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MACHEREY NAGEL standard miniprep kit macherey nagel
Standard Miniprep Kit Macherey Nagel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard miniprep kit macherey nagel/product/MACHEREY NAGEL
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
standard miniprep kit macherey nagel - by Bioz Stars, 2020-04
89/100 stars

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DNA Ligation:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

Mutagenesis:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: .. 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

Infection:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: .. 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

Purification:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: .. 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

Polymerase Chain Reaction:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

Sequencing:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. The presence of each substitution was confirmed by Sanger sequencing.

Lysis:

Article Title: Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
Article Snippet: .. 2.2 Site-directed mutagenesis The ϕX174 dsDNA replicative form was purified from infected cultures before lysis using a standard miniprep kit (Macherey-Nagel), and 500 pg of this DNA were used as template for polymerase chain reaction-based mutagenesis using Phusion high-fidelity DNA polymerase (Thermo Scientific) and contiguous, divergent, 5’-phosphorylated primers, of which the reverse primer carried the desired nucleotide substitution. .. Polymerase chain reaction products were circularized with the Rapid DNA ligation kit (Thermo Scientific) and used for transfecting competent IJ1862cells by the classical heat-shock method.

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    MACHEREY NAGEL standard miniprep kit macherey nagel
    Standard Miniprep Kit Macherey Nagel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard miniprep kit macherey nagel/product/MACHEREY NAGEL
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard miniprep kit macherey nagel - by Bioz Stars, 2020-04
    89/100 stars
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