steponeplus real time pcr machine  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    StepOnePlus Real Time PCR System
    Description:
    The StepOnePlus Real Time PCR System is a 96 well Real Time PCR instrument perfect for both first time and experienced users The StepOnePlus Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 4 color optical recording the StepOnePlus Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications We supply a Dell Laptop with the StepOnePlus Real Time PCR System Features of the StepOnePlus Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays• Sensitive 4 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageNew StepOne SoftwareThe StepOne Software included with the StepOnePlus Real Time PCR System runs on the Windows XP operating system and provides instrument control data collection and data analysis capabilities analysis software is also compatible with Windows 7 This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOnePlus Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 4 Color Fluorescence ReadingThe StepOnePlus Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE NED TAMRA and ROX dyes This cost effective 4 color 96 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus Real Time PCR System and StepOne Software The StepOnePlus System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Protein Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis as a standalone application • High Resolution Melting as a standalone application Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOnePlus System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOnePlus Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOnePlus Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Energy Efficiency and Space SavingThe StepOnePlus Real Time PCR System draws 38 less energy to process one sample plate when the instrument is in a heated state compared to the Bio Rad CFX96 Real Time PCR Detection System The StepOnePlus Real Time PCR System also has advanced temperature control through use of VeriFlex Blocks technology and has a footprint nearly 15 smaller than Bio Rad CFX96 which in today s crowded laboratories helps you use your laboratory space even more efficiently You also leave a smaller environmental footprint on the Earth Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOnePlus Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionAdvanced High Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOnePlus Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate
    Catalog Number:
    4376598
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Gene Expression Analysis & Genotyping|Genotyping Instruments, Software & Calibration|High Resolution Melting (HRM) Analysis
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher steponeplus real time pcr machine
    The StepOnePlus Real Time PCR System is a 96 well Real Time PCR instrument perfect for both first time and experienced users The StepOnePlus Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 4 color optical recording the StepOnePlus Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications We supply a Dell Laptop with the StepOnePlus Real Time PCR System Features of the StepOnePlus Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays• Sensitive 4 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageNew StepOne SoftwareThe StepOne Software included with the StepOnePlus Real Time PCR System runs on the Windows XP operating system and provides instrument control data collection and data analysis capabilities analysis software is also compatible with Windows 7 This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOnePlus Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 4 Color Fluorescence ReadingThe StepOnePlus Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE NED TAMRA and ROX dyes This cost effective 4 color 96 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus Real Time PCR System and StepOne Software The StepOnePlus System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Protein Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis as a standalone application • High Resolution Melting as a standalone application Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOnePlus System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOnePlus Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOnePlus Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Energy Efficiency and Space SavingThe StepOnePlus Real Time PCR System draws 38 less energy to process one sample plate when the instrument is in a heated state compared to the Bio Rad CFX96 Real Time PCR Detection System The StepOnePlus Real Time PCR System also has advanced temperature control through use of VeriFlex Blocks technology and has a footprint nearly 15 smaller than Bio Rad CFX96 which in today s crowded laboratories helps you use your laboratory space even more efficiently You also leave a smaller environmental footprint on the Earth Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOnePlus Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionAdvanced High Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOnePlus Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate
    https://www.bioz.com/result/steponeplus real time pcr machine/product/Thermo Fisher
    Average 99 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    steponeplus real time pcr machine - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation
    Article Snippet: .. Complementary DNA quantification was performed in duplicate by qRT-PCR using Step-One-Plus Real Time PCR Systems (Applied Biosystems) and normalized against GAPDH expression. ..

    Article Title: Contribution of histone N-terminal tails to the structure and stability of nucleosomes
    Article Snippet: .. The sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems), and the fluorescence signals were measured with this system. .. Since the wavelength at the fluorescence emission maximum of SYPRO Orange is 570 nm, the fluorescence filter ‘filter 3’, which covers the emission wavelength ranges of the TAMRA (580 nm) and NED (575 nm) dyes, was used for detecting the fluorescence of SYPRO Orange bound to the denatured histones.

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
    Article Snippet: .. We use the StepOnePlus™ Real-Time PCR System (Life Technologies) and incorporate the “Fast” setting for all qRT-PCR runs. .. As such, our laboratory incorporates the Fast SYBR® Green Master Mix along with MicroAmp® Fast tubes (all from Life Technologies) for Subheading 3.4, step 1 .

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation
    Article Snippet: .. RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA). ..

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells
    Article Snippet: .. Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies). ..

    Article Title: Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
    Article Snippet: .. We used the TaqMan® Fast Virus 1-Step master mix (Applied Biosystems) for RT-qPCR amplification with the recommended cycling parameters, 50ºC for 5 min for reverse transcription, 95ºC for 20 s for RT inactivation/initial denaturation, followed by 45 cycles of 95ºC for 3 s and 60ºC for 30 s. RNA (2 μL) was used as a template in a 20 µL reaction, and all assays were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). .. The assay amplification efficiency was calculated by the standard curve method ( ) using a 10-fold, 8-log, serial dilution starting at 2 × 108 RNA copies/µL, in duplicate.

    Article Title: VRK3-mediated nuclear localization of HSP70 prevents glutamate excitotoxicity-induced apoptosis and Aβ accumulation via enhancement of ERK phosphatase VHR activity
    Article Snippet: .. Quantitative real-time PCR The mRNA levels of endogenous genes were detected by quantitative real-time PCR using a StepOnePlus Real-Time PCR System (Applied Biosystems) with the FastStart Universal SYBR Green Master (Roche Applied Science). .. A 20 μl of reaction cocktail was constituted of 3 μl diluted cDNA, 10 μl 2X SYBR Green Master Mix and 0.5 μl each of the forward and reverse primers.

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
    Article Snippet: .. The following cycling parameters for PCR amplification are utilized on the StepOnePlus™ Real-Time PCR System (Life Technologies) (with melting curve analysis immediately following in order to confirm the absence of nonspecific amplification): 20 s at 95 °C. .. Using specific software from the qRT-PCR system, assign a baseline range that demonstrates little to no fluorescent activity up to the cycle before amplification becomes evident (usually cycles 3–15).

    Amplification:

    Article Title: Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
    Article Snippet: .. We used the TaqMan® Fast Virus 1-Step master mix (Applied Biosystems) for RT-qPCR amplification with the recommended cycling parameters, 50ºC for 5 min for reverse transcription, 95ºC for 20 s for RT inactivation/initial denaturation, followed by 45 cycles of 95ºC for 3 s and 60ºC for 30 s. RNA (2 μL) was used as a template in a 20 µL reaction, and all assays were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). .. The assay amplification efficiency was calculated by the standard curve method ( ) using a 10-fold, 8-log, serial dilution starting at 2 × 108 RNA copies/µL, in duplicate.

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
    Article Snippet: .. The following cycling parameters for PCR amplification are utilized on the StepOnePlus™ Real-Time PCR System (Life Technologies) (with melting curve analysis immediately following in order to confirm the absence of nonspecific amplification): 20 s at 95 °C. .. Using specific software from the qRT-PCR system, assign a baseline range that demonstrates little to no fluorescent activity up to the cycle before amplification becomes evident (usually cycles 3–15).

    Fluorescence:

    Article Title: Contribution of histone N-terminal tails to the structure and stability of nucleosomes
    Article Snippet: .. The sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems), and the fluorescence signals were measured with this system. .. Since the wavelength at the fluorescence emission maximum of SYPRO Orange is 570 nm, the fluorescence filter ‘filter 3’, which covers the emission wavelength ranges of the TAMRA (580 nm) and NED (575 nm) dyes, was used for detecting the fluorescence of SYPRO Orange bound to the denatured histones.

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells
    Article Snippet: .. Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies). ..

    Quantitative RT-PCR:

    Article Title: Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation
    Article Snippet: .. Complementary DNA quantification was performed in duplicate by qRT-PCR using Step-One-Plus Real Time PCR Systems (Applied Biosystems) and normalized against GAPDH expression. ..

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
    Article Snippet: .. We use the StepOnePlus™ Real-Time PCR System (Life Technologies) and incorporate the “Fast” setting for all qRT-PCR runs. .. As such, our laboratory incorporates the Fast SYBR® Green Master Mix along with MicroAmp® Fast tubes (all from Life Technologies) for Subheading 3.4, step 1 .

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation
    Article Snippet: .. RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA). ..

    Article Title: Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
    Article Snippet: .. We used the TaqMan® Fast Virus 1-Step master mix (Applied Biosystems) for RT-qPCR amplification with the recommended cycling parameters, 50ºC for 5 min for reverse transcription, 95ºC for 20 s for RT inactivation/initial denaturation, followed by 45 cycles of 95ºC for 3 s and 60ºC for 30 s. RNA (2 μL) was used as a template in a 20 µL reaction, and all assays were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). .. The assay amplification efficiency was calculated by the standard curve method ( ) using a 10-fold, 8-log, serial dilution starting at 2 × 108 RNA copies/µL, in duplicate.

    SYBR Green Assay:

    Article Title: VRK3-mediated nuclear localization of HSP70 prevents glutamate excitotoxicity-induced apoptosis and Aβ accumulation via enhancement of ERK phosphatase VHR activity
    Article Snippet: .. Quantitative real-time PCR The mRNA levels of endogenous genes were detected by quantitative real-time PCR using a StepOnePlus Real-Time PCR System (Applied Biosystems) with the FastStart Universal SYBR Green Master (Roche Applied Science). .. A 20 μl of reaction cocktail was constituted of 3 μl diluted cDNA, 10 μl 2X SYBR Green Master Mix and 0.5 μl each of the forward and reverse primers.

    Expressing:

    Article Title: Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation
    Article Snippet: .. Complementary DNA quantification was performed in duplicate by qRT-PCR using Step-One-Plus Real Time PCR Systems (Applied Biosystems) and normalized against GAPDH expression. ..

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells
    Article Snippet: .. Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies). ..

    Polymerase Chain Reaction:

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
    Article Snippet: .. The following cycling parameters for PCR amplification are utilized on the StepOnePlus™ Real-Time PCR System (Life Technologies) (with melting curve analysis immediately following in order to confirm the absence of nonspecific amplification): 20 s at 95 °C. .. Using specific software from the qRT-PCR system, assign a baseline range that demonstrates little to no fluorescent activity up to the cycle before amplification becomes evident (usually cycles 3–15).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher abi steponeplus real time pcr machine
    Abi Steponeplus Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus real time pcr machine/product/Thermo Fisher
    Average 99 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus real time pcr machine - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results