standard desalted ultramer oligonucleotides  (Integrated DNA Technologies)


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    Name:
    Ultramer DNA Oligos
    Description:
    Long high quality oligos for demanding applications such as cloning ddRNAi homology directed repair and gene construction Generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases Available single or double stranded and can be delivered in tubes or plates
    Catalog Number:
    udo-605976
    Price:
    None
    Category:
    Nucleic acids
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    Structured Review

    Integrated DNA Technologies standard desalted ultramer oligonucleotides
    Long high quality oligos for demanding applications such as cloning ddRNAi homology directed repair and gene construction Generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases Available single or double stranded and can be delivered in tubes or plates
    https://www.bioz.com/result/standard desalted ultramer oligonucleotides/product/Integrated DNA Technologies
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    standard desalted ultramer oligonucleotides - by Bioz Stars, 2020-08
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Id genes are essential for early heart formation
    Article Snippet: .. DNA templates for sgRNAs were generated by PCR amplification (Phusion DNA polymerase, New England Biolabs) of ssDNA ultramer oligonucleotides (Integrated DNA Technologies) (see the ); sgRNAs were transcribed from these templates using HiScribe T7 high-yield RNA synthesis kit (New England Biolabs) and purified using Megaclear kit (Life Technologies). .. For mouse zygote injections, 50 ng/µL Cas9 mRNA (Life Technologies) and 20 ng/µL each sgRNA were combined in nuclease-free water.

    IA:

    Article Title: Development of a Real-Time Reverse Transcription Polymerase Chain Reaction for O’nyong-nyong Virus and Evaluation with Clinical and Mosquito Specimens from Kenya
    Article Snippet: .. Linear range, precision, and lower limit of 95% detection experiments were performed using synthesized Ultramer Oligos (Integrated DNA Technologies, Coralville, IA) containing the assay target sequence. .. The linear range of the ONNV rRT-PCR extended from 8.0 to 2.0 log10 copies/μL ( ).

    Ligation:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

    Synthesized:

    Article Title: Development of a Real-Time Reverse Transcription Polymerase Chain Reaction for O’nyong-nyong Virus and Evaluation with Clinical and Mosquito Specimens from Kenya
    Article Snippet: .. Linear range, precision, and lower limit of 95% detection experiments were performed using synthesized Ultramer Oligos (Integrated DNA Technologies, Coralville, IA) containing the assay target sequence. .. The linear range of the ONNV rRT-PCR extended from 8.0 to 2.0 log10 copies/μL ( ).

    Article Title: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Article Snippet: .. Paired sgRNAs were designed for the ACTB (actin beta, Homo sapiens ; PubMed Gene ID: 60) target gene locus using an online CRISPR gRNA design tool , and synthesized as Ultramer DNA Oligonucleotides (Integrated DNA Technologies), ACTB _sgRNA and ACTB _HDR (Supplementary Table ). .. Next, paired sgRNAs were cloned according to a previously described procedure into a plasmid harboring Cas9D10A and a puromycin resistance cassette .

    Article Title: A 5′ Nuclease Genotyping Assay for Identification of Macrolide-Resistant Mycoplasma genitalium in Clinical Specimens
    Article Snippet: .. As in-run controls, synthesized Ultramer oligonucleotides (Integrated DNA Technologies, Leuven, Belgium) comprising the entire PCR product were used. .. A no-template control was included in each PCR run.

    Construct:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

    Article Title: Synthetic feedback control using an RNAi-based gene-regulatory device
    Article Snippet: .. All miRNA and ribozyme components were constructed by ordering two ultramer oligonucleotides (Integrated DNA Technologies), which were reverse complements of each other at their 3′ ends. .. The oligonucleotide pairs were subjected to five rounds of annealing and extension using Taq polymerase (New England Biolabs) in a thermocycler.

    Purification:

    Article Title: Id genes are essential for early heart formation
    Article Snippet: .. DNA templates for sgRNAs were generated by PCR amplification (Phusion DNA polymerase, New England Biolabs) of ssDNA ultramer oligonucleotides (Integrated DNA Technologies) (see the ); sgRNAs were transcribed from these templates using HiScribe T7 high-yield RNA synthesis kit (New England Biolabs) and purified using Megaclear kit (Life Technologies). .. For mouse zygote injections, 50 ng/µL Cas9 mRNA (Life Technologies) and 20 ng/µL each sgRNA were combined in nuclease-free water.

    Polymerase Chain Reaction:

    Article Title: Id genes are essential for early heart formation
    Article Snippet: .. DNA templates for sgRNAs were generated by PCR amplification (Phusion DNA polymerase, New England Biolabs) of ssDNA ultramer oligonucleotides (Integrated DNA Technologies) (see the ); sgRNAs were transcribed from these templates using HiScribe T7 high-yield RNA synthesis kit (New England Biolabs) and purified using Megaclear kit (Life Technologies). .. For mouse zygote injections, 50 ng/µL Cas9 mRNA (Life Technologies) and 20 ng/µL each sgRNA were combined in nuclease-free water.

    Article Title: A 5′ Nuclease Genotyping Assay for Identification of Macrolide-Resistant Mycoplasma genitalium in Clinical Specimens
    Article Snippet: .. As in-run controls, synthesized Ultramer oligonucleotides (Integrated DNA Technologies, Leuven, Belgium) comprising the entire PCR product were used. .. A no-template control was included in each PCR run.

    Generated:

    Article Title: CRISPR-Cas9–Mediated Modification of the NOD Mouse Genome With Ptpn22R619W Mutation Increases Autoimmune Diabetes
    Article Snippet: .. The HDR template Ptpn22-R619W-HDR1 is a 200-nucleotide-long single-stranded DNA (ssDNA) oligo generated by Ultramer technology (Integrated DNA Technologies). ..

    Article Title: Id genes are essential for early heart formation
    Article Snippet: .. DNA templates for sgRNAs were generated by PCR amplification (Phusion DNA polymerase, New England Biolabs) of ssDNA ultramer oligonucleotides (Integrated DNA Technologies) (see the ); sgRNAs were transcribed from these templates using HiScribe T7 high-yield RNA synthesis kit (New England Biolabs) and purified using Megaclear kit (Life Technologies). .. For mouse zygote injections, 50 ng/µL Cas9 mRNA (Life Technologies) and 20 ng/µL each sgRNA were combined in nuclease-free water.

    CRISPR:

    Article Title: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Article Snippet: .. Paired sgRNAs were designed for the ACTB (actin beta, Homo sapiens ; PubMed Gene ID: 60) target gene locus using an online CRISPR gRNA design tool , and synthesized as Ultramer DNA Oligonucleotides (Integrated DNA Technologies), ACTB _sgRNA and ACTB _HDR (Supplementary Table ). .. Next, paired sgRNAs were cloned according to a previously described procedure into a plasmid harboring Cas9D10A and a puromycin resistance cassette .

    Sequencing:

    Article Title: Development of a Real-Time Reverse Transcription Polymerase Chain Reaction for O’nyong-nyong Virus and Evaluation with Clinical and Mosquito Specimens from Kenya
    Article Snippet: .. Linear range, precision, and lower limit of 95% detection experiments were performed using synthesized Ultramer Oligos (Integrated DNA Technologies, Coralville, IA) containing the assay target sequence. .. The linear range of the ONNV rRT-PCR extended from 8.0 to 2.0 log10 copies/μL ( ).

    Plasmid Preparation:

    Article Title: The Oncogenic Kaposi’s Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family
    Article Snippet: .. 8SK6-5p was based on the previously described lentiviral vector pLCE ( ) and constructed by annealing of 2 pairs of ultramer oligonucleotides (Integrated DNA Technologies) and insertion between XhoI and XbaI sites in the 3′UTR of pLCE by three-fragment ligation. .. For cleavage sensors of miRNA activity (pLCG-3T16, pLCG-3TK6-5p, pLCG-3TK3, pLCG-3TK11; and – ), oligonucleotides were designed and annealed to create sequences containing 3 sites of perfect complementarity to the relevant miRNA and inserted between Xho1 and Xba1 sites of the published lentiviral reporter vector pL/CMV/Fluc (pLCG) ( ).

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  • 96
    Integrated DNA Technologies standard desalted ultramer oligonucleotides
    Standard Desalted Ultramer Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard desalted ultramer oligonucleotides/product/Integrated DNA Technologies
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    standard desalted ultramer oligonucleotides - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

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