staphylococcus aureus mnase  (Millipore)


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  • 99
    Name:
    Staphylococcus aureus
    Description:

    Catalog Number:
    s2014
    Price:
    None
    Applications:
    Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.
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    Structured Review

    Millipore staphylococcus aureus mnase

    https://www.bioz.com/result/staphylococcus aureus mnase/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    staphylococcus aureus mnase - by Bioz Stars, 2020-08
    99/100 stars

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    micrococcal nuclease

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    Related Articles

    Clone Assay:

    Article Title: Identification and in vitro Analysis of the GatD/MurT Enzyme-Complex Catalyzing Lipid II Amidation in Staphylococcus aureusIdentification of Genetic Determinants and Enzymes Involved with the Amidation of Glutamic Acid Residues in the Peptidoglycan of Staphylococcus aureus
    Article Snippet: .. Cloning, overexpression and purification of S. aureus murT , gatD and glnA as His6-tag fusions S. aureus N315 murT (SA1708), gatD (SA1707) and glnA were amplified using forward and reverse primers as listed in and cloned into a pET21b vector (Novagen) using NdeI and XhoI restriction sites to generate C-terminal His6 -fusion proteins. .. E. coli BL21(DE3) (Promega) cells transformed with the appropriate recombinant plasmid were grown in LB-medium (50 µg/ml ampicillin) at 37°C.

    RNA Extraction:

    Article Title: Transcriptomics Study on Staphylococcus aureus Biofilm Under Low Concentration of Ampicillin
    Article Snippet: .. Total RNA of S. aureus biofilm samples (A1, A2, A3, C1, C2, and C3) were extracted using the TRizol reagent (Sigma-Aldrich, United States) and Bacterial Total RNA Extraction kit (Sigma-Aldrich, United States) based on the manufacturer’s instruction ( , ). .. The quality and quantity of the RNA samples were determined by Ultramicro spectrophotometer K5500 (Beijing Kaiao Technology Development Co., Ltd., China) and Agilent 2200 TapStation (Agilent Technologies, United States).

    Amplification:

    Article Title: Identification and in vitro Analysis of the GatD/MurT Enzyme-Complex Catalyzing Lipid II Amidation in Staphylococcus aureusIdentification of Genetic Determinants and Enzymes Involved with the Amidation of Glutamic Acid Residues in the Peptidoglycan of Staphylococcus aureus
    Article Snippet: .. Cloning, overexpression and purification of S. aureus murT , gatD and glnA as His6-tag fusions S. aureus N315 murT (SA1708), gatD (SA1707) and glnA were amplified using forward and reverse primers as listed in and cloned into a pET21b vector (Novagen) using NdeI and XhoI restriction sites to generate C-terminal His6 -fusion proteins. .. E. coli BL21(DE3) (Promega) cells transformed with the appropriate recombinant plasmid were grown in LB-medium (50 µg/ml ampicillin) at 37°C.

    Isolation:

    Article Title: O-Acetylation is essential for functional antibody generation against Staphylococcus aureus capsular polysaccharide
    Article Snippet: .. Production of de-O-Acetylated S. aureus capsular polysaccharide conjugates CP8 and CP5 were isolated as previously described from CP5 expresing strain BD285 and CP8 expressing strain BD287, then de-O-acetylated by incubation in 0.2 N NaOH or 0.2 N TBOH, respectively (Sigma) at 37ºC for 4 h. Samples were neutralized with HCl, washed with water, and resuspended at ∼4 mg/mL as determined with 4-hydroxybenzoic acid hydrazide (PAHBAH). .. Removal of O-acetyl groups was verified by size exclusion chromatography multi angle laser light scattering (SEC MALLS).

    Purification:

    Article Title: Membrane-Active Epithelial Keratin 6A Fragments (KAMPs) Are Unique Human Antimicrobial Peptides with a Non-αβ Structure
    Article Snippet: .. Purified P. aeruginosa LPS and S. aureus LTA (Sigma-Aldrich) were prepared in distilled water. .. Colistin and meropenem (Santa Cruz Biotechnology) were used as positive and negative controls respectively (Ouberai et al., ).

    Article Title: Identification and in vitro Analysis of the GatD/MurT Enzyme-Complex Catalyzing Lipid II Amidation in Staphylococcus aureusIdentification of Genetic Determinants and Enzymes Involved with the Amidation of Glutamic Acid Residues in the Peptidoglycan of Staphylococcus aureus
    Article Snippet: .. Cloning, overexpression and purification of S. aureus murT , gatD and glnA as His6-tag fusions S. aureus N315 murT (SA1708), gatD (SA1707) and glnA were amplified using forward and reverse primers as listed in and cloned into a pET21b vector (Novagen) using NdeI and XhoI restriction sites to generate C-terminal His6 -fusion proteins. .. E. coli BL21(DE3) (Promega) cells transformed with the appropriate recombinant plasmid were grown in LB-medium (50 µg/ml ampicillin) at 37°C.

    Incubation:

    Article Title: O-Acetylation is essential for functional antibody generation against Staphylococcus aureus capsular polysaccharide
    Article Snippet: .. Production of de-O-Acetylated S. aureus capsular polysaccharide conjugates CP8 and CP5 were isolated as previously described from CP5 expresing strain BD285 and CP8 expressing strain BD287, then de-O-acetylated by incubation in 0.2 N NaOH or 0.2 N TBOH, respectively (Sigma) at 37ºC for 4 h. Samples were neutralized with HCl, washed with water, and resuspended at ∼4 mg/mL as determined with 4-hydroxybenzoic acid hydrazide (PAHBAH). .. Removal of O-acetyl groups was verified by size exclusion chromatography multi angle laser light scattering (SEC MALLS).

    other:

    Article Title: Staphylococcus aureus Protein A Recognizes Platelet gC1qR/p33: a Novel Mechanism for Staphylococcal Interactions with Platelets
    Article Snippet: The following chemicals and reagents were purchased from the sources indicated: protein A (Cowan I), human complement component C1q, bovine serum albumin (BSA), rabbit IgG, p -nitrophenyl phosphate (pNPP), S. aureus Cowan I and Wood 46 strains (formalin-treated cell suspensions), and dimethyl sulfoxide (DMSO), Sigma Chemical Co. (St. Louis, Mo.); alkaline phosphatase-conjugated goat anti-rabbit IgG, Organon Teknika Corp. (West Chester, Pa.); alkaline phosphatase-conjugated streptavidin (AP-STRAV), protein A-agarose, and sulfosuccinimidyl-6-biotinamidohexanoate (NHS-LC-biotin), Pierce Chemical Co. (Rockford, Ill.); Tween 20, J. T. Baker (Phillipsburg, N.J.); PD-10 columns, Pharmacia Biotech (Piscataway, N.J.).

    Fractionation:

    Article Title: Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models
    Article Snippet: .. In order to perform proteomic analysis, fractionation of MVs from S. aureus MSSA476 grown in LB and BHI broth was carried out by density gradient centrifugation using Optiprep (Sigma–Aldrich, Germany) as previously described , with minor modifications. ..

    Expressing:

    Article Title: O-Acetylation is essential for functional antibody generation against Staphylococcus aureus capsular polysaccharide
    Article Snippet: .. Production of de-O-Acetylated S. aureus capsular polysaccharide conjugates CP8 and CP5 were isolated as previously described from CP5 expresing strain BD285 and CP8 expressing strain BD287, then de-O-acetylated by incubation in 0.2 N NaOH or 0.2 N TBOH, respectively (Sigma) at 37ºC for 4 h. Samples were neutralized with HCl, washed with water, and resuspended at ∼4 mg/mL as determined with 4-hydroxybenzoic acid hydrazide (PAHBAH). .. Removal of O-acetyl groups was verified by size exclusion chromatography multi angle laser light scattering (SEC MALLS).

    Gradient Centrifugation:

    Article Title: Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models
    Article Snippet: .. In order to perform proteomic analysis, fractionation of MVs from S. aureus MSSA476 grown in LB and BHI broth was carried out by density gradient centrifugation using Optiprep (Sigma–Aldrich, Germany) as previously described , with minor modifications. ..

    Activity Assay:

    Article Title: In Silico Discovery of a Substituted 6-Methoxy-quinalidine with Leishmanicidal Activity in Leishmania infantum
    Article Snippet: .. Steady-State Kinetics Experiments to Determine Inhibition Activity of NDH-2 from S. aureus NADH was purchased from Sigma Aldrich. .. 2,3-Dimethyl1,4-naphthoquinone (DMN) was synthesized from menadione (Sigma Aldrich, St. Louis, MO, USA) [ ].

    Over Expression:

    Article Title: Identification and in vitro Analysis of the GatD/MurT Enzyme-Complex Catalyzing Lipid II Amidation in Staphylococcus aureusIdentification of Genetic Determinants and Enzymes Involved with the Amidation of Glutamic Acid Residues in the Peptidoglycan of Staphylococcus aureus
    Article Snippet: .. Cloning, overexpression and purification of S. aureus murT , gatD and glnA as His6-tag fusions S. aureus N315 murT (SA1708), gatD (SA1707) and glnA were amplified using forward and reverse primers as listed in and cloned into a pET21b vector (Novagen) using NdeI and XhoI restriction sites to generate C-terminal His6 -fusion proteins. .. E. coli BL21(DE3) (Promega) cells transformed with the appropriate recombinant plasmid were grown in LB-medium (50 µg/ml ampicillin) at 37°C.

    Inhibition:

    Article Title: In Silico Discovery of a Substituted 6-Methoxy-quinalidine with Leishmanicidal Activity in Leishmania infantum
    Article Snippet: .. Steady-State Kinetics Experiments to Determine Inhibition Activity of NDH-2 from S. aureus NADH was purchased from Sigma Aldrich. .. 2,3-Dimethyl1,4-naphthoquinone (DMN) was synthesized from menadione (Sigma Aldrich, St. Louis, MO, USA) [ ].

    Plasmid Preparation:

    Article Title: Identification and in vitro Analysis of the GatD/MurT Enzyme-Complex Catalyzing Lipid II Amidation in Staphylococcus aureusIdentification of Genetic Determinants and Enzymes Involved with the Amidation of Glutamic Acid Residues in the Peptidoglycan of Staphylococcus aureus
    Article Snippet: .. Cloning, overexpression and purification of S. aureus murT , gatD and glnA as His6-tag fusions S. aureus N315 murT (SA1708), gatD (SA1707) and glnA were amplified using forward and reverse primers as listed in and cloned into a pET21b vector (Novagen) using NdeI and XhoI restriction sites to generate C-terminal His6 -fusion proteins. .. E. coli BL21(DE3) (Promega) cells transformed with the appropriate recombinant plasmid were grown in LB-medium (50 µg/ml ampicillin) at 37°C.

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  • 99
    Millipore nuclease micrococcal from staphylococcus aureus
    Nuclease Micrococcal From Staphylococcus Aureus, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease micrococcal from staphylococcus aureus/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nuclease micrococcal from staphylococcus aureus - by Bioz Stars, 2020-08
    99/100 stars
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