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The performance of <t>ONT</t> rapid <t>barcoding</t> method mediated NGS with library preparation from samples with sufficient amplicons. (A) Histograms of the Ct value distribution for clinical samples having sufficient (dark green) and insufficient (dark red) amplicons generated for ONT libraries. (B) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group with RSV full‐length genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’), respectively. (C) Coverage depth of sequenced representative RSV‐A (red) and RSV‐B (blue) in genomic position covered by six overlapping amplicons. (D) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group having RSV whole genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’ in dark green), whole genome rescued by DNA purification (purple), respectively. Coverage depth of sequenced representative RSV‐A (E) and RSV‐B (F) in genomic position with (blue) and without (red) PCR amplicon clean‐up. Bar plots showing the fold change of NGS reads mapped to RSV reference genomes from sequenced samples of low (G) and high (H) concentration of amplicons generated without and with PCR amplicon purification. NGS reads of libraries from washed PCR amplicons were normalised and expressed as fold changes to ones from unwashed PCR amplicons that were set to 1. Data are expressed as mean ± SD. t‐test analysis was performed for statistical significance. P values less than 0.05 were considered as statistically significant and labelled as * in the figures.
Ont Rapid Barcoding Kit Sqk Rbk114.96, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sqk-rbk114%2E96/pmc12037990-65-6-10?v=Oxford+Nanopore
Average 90 stars, based on 1 article reviews
ont rapid barcoding kit sqk-rbk114.96 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
rapid barcoding kit sqk-rbk114.96  (Oxford Nanopore)
90
Oxford Nanopore rapid barcoding kit sqk-rbk114.96
The performance of <t>ONT</t> rapid <t>barcoding</t> method mediated NGS with library preparation from samples with sufficient amplicons. (A) Histograms of the Ct value distribution for clinical samples having sufficient (dark green) and insufficient (dark red) amplicons generated for ONT libraries. (B) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group with RSV full‐length genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’), respectively. (C) Coverage depth of sequenced representative RSV‐A (red) and RSV‐B (blue) in genomic position covered by six overlapping amplicons. (D) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group having RSV whole genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’ in dark green), whole genome rescued by DNA purification (purple), respectively. Coverage depth of sequenced representative RSV‐A (E) and RSV‐B (F) in genomic position with (blue) and without (red) PCR amplicon clean‐up. Bar plots showing the fold change of NGS reads mapped to RSV reference genomes from sequenced samples of low (G) and high (H) concentration of amplicons generated without and with PCR amplicon purification. NGS reads of libraries from washed PCR amplicons were normalised and expressed as fold changes to ones from unwashed PCR amplicons that were set to 1. Data are expressed as mean ± SD. t‐test analysis was performed for statistical significance. P values less than 0.05 were considered as statistically significant and labelled as * in the figures.
Rapid Barcoding Kit Sqk Rbk114.96, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sqk-rbk114%2E96/pm40138716-112-9-13?v=Oxford+Nanopore
Average 90 stars, based on 1 article reviews
rapid barcoding kit sqk-rbk114.96 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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The performance of ONT rapid barcoding method mediated NGS with library preparation from samples with sufficient amplicons. (A) Histograms of the Ct value distribution for clinical samples having sufficient (dark green) and insufficient (dark red) amplicons generated for ONT libraries. (B) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group with RSV full‐length genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’), respectively. (C) Coverage depth of sequenced representative RSV‐A (red) and RSV‐B (blue) in genomic position covered by six overlapping amplicons. (D) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group having RSV whole genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’ in dark green), whole genome rescued by DNA purification (purple), respectively. Coverage depth of sequenced representative RSV‐A (E) and RSV‐B (F) in genomic position with (blue) and without (red) PCR amplicon clean‐up. Bar plots showing the fold change of NGS reads mapped to RSV reference genomes from sequenced samples of low (G) and high (H) concentration of amplicons generated without and with PCR amplicon purification. NGS reads of libraries from washed PCR amplicons were normalised and expressed as fold changes to ones from unwashed PCR amplicons that were set to 1. Data are expressed as mean ± SD. t‐test analysis was performed for statistical significance. P values less than 0.05 were considered as statistically significant and labelled as * in the figures.

Journal: Influenza and Other Respiratory Viruses

Article Title: An Improved Rapid and Sensitive Long Amplicon Method for Nanopore‐Based RSV Whole‐Genome Sequencing

doi: 10.1111/irv.70106

Figure Lengend Snippet: The performance of ONT rapid barcoding method mediated NGS with library preparation from samples with sufficient amplicons. (A) Histograms of the Ct value distribution for clinical samples having sufficient (dark green) and insufficient (dark red) amplicons generated for ONT libraries. (B) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group with RSV full‐length genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’), respectively. (C) Coverage depth of sequenced representative RSV‐A (red) and RSV‐B (blue) in genomic position covered by six overlapping amplicons. (D) Histograms of the Ct value distribution for clinical samples within sufficient amplicons group having RSV whole genome (blue), G plus F genes (brown), only G gene (dark red) and missing G and F gene sequenced (also called ‘other’ in dark green), whole genome rescued by DNA purification (purple), respectively. Coverage depth of sequenced representative RSV‐A (E) and RSV‐B (F) in genomic position with (blue) and without (red) PCR amplicon clean‐up. Bar plots showing the fold change of NGS reads mapped to RSV reference genomes from sequenced samples of low (G) and high (H) concentration of amplicons generated without and with PCR amplicon purification. NGS reads of libraries from washed PCR amplicons were normalised and expressed as fold changes to ones from unwashed PCR amplicons that were set to 1. Data are expressed as mean ± SD. t‐test analysis was performed for statistical significance. P values less than 0.05 were considered as statistically significant and labelled as * in the figures.

Article Snippet: ONT NGS libraries were prepared with ONT Rapid Barcoding Kit (Oxford Nanopore Technologies, SQK‐RBK114.96) or Rapid PCR Barcoding Kit (Oxford Nanopore Technologies, SQK‐RPB114.24) according to the manufacturer's instructions.

Techniques: Generated, DNA Purification, Amplification, Concentration Assay, Purification