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Illumina Inc standard illumina truseq multiplexing oligos
Standard Illumina Truseq Multiplexing Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard illumina truseq multiplexing oligos/product/Illumina Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
standard illumina truseq multiplexing oligos - by Bioz Stars, 2020-08
92/100 stars

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Polymerase Chain Reaction:

Article Title: Human genome-wide measurement of drug-responsive regulatory activity
Article Snippet: .. Following enzyme inactivation, cDNAs were treated with RNase A (Sigma) at 37 °C for 1 h, purified with SPRI beads (2.0×), and subjected to PCR enrichment with reporter-specific primer as perviously described under the following conditions: 98 °C for 30 s, followed by 6 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were purified with SPRI beads (1.5×) and amplified again by PCR using a standard Illumina TruSeq multiplexing oligos under the following conditions: 98 °C for 30 s, followed by 10 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. .. Libraries from matched samples were pooled and assessed by sequencing on an Illumina MiSeq, as above, using 50 bp paired-end reads prior to deep sequencing on the Illumina HiSeq 4000.

Multiplexing:

Article Title: Human genome-wide measurement of drug-responsive regulatory activity
Article Snippet: .. Following enzyme inactivation, cDNAs were treated with RNase A (Sigma) at 37 °C for 1 h, purified with SPRI beads (2.0×), and subjected to PCR enrichment with reporter-specific primer as perviously described under the following conditions: 98 °C for 30 s, followed by 6 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were purified with SPRI beads (1.5×) and amplified again by PCR using a standard Illumina TruSeq multiplexing oligos under the following conditions: 98 °C for 30 s, followed by 10 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. .. Libraries from matched samples were pooled and assessed by sequencing on an Illumina MiSeq, as above, using 50 bp paired-end reads prior to deep sequencing on the Illumina HiSeq 4000.

Amplification:

Article Title: Human genome-wide measurement of drug-responsive regulatory activity
Article Snippet: .. Following enzyme inactivation, cDNAs were treated with RNase A (Sigma) at 37 °C for 1 h, purified with SPRI beads (2.0×), and subjected to PCR enrichment with reporter-specific primer as perviously described under the following conditions: 98 °C for 30 s, followed by 6 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were purified with SPRI beads (1.5×) and amplified again by PCR using a standard Illumina TruSeq multiplexing oligos under the following conditions: 98 °C for 30 s, followed by 10 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. .. Libraries from matched samples were pooled and assessed by sequencing on an Illumina MiSeq, as above, using 50 bp paired-end reads prior to deep sequencing on the Illumina HiSeq 4000.

Purification:

Article Title: Human genome-wide measurement of drug-responsive regulatory activity
Article Snippet: .. Following enzyme inactivation, cDNAs were treated with RNase A (Sigma) at 37 °C for 1 h, purified with SPRI beads (2.0×), and subjected to PCR enrichment with reporter-specific primer as perviously described under the following conditions: 98 °C for 30 s, followed by 6 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were purified with SPRI beads (1.5×) and amplified again by PCR using a standard Illumina TruSeq multiplexing oligos under the following conditions: 98 °C for 30 s, followed by 10 cycles of 98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s, with a final extension at 72 °C for 5 min. .. Libraries from matched samples were pooled and assessed by sequencing on an Illumina MiSeq, as above, using 50 bp paired-end reads prior to deep sequencing on the Illumina HiSeq 4000.

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    Illumina Inc standard illumina truseq multiplexing oligos
    Standard Illumina Truseq Multiplexing Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard illumina truseq multiplexing oligos/product/Illumina Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard illumina truseq multiplexing oligos - by Bioz Stars, 2020-08
    92/100 stars
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