squalene  (Millipore)


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  • 99
    Name:
    Squalene
    Description:
    Squalene is a hydrocarbon containing double bond commonly found in shark liver oil It is also present in vegetable oils but to a lesser extent
    Catalog Number:
    s3626
    Price:
    None
    Applications:
    Squalene has been used:. as a standard for lipid identification and quantification. in the isolation of macrophages for parasite incubation. as a standard for the quantification of squalene in squalene analysis of oil samples
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    Structured Review

    Millipore squalene
    Squalene
    Squalene is a hydrocarbon containing double bond commonly found in shark liver oil It is also present in vegetable oils but to a lesser extent
    https://www.bioz.com/result/squalene/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    squalene - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Effects of siRNA on RET/PTC3 Junction Oncogene in Papillary Thyroid Carcinoma: From Molecular and Cellular Studies to Preclinical Investigations
    Article Snippet: .. Chemicals Squalene, DMSO (dimethyl sulfoxide), in vitro LDH toxicology assay kit (TOX7), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent, cis -diammine platinum(II) dichloride (cisplatin), puromycin and paraformaldehyde (PFA, 16%) were purchased from Sigma-Aldrich Chemical Co. (Saint Quentin Fallavier, France). .. Dulbecco's modified Eagle medium (DMEM), Opti-MEM, new born (NBCS) and fetal (FCS) calf serums, Lipofectamine 2000 (1 mg/ml), Annexin-V-Fluos with propidium iodide (PI) kit and PCR primers were purchased from Life technologies (Saint Aubin, France).

    Functional Assay:

    Article Title: Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity
    Article Snippet: .. Therefore, two New Zealand White rabbits were immunized intramuscularly with 300 μg VLPs mixed in a 1:1 ratio with squalene-containing water-in-oil Sigma Adjuvant, which was recently demonstrated to enhance the induction of functional antibody titers against Ebola virus and Nipah virus . ..

    In Vitro:

    Article Title: Effects of siRNA on RET/PTC3 Junction Oncogene in Papillary Thyroid Carcinoma: From Molecular and Cellular Studies to Preclinical Investigations
    Article Snippet: .. Chemicals Squalene, DMSO (dimethyl sulfoxide), in vitro LDH toxicology assay kit (TOX7), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent, cis -diammine platinum(II) dichloride (cisplatin), puromycin and paraformaldehyde (PFA, 16%) were purchased from Sigma-Aldrich Chemical Co. (Saint Quentin Fallavier, France). .. Dulbecco's modified Eagle medium (DMEM), Opti-MEM, new born (NBCS) and fetal (FCS) calf serums, Lipofectamine 2000 (1 mg/ml), Annexin-V-Fluos with propidium iodide (PI) kit and PCR primers were purchased from Life technologies (Saint Aubin, France).

    Infection:

    Article Title: An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway
    Article Snippet: .. Metabolite and LXR Agonist Treatment of Cells After washing, normal medium containing vehicle (Ethanol) or GGOH (Sigma G3278), Mevalonolactone (MEV) (Sigma M4667), FOH (Sigma F203), or SQL (Sigma S3626) was added to the infected wells. .. T0901317 (Tocris Bioscience, Bristol, UK) was resuspended in 100% ethanol (5 mM stock), diluted in normal medium (50 nM final concentration) and cells were treated for 18 h.

    Article Title: Developmentally Regulated Sesquiterpene Production Confers Resistance to Colletotrichum gloeosporioides in Ripe Pepper Fruits
    Article Snippet: .. To examine the effect of the inhibitor of squalene synthase on the infected fruits, 10 µM zaragozic acid (ZA; Sigma) was applied directly to the surface of the fruits for 3 h at 28°C before inoculation with fungal spores separately next to the ZA drop on the fruits. .. Antifungal activity of capsidiol Capsidiol was extracted from pepper fruits treated with 0.1% cellulase for 2 days, and purified as described previously .

    Mouse Assay:

    Article Title: Generation of therapeutic antisera for emerging viral infections
    Article Snippet: .. To generate mouse antisera, groups of 6-week-old Balb/c mice were immunized intramuscularly with either 2 × 105 PFU of VSVΔG/EBOV-GP, 1.5 × 108 PFU of either MVA/EBOV-VP40/GP or MVA/EBOV-GP, or 10 µg of EBOV VLPs mixed in a 1:1 ratio (v/v) with either Sigma Adjuvant System (0.5 mg monophosphoryl lipid A (detoxified endotoxin) from Salmonella minnesota and 0.5 mg synthetic trehalose dicorynomycolate in 2% squalene-Tween-80-water, Sigma), or TiterMax Gold (block polymer CRL-8300, squalene, and sorbitan monooleate, Sigma), or 2% Alhydrogel (Invivogen). .. Three mice from each immunization approach were sacrificed at 0, 14, 28, and 42 days post-immunization and blood was collected by cardiac puncture.

    Article Title: Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an Inflammatory Response
    Article Snippet: .. Squalene treatment Foxp3 -GFP+/− , TCR-HA+/− double transgenic (dTg) mice (F1 hybrids) were injected intraperitoneally (i.p.) with one or four doses of 180 µg squalene (Sigma, Atlanta, GA, USA) emulsified 3∶1 in saline. .. At various time-points after the last injection, the blood, liver and spleen were collected for histology, in vitro bioassays, serum lipid electrophoresis, FACS analysis, and adoptive cell transfer experiments.

    Transgenic Assay:

    Article Title: Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an Inflammatory Response
    Article Snippet: .. Squalene treatment Foxp3 -GFP+/− , TCR-HA+/− double transgenic (dTg) mice (F1 hybrids) were injected intraperitoneally (i.p.) with one or four doses of 180 µg squalene (Sigma, Atlanta, GA, USA) emulsified 3∶1 in saline. .. At various time-points after the last injection, the blood, liver and spleen were collected for histology, in vitro bioassays, serum lipid electrophoresis, FACS analysis, and adoptive cell transfer experiments.

    other:

    Article Title: Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans
    Article Snippet: Chemicals and reagents Squalene, cholesterol, β-sitosterol, lanosterol, cycloartenol, campesterol, lathosterol, desmosterol, fucosterol, phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, lysophosphatidylethanolamine, lysophosphatidylcholine and ceramide phosphorylethanolamine were purchased from Sigma Aldrich (St Louis, MO, USA).

    Blocking Assay:

    Article Title: Generation of therapeutic antisera for emerging viral infections
    Article Snippet: .. To generate mouse antisera, groups of 6-week-old Balb/c mice were immunized intramuscularly with either 2 × 105 PFU of VSVΔG/EBOV-GP, 1.5 × 108 PFU of either MVA/EBOV-VP40/GP or MVA/EBOV-GP, or 10 µg of EBOV VLPs mixed in a 1:1 ratio (v/v) with either Sigma Adjuvant System (0.5 mg monophosphoryl lipid A (detoxified endotoxin) from Salmonella minnesota and 0.5 mg synthetic trehalose dicorynomycolate in 2% squalene-Tween-80-water, Sigma), or TiterMax Gold (block polymer CRL-8300, squalene, and sorbitan monooleate, Sigma), or 2% Alhydrogel (Invivogen). .. Three mice from each immunization approach were sacrificed at 0, 14, 28, and 42 days post-immunization and blood was collected by cardiac puncture.

    Injection:

    Article Title: Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an Inflammatory Response
    Article Snippet: .. Squalene treatment Foxp3 -GFP+/− , TCR-HA+/− double transgenic (dTg) mice (F1 hybrids) were injected intraperitoneally (i.p.) with one or four doses of 180 µg squalene (Sigma, Atlanta, GA, USA) emulsified 3∶1 in saline. .. At various time-points after the last injection, the blood, liver and spleen were collected for histology, in vitro bioassays, serum lipid electrophoresis, FACS analysis, and adoptive cell transfer experiments.

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  • 99
    Millipore squalene inhibitor
    Squalene Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/squalene inhibitor/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    squalene inhibitor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Millipore squalens cbhi
    Gel electrophoretic (SDS-PAGE) separation and isoelectric focusing (IEF) of D . <t>squalens</t> enzymes. (A) SDS-PAGE of chromatographically purified <t>CBHI</t> fraction, heterologously produced rCel6A, and purified CDH of D . squalens . Lane 1, CBHI fraction; lane 2, rCel6A; lane 3, T . reesei culture filtrate without cel6a insert; lane 4 CDH. (B) IEF analysis of CBHI fraction of D . squalens .
    Squalens Cbhi, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/squalens cbhi/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    squalens cbhi - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Gel electrophoretic (SDS-PAGE) separation and isoelectric focusing (IEF) of D . squalens enzymes. (A) SDS-PAGE of chromatographically purified CBHI fraction, heterologously produced rCel6A, and purified CDH of D . squalens . Lane 1, CBHI fraction; lane 2, rCel6A; lane 3, T . reesei culture filtrate without cel6a insert; lane 4 CDH. (B) IEF analysis of CBHI fraction of D . squalens .

    Journal: PLoS ONE

    Article Title: Saccharification of Lignocelluloses by Carbohydrate Active Enzymes of the White Rot Fungus Dichomitus squalens

    doi: 10.1371/journal.pone.0145166

    Figure Lengend Snippet: Gel electrophoretic (SDS-PAGE) separation and isoelectric focusing (IEF) of D . squalens enzymes. (A) SDS-PAGE of chromatographically purified CBHI fraction, heterologously produced rCel6A, and purified CDH of D . squalens . Lane 1, CBHI fraction; lane 2, rCel6A; lane 3, T . reesei culture filtrate without cel6a insert; lane 4 CDH. (B) IEF analysis of CBHI fraction of D . squalens .

    Article Snippet: Purification and characterization of native D . squalens CBHI and CDH enzymes The frozen culture liquid was melted, filtered through Miracloth (Calbiochem, USA) and Whatman GF/C glass microfibre filters (England) and concentrated by using Filtron Minisette apparatus (with 10.000 NMWL filter casettes) and Amicon ultrafiltration unit with 10.000 NMWL polyethersulphone membranes (Sartorius, Germany) at 4°C.

    Techniques: SDS Page, Electrofocusing, Purification, Produced

    Characterization of CBHI fraction of D . squalens ( A) Temperature (10 min reaction, ■; 1 h reaction, ━) and (B) pH optimum, (C) thermostability at 40 (■), 50 (○), 60 (▲) and 70°C (◇), and (D) pH stability at pH 3.0 (■), pH 4.0 (●), pH 5.0 (△), pH 6.0 (◆), pH 6.5 (□) and in distilled water (○) of the purified CBHI fraction. Reactions were conducted in 50 mM sodium citrate buffer at pH 5.0 with MULac as substrate, except pH stability, which was studied in various buffer pH values and in distilled water. Standard deviations of the activities of three technical replicates are shown as error bars.

    Journal: PLoS ONE

    Article Title: Saccharification of Lignocelluloses by Carbohydrate Active Enzymes of the White Rot Fungus Dichomitus squalens

    doi: 10.1371/journal.pone.0145166

    Figure Lengend Snippet: Characterization of CBHI fraction of D . squalens ( A) Temperature (10 min reaction, ■; 1 h reaction, ━) and (B) pH optimum, (C) thermostability at 40 (■), 50 (○), 60 (▲) and 70°C (◇), and (D) pH stability at pH 3.0 (■), pH 4.0 (●), pH 5.0 (△), pH 6.0 (◆), pH 6.5 (□) and in distilled water (○) of the purified CBHI fraction. Reactions were conducted in 50 mM sodium citrate buffer at pH 5.0 with MULac as substrate, except pH stability, which was studied in various buffer pH values and in distilled water. Standard deviations of the activities of three technical replicates are shown as error bars.

    Article Snippet: Purification and characterization of native D . squalens CBHI and CDH enzymes The frozen culture liquid was melted, filtered through Miracloth (Calbiochem, USA) and Whatman GF/C glass microfibre filters (England) and concentrated by using Filtron Minisette apparatus (with 10.000 NMWL filter casettes) and Amicon ultrafiltration unit with 10.000 NMWL polyethersulphone membranes (Sartorius, Germany) at 4°C.

    Techniques: Purification