stepone realtime pcr systems  (Thermo Fisher)


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    Name:
    StepOne Real Time PCR System
    Description:
    The StepOne Real Time PCR System is a 48 well low throughput Real Time PCR instrument perfect for both first time and experienced users The StepOne Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 3 color optical recording the StepOne Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications Features of the The StepOne Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays • Sensitive 3 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageStepOne SoftwareThe StepOne Software included with the StepOne Real Time PCR System runs on Windows XP Window Vista and Windows 7 operating systems and provides instrument control data collection and data analysis capabilities This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOne Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 3 Color Fluorescence ReadingThe StepOne Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE and ROX dyes This cost effective 3 color 48 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne Real Time PCR System and software The StepOne System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis• High Resolution Melting additional software required Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOne System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOne Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOne Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOne Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionHigh Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOne Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4376357
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Gene Expression Analysis & Genotyping|Genotyping Instruments, Software & Calibration|High Resolution Melting (HRM) Analysis
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher stepone realtime pcr systems
    The StepOne Real Time PCR System is a 48 well low throughput Real Time PCR instrument perfect for both first time and experienced users The StepOne Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 3 color optical recording the StepOne Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications Features of the The StepOne Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays • Sensitive 3 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageStepOne SoftwareThe StepOne Software included with the StepOne Real Time PCR System runs on Windows XP Window Vista and Windows 7 operating systems and provides instrument control data collection and data analysis capabilities This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOne Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 3 Color Fluorescence ReadingThe StepOne Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE and ROX dyes This cost effective 3 color 48 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne Real Time PCR System and software The StepOne System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis• High Resolution Melting additional software required Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOne System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOne Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOne Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOne Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionHigh Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOne Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/stepone realtime pcr systems/product/Thermo Fisher
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    stepone realtime pcr systems - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Suitability of Nicotinic Acetylcholine Receptor α7 and Muscarinic Acetylcholine Receptor 3 Antibodies for Immune Detection
    Article Snippet: .. Equal amounts of RNA were reverse transcribed with Superscript II reverse transcriptase (Life Technologies) for 50 min at 42°C. cDNA was then subjected to qRT-PCR analysis (StepOne Real-time PCR system, Life Technologies) using QuantiTect Probe PCR kit (Qiagen) and two sets of specific forward and reverse primers for Chrna7 spanning exons 1–4 and exons 8–10. .. Gene expression was normalized to HPRT calculating the difference between the cycle time (CT ) for HPRT and the CT for the respective transcript.

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays
    Article Snippet: .. The reactions were carried out on a StepOne™ Real-Time PCR System (Applied Biosystems® —Thermo Fisher Scientific), by using the StepOne Software (Applied Biosystems® —Thermo Fisher Scientific). .. After a first 2-minute cycle at 50°C and a second 10-minute cycle at 95°C, the amplification cycles were different for each specific genotyping assay: 50 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 62°C for 1 minute for β0 39, β+ IVSI-110 and β+ IVSI-6 assays; 60 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 60°C for 1 minute for β0 IVSI-1.

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Article Title: Role of PU.1 in MHC Class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells
    Article Snippet: .. The mRNA levels of PU.1, HLA-DRα and mouse MHC class II, CIITA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantified by using a Step-One Real-Time PCR system (Applied Biosystems) with TaqMan Gene Expression Assays (Applied Biosystems: no. Hs02786711_m1 for human PU.1, Mm01270606_m1 for mouse PU.1, Hs00219575_m1 for HLA-DRα, Mm00772352_m1 for I-Eα, Hs00172106_m1 for human CIITA, Mm00482919_m1 for mouse CIITA, Mm01342720_m1 for mouse CIITA mRNA driven from promoter III (pIII-CIITA), human GAPDH no. 4326317E, and mouse GAPDH no. 4352339E) and TaqMan Universal Master Mix (Applied Biosystems). .. For the measurement of human pIII-CIITA, the following primers and probe were originally constructed using the customized service of Applied Biosystems: forward primer, 5’-GCTGGGATTCCTACACAATGC-3’; reverse primer, 5’-TCTCCAGCCAGGTCCATCTG-3’; and probe, 5’-FAM-CCCAAGGCAGCTCA-MGB-3’.

    Article Title: Expression of Glycogen synthase kinase 3-? (GSK3-?) gene in azoospermic men
    Article Snippet: .. Subsequent real time PCR was done with Power Syber Green real time master mix and Stepone real time PCR (Applied biosystems, Carlsbad, USA). ..

    Quantitative RT-PCR:

    Article Title: Suitability of Nicotinic Acetylcholine Receptor α7 and Muscarinic Acetylcholine Receptor 3 Antibodies for Immune Detection
    Article Snippet: .. Equal amounts of RNA were reverse transcribed with Superscript II reverse transcriptase (Life Technologies) for 50 min at 42°C. cDNA was then subjected to qRT-PCR analysis (StepOne Real-time PCR system, Life Technologies) using QuantiTect Probe PCR kit (Qiagen) and two sets of specific forward and reverse primers for Chrna7 spanning exons 1–4 and exons 8–10. .. Gene expression was normalized to HPRT calculating the difference between the cycle time (CT ) for HPRT and the CT for the respective transcript.

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    TaqMan microRNA Assay:

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    SYBR Green Assay:

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Polymerase Chain Reaction:

    Article Title: Suitability of Nicotinic Acetylcholine Receptor α7 and Muscarinic Acetylcholine Receptor 3 Antibodies for Immune Detection
    Article Snippet: .. Equal amounts of RNA were reverse transcribed with Superscript II reverse transcriptase (Life Technologies) for 50 min at 42°C. cDNA was then subjected to qRT-PCR analysis (StepOne Real-time PCR system, Life Technologies) using QuantiTect Probe PCR kit (Qiagen) and two sets of specific forward and reverse primers for Chrna7 spanning exons 1–4 and exons 8–10. .. Gene expression was normalized to HPRT calculating the difference between the cycle time (CT ) for HPRT and the CT for the respective transcript.

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Expressing:

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: Role of PU.1 in MHC Class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells
    Article Snippet: .. The mRNA levels of PU.1, HLA-DRα and mouse MHC class II, CIITA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantified by using a Step-One Real-Time PCR system (Applied Biosystems) with TaqMan Gene Expression Assays (Applied Biosystems: no. Hs02786711_m1 for human PU.1, Mm01270606_m1 for mouse PU.1, Hs00219575_m1 for HLA-DRα, Mm00772352_m1 for I-Eα, Hs00172106_m1 for human CIITA, Mm00482919_m1 for mouse CIITA, Mm01342720_m1 for mouse CIITA mRNA driven from promoter III (pIII-CIITA), human GAPDH no. 4326317E, and mouse GAPDH no. 4352339E) and TaqMan Universal Master Mix (Applied Biosystems). .. For the measurement of human pIII-CIITA, the following primers and probe were originally constructed using the customized service of Applied Biosystems: forward primer, 5’-GCTGGGATTCCTACACAATGC-3’; reverse primer, 5’-TCTCCAGCCAGGTCCATCTG-3’; and probe, 5’-FAM-CCCAAGGCAGCTCA-MGB-3’.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antioxidant effects of fucoxanthin rich powder in rats fed with high fat diet
    Article Snippet: .. Ct-values of target genes and the reference gene were obtained using an Applied Biosystems StepOne Plus RT-PCR system (Applied biosystems, CA, USA). ..

    Software:

    Article Title: Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays
    Article Snippet: .. The reactions were carried out on a StepOne™ Real-Time PCR System (Applied Biosystems® —Thermo Fisher Scientific), by using the StepOne Software (Applied Biosystems® —Thermo Fisher Scientific). .. After a first 2-minute cycle at 50°C and a second 10-minute cycle at 95°C, the amplification cycles were different for each specific genotyping assay: 50 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 62°C for 1 minute for β0 39, β+ IVSI-110 and β+ IVSI-6 assays; 60 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 60°C for 1 minute for β0 IVSI-1.

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  • 99
    Thermo Fisher stepone plus realtime pcr system
    Stepone Plus Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stepone plus realtime pcr system/product/Thermo Fisher
    Average 99 stars, based on 1834 article reviews
    Price from $9.99 to $1999.99
    stepone plus realtime pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher stepone realtime pcr system
    Stepone Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stepone realtime pcr system/product/Thermo Fisher
    Average 88 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    stepone realtime pcr system - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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