stat5a sc-1081 antibody  (Santa Cruz Biotechnology)

Journal: Nature

Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness

doi: 10.1038/s41586-021-03861-0

Figure Lengend Snippet: a, ChIP-seq analysis of STAT5A and STAT5B binding sites at the Havcr2 locus from previously published datasets (GSE36890).

Article Snippet: Anti-STAT5A (sc-1081, 1:1000) and anti-STAT5B (Sc-1656, 1:1000) were from Santa Cruz.

Techniques: Activation Assay, ChIP-sequencing, Binding Assay

Journal: Cell reports

Article Title: A Spontaneous Aggressive ERα+ Mammary Tumor Model Is Driven by Kras Activation

doi: 10.1016/j.celrep.2019.06.098

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mammary structures were assessed in hematoxylin and eosin (H&E) stained tissue sections, and protein expression was visualized by immunohistochemistry as previously described ( Arendt et al., 2011 ) using antibodies against the following antigens: Cyclin D1, CP236B, Biocare Medical, (1:200); ER, #SC-542, Santa Cruz Biotechnology (1:500); PR, A0098, Dako (1:500); pAKT S473 , #3787, Cell Signaling Technologies (1:50); pERK1/2, #9101, Cell Signaling Technologies (1:400); STAT5A, #SC-1081, Santa Cruz Biotechnology (1:5000).

Techniques: Cell Culture, Purification, SYBR Green Assay, Sequencing, Real-time Polymerase Chain Reaction, Software

Journal: American Journal of Cancer Research

Article Title: MiRNA-1469 promotes lung cancer cells apoptosis through targeting STAT5a

doi:

Figure Lengend Snippet: Stat5a is a direct target of miRNA-1469. A. MiR-1469 targeting site resides at nucleotides 226-232 of Stat5a-3’UTR and is highly conserved in different species. Upper panel: sequence alignment of miR-1469 with binding sites on the Stat5a-3’UTR. Bottom panel: sequence of the miR-1469 binding site within the Stat5a-3’UTR of three species (human, chimpanzee and rhesus). B. Diagram of the luciferase reporter plasmids including plasmid with the full-length Stat5a-3’UTR insert (pIS0-Stat5a-3’UTR) and plasmid with a mutant Stat5a-3’UTR (pIS0-Stat5a-3’UTR-mut) which carried a substitution of three nucleotides within the miR-1469 binding site. C. Luciferase activity assay demonstrates a direct targeting of the Stat5a-3’UTR by miR-1469. A549 cells (left panel) and H1650 cells (right panel) were transfected with miR-1469 mimics (20 nM) and pIS0-Stat5a-3’UTR /pIS0-Stat5a-3’UTR-mut. pRL-SV40 Renilla was used for the normalization of transfection efficiency. After 48 h, the luciferase activities were measured. D. Western blotting was used to detect the expression of the Stat5a protein after miRNA-1469 mimics (20 nM) or miRNA-1469; Inhibitor (40 nM) transfection of A549 (left panel) or H1650 (right panel) cells. E. Stat5a mRNA in the A549 (left panel) or H1650 (right panel) cell lines treated as above was measured with real-time RT-PCR. β-actin was used as internal control. F. Overexpression of miR-1469 mimics (20 nM) or miRNA-1469.Inhibitor (40 nM) in A549 or H1650 cell lines transfected miRNA-1469 mimics or miRNA-1469. Inhibitor was measured by real-time RT-PCR. U6 was used as internal control. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: The antibodies against Stat5a (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-1081), phosphorylated H2AX (gH2AX) (Cell Signaling Technology, Beverly, MA, USA, # 9718), Bcl-2 and b-actin (Cell Signaling Technology, #4970) were respectively used to detect their targeting proteins: Statistical analysis Data were presented as mean±SD from at least three separate experiments, and Student’s t-test analysis was performed using SPSS 17.0 software.

Techniques: Sequencing, Binding Assay, Luciferase, Plasmid Preparation, Mutagenesis, Activity Assay, Transfection, Western Blot, Expressing, Quantitative RT-PCR, Control, Over Expression

Journal: American Journal of Cancer Research

Article Title: MiRNA-1469 promotes lung cancer cells apoptosis through targeting STAT5a

doi:

Figure Lengend Snippet: MiR-1469 regulates apoptosis through Stat5a. A. Apoptosis of A549 cell were detected by FCM 48 h after miRNA-1469 mimics and combined with Stat5a transfection (left panel). Error bars denote mean ± SD (right panel). B. The apoptosis of H1650 cells 48 h after transfection of miRNA-1469 mimics and combined with Stat5a were detected by FCM (left panel). Error bars denote mean ± SD (right panel). **, p < 0.01; ***, p < 0.001.

Article Snippet: The antibodies against Stat5a (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-1081), phosphorylated H2AX (gH2AX) (Cell Signaling Technology, Beverly, MA, USA, # 9718), Bcl-2 and b-actin (Cell Signaling Technology, #4970) were respectively used to detect their targeting proteins: Statistical analysis Data were presented as mean±SD from at least three separate experiments, and Student’s t-test analysis was performed using SPSS 17.0 software.

Techniques: Transfection