sorted luminal and basal populations from the adult virgin mammary gland, RNA was amplified by the BCM Genomic and RNA Profiling Core using the NuGen WT-Ovation Kit (NuGen, San Carlos, CA).

    Article Title: Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis
    Article Snippet: The amplification efficiencies of all primer pairs were between 95 and 102%. .. Real-time PCR was done in a StepOnePlus Thermocycler (Applied Biosystems, Foster City, CA) using a program with an initial denaturation step at 95°C for 30 seconds followed by 40 cycles of 95°C for 3 seconds and 60°C for 30 seconds.

    Article Title: Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts
    Article Snippet: A primer was deemed non-specific and discarded from further consideration if more than 10% of reactions with non-target DNA resulted in cycle thresholds (CT ) of 35 or lower or if post-cycling melt curve analysis indicated the presence of multiple amplification products. .. Real-time PCR and melt curve analyses were performed with Power SYBR Green MasterMix chemistry on a StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA). .. Ct value indicates the number of cycles that were required to detect the initiation of amplification of the cDNA amplicon.

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference. .. The cycling conditions used were as follows: amplification stage – 95 °C for 10 min and then 40 amplification cycles of 95 °C for 30 s, 60 °C for 1 min and 72 °C for 30 s; melting curve stage – 95 °C for 15 s, 60 °C for 1 min and 95 °C for 15 s. No-template and no-reverse transcriptase reactions served as the negative controls.

    Synthesized:

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: Total RNA was isolated using RNeasy micro kit (Qiagen). cDNA was synthesized using qScript cDNA Supermix (Quanta Biosciences). .. Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Local TNFR1 signaling licenses murine neutrophils for increased TLR-dependent cytokine and eicosanoid production
    Article Snippet: Neutrophils were lysed in RNAzol (MRC) and RNA was purified according to manufacturer’s instructions and concentrated using on a column (RNA Clean and Concentrator, Zymo Research). cDNA was prepared with iScript cDNA synthesis kit (Biorad), and quantitative PCR was performed with SYBR Green Master mix (Biorad) on a StepOnePlus thermocycler (Applied Biosystems). .. Primers were obtained from PrimerBank ( ) and synthesized by IDT (see ).

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: After the cDNA was synthesized, real-time PCR was performed using iTaq™ SYBR® Green Supermix with ROX (BioRad, Hercules, CA) to detect the transcript levels of Notch receptors and Notch target genes. .. A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA).

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: RNA was extracted as described above. cDNA was synthesized using the iScript reverse transcriptase kit (Bio-Rad). .. The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference.

    Quantitative RT-PCR:

    Article Title: Local TNFR1 signaling licenses murine neutrophils for increased TLR-dependent cytokine and eicosanoid production
    Article Snippet: Paragraph title: Quantitative RT-PCR ... Neutrophils were lysed in RNAzol (MRC) and RNA was purified according to manufacturer’s instructions and concentrated using on a column (RNA Clean and Concentrator, Zymo Research). cDNA was prepared with iScript cDNA synthesis kit (Biorad), and quantitative PCR was performed with SYBR Green Master mix (Biorad) on a StepOnePlus thermocycler (Applied Biosystems).

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: Paragraph title: Quantitative RT-PCR (qPCR) ... The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference.

    Real-time Polymerase Chain Reaction:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Paragraph title: Quantitative real-time PCR analysis of gene expression ... Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions.

    Article Title: Modeling appendicular skeletal cartilage development with modified high-density micromass cultures of adult human bone marrow-derived mesenchymal progenitor cells
    Article Snippet: .. Quantitative real-time PCR was performed using a StepOnePlus thermocycler (Applied Biosystems) and SYBR Green Reaction Mix (Applied Biosystems). .. Expression of aggrecan (ACAN ) and collagen type II (COL2A1 ) was quantified to analyze chondrogenic differentiation.

    Article Title: Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
    Article Snippet: .. The resulting cDNA was amplified by real time PCR with a StepOnePlus thermocycler (Applied Biosystems, CA) using the PowerUp SYBR Green master mix (Thermo, MA) and 100 nM of each primer. .. The primers, annealing temperatures and amplification efficiencies are listed in Supplementary Table .

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA. ..

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: .. Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems). .. The thermal cycling conditions comprised of an initial denaturing step at 95°C for 20 s, and 40 cycles at 95°C for 3 s, 60°C for 30 s. The PCR cycle threshold (Ct) values were measured within the exponential phase of the PCR reaction using StepOnePlus software version 2.1 (Applied Biosystems).

    Article Title: Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis
    Article Snippet: .. Real-time PCR was done in a StepOnePlus Thermocycler (Applied Biosystems, Foster City, CA) using a program with an initial denaturation step at 95°C for 30 seconds followed by 40 cycles of 95°C for 3 seconds and 60°C for 30 seconds. ..

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: TaqMan qPCR reactions contained 5 μl of eluted DNA, 300 nM forward and reverse primers against GFP, 150 nM probe, 20 μg salmon sperm DNA, and TaqMan Fast Advanced Master Mix (ThermoFisher Scientific). .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts
    Article Snippet: .. Real-time PCR and melt curve analyses were performed with Power SYBR Green MasterMix chemistry on a StepOnePlus thermocycler (Applied Biosystems). .. Following an initial 10-minute denaturation and Taq activation at 95°C, reactions were cycled as follows, using a “stepdown” scheme to encourage reaction stringency: 2 cycles of 15s at 95°C and 60s at 60°C, 2 cycles of 15s at 95°C and 60s at 59°C, and 40 cycles of 15s at 95°C and 60s at 58°C.

    Article Title: Local TNFR1 signaling licenses murine neutrophils for increased TLR-dependent cytokine and eicosanoid production
    Article Snippet: .. Neutrophils were lysed in RNAzol (MRC) and RNA was purified according to manufacturer’s instructions and concentrated using on a column (RNA Clean and Concentrator, Zymo Research). cDNA was prepared with iScript cDNA synthesis kit (Biorad), and quantitative PCR was performed with SYBR Green Master mix (Biorad) on a StepOnePlus thermocycler (Applied Biosystems). .. Primers were obtained from PrimerBank ( ) and synthesized by IDT (see ).

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: Paragraph title: Real-time PCR ... A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA).

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: .. The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference. ..

    Article Title: Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein
    Article Snippet: .. The reaction was performed with Evagreen qPCR mastermix-Rox (ABM, Richmond, BC, Canada), following the manufacturer's instructions in a StepOnePlus thermocycler (Applied Biosystems). ..

    Expressing:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Paragraph title: Quantitative real-time PCR analysis of gene expression ... Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions.

    Article Title: Modeling appendicular skeletal cartilage development with modified high-density micromass cultures of adult human bone marrow-derived mesenchymal progenitor cells
    Article Snippet: Paragraph title: Gene expression analysis ... Quantitative real-time PCR was performed using a StepOnePlus thermocycler (Applied Biosystems) and SYBR Green Reaction Mix (Applied Biosystems).

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: Paragraph title: Expression profiling ... Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems).

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: .. The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference. ..

    Transplantation Assay:

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: Cells for transplantation were never cultured on plastic to prevent loss of MaSCs and subsequent differentiation. .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA.

    Activation Assay:

    Article Title: Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts
    Article Snippet: Real-time PCR and melt curve analyses were performed with Power SYBR Green MasterMix chemistry on a StepOnePlus thermocycler (Applied Biosystems). .. Following an initial 10-minute denaturation and Taq activation at 95°C, reactions were cycled as follows, using a “stepdown” scheme to encourage reaction stringency: 2 cycles of 15s at 95°C and 60s at 60°C, 2 cycles of 15s at 95°C and 60s at 59°C, and 40 cycles of 15s at 95°C and 60s at 58°C.

    Cell Culture:

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: Cells for transplantation were never cultured on plastic to prevent loss of MaSCs and subsequent differentiation. .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA.

    Generated:

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA. ..

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: Standard curves generated from GFP cDNA plasmids were created for each assay. .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems).

    Sequencing:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Sequence homology searches were performed using the Basic Local Alignment Search Tool (Blast) “ http://www.ncbi.nlm.nih.gov/blast ”, to confirm gene identity of amplified fragments and that these primers matched only the sequence to which they were designed. .. Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions.

    Article Title: Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein
    Article Snippet: The primers for each sequence were 59-CTTGGGCACCGAAGAAACAC-39 (nucleotides 3438 to 3457) and 59-TTGGTCACGTTGCCATTCAC-39 (nucleotides 3770 to 3789) for the E2 gene and 59-AAGGGCGTAACCGAAATCGGT-39 (nucleotides 26 to 46) and 59-CATATACCTCACGTCGCAG-39 (nucleotides 215 to 233) for the E6 gene. .. The reaction was performed with Evagreen qPCR mastermix-Rox (ABM, Richmond, BC, Canada), following the manufacturer's instructions in a StepOnePlus thermocycler (Applied Biosystems).

    Fluorescence:

    Article Title: Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts
    Article Snippet: Real-time PCR and melt curve analyses were performed with Power SYBR Green MasterMix chemistry on a StepOnePlus thermocycler (Applied Biosystems). .. Fluorescence levels of the SYBR Green dye relative to ROX passive reference dye were assessed in real time at each 60-second annealing step during thermal cycling and then again with every 0.3°C increase during the melt curve analysis.

    Isolation:

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: Paragraph title: RNA Isolation and Quantitative Reverse Transcription-PCR ... RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA.

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: Total RNA was isolated using RNeasy micro kit (Qiagen). cDNA was synthesized using qScript cDNA Supermix (Quanta Biosciences). .. Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems).

    Size-exclusion Chromatography:

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems). .. As described , VSV-G pseudotyped viral particles were generated by three-plasmid transfection of 293T with Fugene-6 (Promega), using 1 μg Gag-Pol expression plasmid, 1 μg VSV-G expression plasmid pMD2.G (GenScript) or CMV-Ampho, and 1.5 μg genome vector encoding cDNAs or shRNAs.

    Purification:

    Article Title: Modeling appendicular skeletal cartilage development with modified high-density micromass cultures of adult human bone marrow-derived mesenchymal progenitor cells
    Article Snippet: Gene expression analysis Total RNA was extracted using Trizol (Invitrogen) and purified with the RNeasy Plus mini kit (Qiagen). cDNA was reverse transcribed using the SuperScript IV kit (Invitrogen). .. Quantitative real-time PCR was performed using a StepOnePlus thermocycler (Applied Biosystems) and SYBR Green Reaction Mix (Applied Biosystems).

    Article Title: Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
    Article Snippet: Purified RNA (500 ng) was used for retrotranscription with the iScript RT Supermix (Biorad, CA). .. The resulting cDNA was amplified by real time PCR with a StepOnePlus thermocycler (Applied Biosystems, CA) using the PowerUp SYBR Green master mix (Thermo, MA) and 100 nM of each primer.

    Article Title: Local TNFR1 signaling licenses murine neutrophils for increased TLR-dependent cytokine and eicosanoid production
    Article Snippet: .. Neutrophils were lysed in RNAzol (MRC) and RNA was purified according to manufacturer’s instructions and concentrated using on a column (RNA Clean and Concentrator, Zymo Research). cDNA was prepared with iScript cDNA synthesis kit (Biorad), and quantitative PCR was performed with SYBR Green Master mix (Biorad) on a StepOnePlus thermocycler (Applied Biosystems). .. Primers were obtained from PrimerBank ( ) and synthesized by IDT (see ).

    Polymerase Chain Reaction:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: PCR products were sequenced and homology searches were performed with Blast. .. Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions.

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems). .. The thermal cycling conditions comprised of an initial denaturing step at 95°C for 20 s, and 40 cycles at 95°C for 3 s, 60°C for 30 s. The PCR cycle threshold (Ct) values were measured within the exponential phase of the PCR reaction using StepOnePlus software version 2.1 (Applied Biosystems).

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: The plate was then sealed to prevent evaporation and quantitative Real Time PCR was performed using following parameters: the initial denaturation was for 10 minutes at 95°C; PCR cycling for 40 cycles was carried out for 10 seconds at 95°C and 45 seconds at 60°C. .. A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA).

    Article Title: Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein
    Article Snippet: For detection of integration, a multiplex PCR for the HPV-16 E2 and HPV-16 E6 genes was performed. .. The reaction was performed with Evagreen qPCR mastermix-Rox (ABM, Richmond, BC, Canada), following the manufacturer's instructions in a StepOnePlus thermocycler (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: Heat-treated viral supernatants (95°C, 5 min) served as controls for plasmid contamination. .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems).

    Software:

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA. ..

    Article Title: Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
    Article Snippet: Real-time PCR was performed using Fast SYBR Mastermix (Applied Biosystems) on a StepOnePlus thermocycler (Applied Biosystems). .. The thermal cycling conditions comprised of an initial denaturing step at 95°C for 20 s, and 40 cycles at 95°C for 3 s, 60°C for 30 s. The PCR cycle threshold (Ct) values were measured within the exponential phase of the PCR reaction using StepOnePlus software version 2.1 (Applied Biosystems).

    SYBR Green Assay:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions. .. The 12.5 µl PCR reaction mixtures contained 6.25 µl of 2×Power SYBR Green PCR Master Mix (Applied Biosystems), 160 nM of each primer, and 2 µl of diluted cDNA as template.

    Article Title: Modeling appendicular skeletal cartilage development with modified high-density micromass cultures of adult human bone marrow-derived mesenchymal progenitor cells
    Article Snippet: .. Quantitative real-time PCR was performed using a StepOnePlus thermocycler (Applied Biosystems) and SYBR Green Reaction Mix (Applied Biosystems). .. Expression of aggrecan (ACAN ) and collagen type II (COL2A1 ) was quantified to analyze chondrogenic differentiation.

    Article Title: Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
    Article Snippet: .. The resulting cDNA was amplified by real time PCR with a StepOnePlus thermocycler (Applied Biosystems, CA) using the PowerUp SYBR Green master mix (Thermo, MA) and 100 nM of each primer. .. The primers, annealing temperatures and amplification efficiencies are listed in Supplementary Table .

    Article Title: Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function
    Article Snippet: .. RNA was collected through extraction with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and cDNA templates were generated using a SuperScript III kit and 1 μ g RNA per sample according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA). qPCR reactions were run using SYBR Green reagent (Applied Biosystems, Carlsbad, CA) on a StepOnePlus thermocycler (Applied Biosystems, Life Technologies, Carlsbad, CA) and fold changes were calculated using the Comparative CT (ΔΔCT) method and Step One software v2.0.1, Applied Biosystems, Life Technologies, Carlsbad, CA. ..

    Article Title: Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis
    Article Snippet: Three technical replicates were done per sample in a total reaction volume of 10 μL, which contained 5 μL of PerfeCTa® SYBR® Green FastMix® (Quanta BioSciences, Inc., Gaithersburg, MD), 2.5 μL of a forward/reverse primer mix with 1.6 μM of each primer, and 2.5 μL of cDNA diluted 1:15. .. Real-time PCR was done in a StepOnePlus Thermocycler (Applied Biosystems, Foster City, CA) using a program with an initial denaturation step at 95°C for 30 seconds followed by 40 cycles of 95°C for 3 seconds and 60°C for 30 seconds.

    Article Title: Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts
    Article Snippet: .. Real-time PCR and melt curve analyses were performed with Power SYBR Green MasterMix chemistry on a StepOnePlus thermocycler (Applied Biosystems). .. Following an initial 10-minute denaturation and Taq activation at 95°C, reactions were cycled as follows, using a “stepdown” scheme to encourage reaction stringency: 2 cycles of 15s at 95°C and 60s at 60°C, 2 cycles of 15s at 95°C and 60s at 59°C, and 40 cycles of 15s at 95°C and 60s at 58°C.

    Article Title: Local TNFR1 signaling licenses murine neutrophils for increased TLR-dependent cytokine and eicosanoid production
    Article Snippet: .. Neutrophils were lysed in RNAzol (MRC) and RNA was purified according to manufacturer’s instructions and concentrated using on a column (RNA Clean and Concentrator, Zymo Research). cDNA was prepared with iScript cDNA synthesis kit (Biorad), and quantitative PCR was performed with SYBR Green Master mix (Biorad) on a StepOnePlus thermocycler (Applied Biosystems). .. Primers were obtained from PrimerBank ( ) and synthesized by IDT (see ).

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: In a 96 well plate, 2.5 μL of prepared cDNA was added to 22.5 μL of a mastermix-containing 2X SYBR Green Universal Master Mix, and 50 μM forward and reverse primers for the intended target. .. A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA).

    Article Title: The apolipoprotein N-acyl transferase Lnt is dispensable for growth in Acinetobacter species
    Article Snippet: .. The primers used for expression analysis are listed in Table S2. qPCR was carried out using POWER SYBR Green Supermix on a StepOnePlus thermocycler (Applied Biosystems), with the 16S rRNA gene as the reference. ..

    Multiplex Assay:

    Article Title: Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein
    Article Snippet: For detection of integration, a multiplex PCR for the HPV-16 E2 and HPV-16 E6 genes was performed. .. The reaction was performed with Evagreen qPCR mastermix-Rox (ABM, Richmond, BC, Canada), following the manufacturer's instructions in a StepOnePlus thermocycler (Applied Biosystems).

    Agarose Gel Electrophoresis:

    Article Title: L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy
    Article Snippet: Gene relative quantification was carried out using MicroAmp Optical 96-well plates (Applied Biosystems) in a StepOnePlus thermocycler (Applied Biosystems) in standard cycling conditions. .. The primer specificity and the formation of primer-dimers were confirmed by melt curve analysis and agarose gel electrophoresis.

    Spectrophotometry:

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: Concentration of each DNA sample was quantified using a NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific). .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems).

    Evaporation:

    Article Title: Inhibition of HER2 Increases Jagged1-dependent Breast Cancer Stem Cells: Role for Membrane Jagged1
    Article Snippet: The plate was then sealed to prevent evaporation and quantitative Real Time PCR was performed using following parameters: the initial denaturation was for 10 minutes at 95°C; PCR cycling for 40 cycles was carried out for 10 seconds at 95°C and 45 seconds at 60°C. .. A melt curve was added after completion of the 40 cycles set by the StepOnePlus thermocycler manufacturer (Applied Biosystems, Foster City, CA).

    Concentration Assay:

    Article Title: Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling
    Article Snippet: Concentration of each DNA sample was quantified using a NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific). .. Cycle parameters for gene detection were 95°C for 20 sec, then 40 cycles of 95°C for 1 sec, 60°C for 20 sec, using a StepOnePlus thermocycler (Applied Biosystems).

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  • 99
    Thermo Fisher steponeplus real time pcr system thermocycler
    Steponeplus Real Time Pcr System Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus real time pcr system thermocycler/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    steponeplus real time pcr system thermocycler - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher abi steponeplus thermocycler
    Abi Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus thermocycler/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus thermocycler - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher automatic thermocycler
    Automatic Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/automatic thermocycler/product/Thermo Fisher
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    automatic thermocycler - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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