standard desalted ultramer oligonucleotides  (Integrated DNA Technologies)


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    Name:
    Ultramer DNA Oligos
    Description:
    Long high quality oligos for demanding applications such as cloning ddRNAi homology directed repair and gene construction Generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases Available single or double stranded and can be delivered in tubes or plates
    Catalog Number:
    udo-605976
    Price:
    None
    Category:
    Nucleic acids
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    Structured Review

    Integrated DNA Technologies standard desalted ultramer oligonucleotides
    Long high quality oligos for demanding applications such as cloning ddRNAi homology directed repair and gene construction Generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases Available single or double stranded and can be delivered in tubes or plates
    https://www.bioz.com/result/standard desalted ultramer oligonucleotides/product/Integrated DNA Technologies
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    standard desalted ultramer oligonucleotides - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    IA:

    Article Title: Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells
    Article Snippet: .. Coligo synthesis Synthetic DNA Ultramer® oligonucleotides with 5′ phosphorylation were purchased from Integrated DNA Technologies (Coralville, IA, USA). .. Enzymatic DNA cyclization using the TS2126 RNA ligase, coligo purification, RNA marker, denaturing polyacrylamide gel electrophoresis (DPAGE) were done as previously described ( ).

    Positive Control:

    Article Title: Viral, bacterial, and protozoan pathogens and fecal markers in wells supplying groundwater to public water systems in Minnesota, USA.
    Article Snippet: .. Drinking water supply wells can be contaminated by a broad range of waterborne pathogens. ..

    Ligation:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

    Synthesized:

    Article Title: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Article Snippet: .. Paired sgRNAs were designed for the ACTB (actin beta, Homo sapiens ; PubMed Gene ID: 60) target gene locus using an online CRISPR gRNA design tool , and synthesized as Ultramer DNA Oligonucleotides (Integrated DNA Technologies), ACTB _sgRNA and ACTB _HDR (Supplementary Table ). .. Next, paired sgRNAs were cloned according to a previously described procedure into a plasmid harboring Cas9D10A and a puromycin resistance cassette .

    Article Title: A 5′ Nuclease Genotyping Assay for Identification of Macrolide-Resistant Mycoplasma genitalium in Clinical Specimens
    Article Snippet: .. As in-run controls, synthesized Ultramer oligonucleotides (Integrated DNA Technologies, Leuven, Belgium) comprising the entire PCR product were used. .. A no-template control was included in each PCR run.

    Construct:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

    Article Title: Synthetic feedback control using an RNAi-based gene-regulatory device
    Article Snippet: .. All miRNA and ribozyme components were constructed by ordering two ultramer oligonucleotides (Integrated DNA Technologies), which were reverse complements of each other at their 3′ ends. .. The oligonucleotide pairs were subjected to five rounds of annealing and extension using Taq polymerase (New England Biolabs) in a thermocycler.

    Real-time Polymerase Chain Reaction:

    Article Title: Viral, bacterial, and protozoan pathogens and fecal markers in wells supplying groundwater to public water systems in Minnesota, USA.
    Article Snippet: .. Drinking water supply wells can be contaminated by a broad range of waterborne pathogens. ..

    Polymerase Chain Reaction:

    Article Title: A 5′ Nuclease Genotyping Assay for Identification of Macrolide-Resistant Mycoplasma genitalium in Clinical Specimens
    Article Snippet: .. As in-run controls, synthesized Ultramer oligonucleotides (Integrated DNA Technologies, Leuven, Belgium) comprising the entire PCR product were used. .. A no-template control was included in each PCR run.

    CRISPR:

    Article Title: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Article Snippet: .. Paired sgRNAs were designed for the ACTB (actin beta, Homo sapiens ; PubMed Gene ID: 60) target gene locus using an online CRISPR gRNA design tool , and synthesized as Ultramer DNA Oligonucleotides (Integrated DNA Technologies), ACTB _sgRNA and ACTB _HDR (Supplementary Table ). .. Next, paired sgRNAs were cloned according to a previously described procedure into a plasmid harboring Cas9D10A and a puromycin resistance cassette .

    Modification:

    Article Title: Viral, bacterial, and protozoan pathogens and fecal markers in wells supplying groundwater to public water systems in Minnesota, USA.
    Article Snippet: .. Drinking water supply wells can be contaminated by a broad range of waterborne pathogens. ..

    Plasmid Preparation:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

    Article Title: Organoid Easytag: an efficient workflow for gene targeting in human organoids
    Article Snippet: .. 4 × 105 cells were suspended using Lonza Nucleofection P3 buffer, mixed with 10 µg of appropriate plasmid repair template, or with 500 pmol ACTB-GFP11 ssODN (Ultramer DNA Oligos, IDT) with 5 µg CMV-GFP(1-10) plasmid. .. The cell suspension was further mixed with pre-formed Cas9 RNPs and equally distributed into two 20 µl cuvettes (V4XP-3024, Lonza).

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  • 99
    Integrated DNA Technologies standard desalted ultramer oligonucleotides
    Standard Desalted Ultramer Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard desalted ultramer oligonucleotides/product/Integrated DNA Technologies
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    standard desalted ultramer oligonucleotides - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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