Structured Review

Thermo Fisher ssr loci
Ssr Loci, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ssr loci/product/Thermo Fisher
Average 90 stars, based on 27 article reviews
Price from $9.99 to $1999.99
ssr loci - by Bioz Stars, 2020-07
90/100 stars

Images

Related Articles

Polymerase Chain Reaction:

Article Title: Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)
Article Snippet: .. For maximizing the polymorphism, we selected SSR loci with a minimum of 10 repeats for dinucleotides, seven for trinucleotides, and five for tetranucleotides for amplification using a GeneAmp PCR System 9700 thermal cycler (Applied Biosystems, Foster City, USA). .. Reactions were conducted in a total volume of 10 μl containing 2 μl of genomic DNA (50 ng total), 0.25 units Taq DNA polymerase (TaKaRa, Dalian, Liaoning, China), 1 × PCR buffer, 1 μl of 2.5 mM MgCl2 , 1 μl of 2.5 mM dNTPs, 0.1 μl bovine serum albumin (BSA) (TaKaRa, Dalian, Liaoning, China), and 0.5 μl of each 10 μM primer.

Article Title: Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
Article Snippet: .. PCR for amplification of SSR loci were performed using thermal cycler GeneAmp PCR System 9700 (Applied Biosystems). .. The PCR reaction volume was 10 μl containing 5 ng DNA template, 10.0 pmol reverse and forward primers, 1X PCR buffer, 2.0mM of each dNTPs, 1.5mM MgCl2 and 0.3 U of Taq DNA polymerase.

Article Title: A QTL associated with high seed coat cracking rate of a leading Japanese soybean variety
Article Snippet: .. DNA fragments corresponding to the SSR loci were amplified with a GeneAmp PCR 9700 System (Thermo Fisher Scientific, Waltham, MA, USA) using the DNA polymerase reagent AmpliTaq Gold 360 mix (Thermo Fisher Scientific, USA). .. Time and temperature conditions for amplification were as follows: 10 min preheat at 95°C, 35 three-step cycles of 30 sec denaturation at 95°C, 30 sec annealing at 47 °C, and 30 sec extension at 67°C, with a final extension at 67°C for 7 min. PCR products were treated with an automatic electrophoresis machine, LabChip GX Touch (PerkinElmer, Waltham, MA, USA), to check the fragment size.

Multiplex Assay:

Article Title: Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs
Article Snippet: .. For each sample 12 SSR loci were amplified according to a previously published multiplex protocol [ ] that amplified all 12 markers simultaneously and run on a 3730 capillary DNA analyzer according to the manufacturers default settings (Life Technologies, Grand Island, NY). .. The allele peaks were scored in GeneMapper 3.7 (Life Technologies) and exported to a MS Excel spreadsheet.

Amplification:

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: .. The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany). .. Each reaction was performed in 10 μl with the following mixture composition: 20 ng of DNA, 1.5 mM of MgCl2 , 0.4 μM of the first primer, 0.2 μM of the second primer with M13 complementary tail, 0.2 μM of M13 fluorescent labelled primer, and 0.25 U of Taq HotStart DNA polymerase QIAGEN (Valencia, USA).

Article Title: Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)
Article Snippet: .. For maximizing the polymorphism, we selected SSR loci with a minimum of 10 repeats for dinucleotides, seven for trinucleotides, and five for tetranucleotides for amplification using a GeneAmp PCR System 9700 thermal cycler (Applied Biosystems, Foster City, USA). .. Reactions were conducted in a total volume of 10 μl containing 2 μl of genomic DNA (50 ng total), 0.25 units Taq DNA polymerase (TaKaRa, Dalian, Liaoning, China), 1 × PCR buffer, 1 μl of 2.5 mM MgCl2 , 1 μl of 2.5 mM dNTPs, 0.1 μl bovine serum albumin (BSA) (TaKaRa, Dalian, Liaoning, China), and 0.5 μl of each 10 μM primer.

Article Title: Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas
Article Snippet: .. PCR for amplification of SSR loci were performed using thermal cycler GeneAmp PCR System 9700 (Applied Biosystems). .. The PCR reaction volume was 10 μl containing 5 ng DNA template, 10.0 pmol reverse and forward primers, 1X PCR buffer, 2.0mM of each dNTPs, 1.5mM MgCl2 and 0.3 U of Taq DNA polymerase.

Article Title: Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs
Article Snippet: .. For each sample 12 SSR loci were amplified according to a previously published multiplex protocol [ ] that amplified all 12 markers simultaneously and run on a 3730 capillary DNA analyzer according to the manufacturers default settings (Life Technologies, Grand Island, NY). .. The allele peaks were scored in GeneMapper 3.7 (Life Technologies) and exported to a MS Excel spreadsheet.

Article Title: A QTL associated with high seed coat cracking rate of a leading Japanese soybean variety
Article Snippet: .. DNA fragments corresponding to the SSR loci were amplified with a GeneAmp PCR 9700 System (Thermo Fisher Scientific, Waltham, MA, USA) using the DNA polymerase reagent AmpliTaq Gold 360 mix (Thermo Fisher Scientific, USA). .. Time and temperature conditions for amplification were as follows: 10 min preheat at 95°C, 35 three-step cycles of 30 sec denaturation at 95°C, 30 sec annealing at 47 °C, and 30 sec extension at 67°C, with a final extension at 67°C for 7 min. PCR products were treated with an automatic electrophoresis machine, LabChip GX Touch (PerkinElmer, Waltham, MA, USA), to check the fragment size.

Article Title: A set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions
Article Snippet: .. The amplified fragments were assigned as alleles of the appropriate SSR loci using the Genotyper software version 2.5.2 (Applied Biosystems® , Foster City, CA, USA). ..

Software:

Article Title: Identification, utilisation and mapping of novel transcriptome-based markers from blackcurrant (Ribes nigrum)
Article Snippet: .. SSR loci were visualised using ABI PRISM® 3730 Genetic Analyzer and alleles scored using GeneMapper® software (Applied Biosystems Inc., Warrington, UK). ..

Article Title: A set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions
Article Snippet: .. The amplified fragments were assigned as alleles of the appropriate SSR loci using the Genotyper software version 2.5.2 (Applied Biosystems® , Foster City, CA, USA). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher ssr loci
    Ssr Loci, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssr loci/product/Thermo Fisher
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    ssr loci - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results