spry1 sirna (mouse; santa cruz biotechnology, sc-41036)  (Santa Cruz Biotechnology)

Journal: IBRO Neuroscience Reports

Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma

doi: 10.1016/j.ibneur.2022.07.003

Figure Lengend Snippet: SPRY1 is highly expressed in GBM, A. SPRY1 expression in GBM was compared using RNA-seq of REMBRANDT dataset. Data are mean ± SEM (nontumor, n = 28; astrocytoma, n = 147; oligodendroglioma, n = 67; GBM, n = 219). * **P < .001. B. SPRY1 expression compared GBM grades (Grade I and II, n = 100; Grade III, n = 85; Grade IV, n = 130). * **P < .001. C. Using microarray of REMBRANDT dataset, Kaplan-Meier survival curves of 397 GBM patients (SPRY1 high n = 185, SPRY1 low n = 212, P < .0001). D. SPRY1 expression compared IDH-mutant GBM.

Article Snippet: Small interfering RNAs (siRNA) for inhibiting the SPRY1 transcript, were purchased from Bioneer (Daejeon, South Korea).

Techniques: Expressing, RNA Sequencing, Microarray, Mutagenesis

Journal: IBRO Neuroscience Reports

Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma

doi: 10.1016/j.ibneur.2022.07.003

Figure Lengend Snippet: SPRY1 is highly expressed in glioma cells and glioma stem cells than in normal astrocytes. A. Real-time qPCR analysis of SPRY1 mRNA expression in normal human astrocyte (NHA), glioma cells, and glioma stem cells. NHA vs. glioma cells or glioma stem cells. B and C. mRNA expression of SPRY1 , stem cell markers, and differentiation markers in NBE cultured and FBS cultured GSC11 (B) and GSC23(C). NBE vs. FBS in GSC11 and GSC23. Data are means ± SEM (n = 3). Data are means ± SEM (n = 3). *P < .05, * **P < .001, * ** *P < .0001.

Article Snippet: Small interfering RNAs (siRNA) for inhibiting the SPRY1 transcript, were purchased from Bioneer (Daejeon, South Korea).

Techniques: Expressing, Cell Culture

Journal: IBRO Neuroscience Reports

Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma

doi: 10.1016/j.ibneur.2022.07.003

Figure Lengend Snippet: Knockdown of SPRY1 inhibits the stemness of GSCs, A . Realtime-qPCR indicated SPRY1 knockdown efficiency using siRNA transfection in GSCs. B. Measurement of cell proliferation rate in NT (Non-targeting siRNA) and siSPRY1. *P < .05, * *P < .01. C. Effect of SPRY1 knockdown on neurosphere formation in GSC11. Data are means ± SEM (n = 3). * *P < .01. Scale bars represent 100 µm. D. Effect of SPRY1 knockdown on stem cell markers, CD15 and CD133 mRNA expression in GSC11. Data are means ± SEM (n = 3). *P < .05, * ** *P < .0001.

Article Snippet: Small interfering RNAs (siRNA) for inhibiting the SPRY1 transcript, were purchased from Bioneer (Daejeon, South Korea).

Techniques: Knockdown, Transfection, Expressing

Journal: IBRO Neuroscience Reports

Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma

doi: 10.1016/j.ibneur.2022.07.003

Figure Lengend Snippet: SPRY1 positively correlates with genes associated with cancer stem cell stemness pathway. A. Upregulated genes correlated with high expression of SPRY1 analysis to Gene Ontology analysis (GO analysis) in REMBRANDT dataset. B. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in REMBRANDT microarray dataset. C. Gene Set Enrichment Analysis (GSEA) in REMBRANDT; Representative enriched pathways in SPRY1 positive correlated samples.

Article Snippet: Small interfering RNAs (siRNA) for inhibiting the SPRY1 transcript, were purchased from Bioneer (Daejeon, South Korea).

Techniques: Expressing, Microarray

Journal: IBRO Neuroscience Reports

Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma

doi: 10.1016/j.ibneur.2022.07.003

Figure Lengend Snippet: SPRY1 correlated GBM subtype and region. A. Comparison of SPRY1 mRNA levels among the groups with GBM subtypes (Classical n = 99, Neural n = 39, Mesenchymal n = 37, Proneural n = 44) * ** P < .001. B. The graph of SPRY1 between GBM subtypes markers correlation in REMRANDT dataset. C. GSEA analysis of REMBRANDT dataset, SPRY1 positive samples correlated with GBM subtypes. D. Heatmap of SPRY1 expression signature correlated with GBM region in the Ivy GAP RNAseq dataset. CT, cellular tumor; IT, infiltration tumor; LE, leading edge; MVP, microvascular proliferation; HBV, hyperplastic blood vessels; PAN, pseudopalisading cells around necrosis; PNZ, perinecrotic zone.

Article Snippet: Small interfering RNAs (siRNA) for inhibiting the SPRY1 transcript, were purchased from Bioneer (Daejeon, South Korea).

Techniques: Comparison, Expressing

Journal: Journal of cellular biochemistry

Article Title: Correlation of Sprouty1 and Jagged1 With Aggressive Prostate Cancer Cells With Different Sensitivities to Androgen Deprivation

doi: 10.1002/jcb.24805

Figure Lengend Snippet: Cell shapes and expression levels of SPRY1 and JAG1 in LNCaP-P, -cl1, and -cl5. A: The cell shapes of LNCaP-P, -cl1, and -cl5 (bars: 50 μm), B: SPRY1 and JAG1 mRNA expression levels relative to TBP by real-time PCR. C: SPRY1 and JAG1 protein expression levels by Western blotting.

Article Snippet: RNA INTERFERENCE Cells were plated at 2–3 × 10 5 per well in six well plates and transfected after incubation for 24 h. ON-TARGET plus SMART pool siRNA for SPRY1 (J27339) JAG1 (J011060) and negative control siRNA (D1810), miRIDIAN Mimic hsa-miR-21 (C300492) and miRIDIAN Mimic negative control (CN1000), lentiviral shRNA vector pGIPZ-shJAG1 (V2LHS-255871) were all purchased from Thermo Scientific.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of cellular biochemistry

Article Title: Correlation of Sprouty1 and Jagged1 With Aggressive Prostate Cancer Cells With Different Sensitivities to Androgen Deprivation

doi: 10.1002/jcb.24805

Figure Lengend Snippet: Regulation of SPRY1 and JAG1 by miR-21. A: miR-21 expression levels relative to RNU24 in LNCaP-cl1 and -cl5 by real-time PCR. B: miR-21, SPRY1, and JAG1 mRNA expression changes under the stimulation with 0, 0.1, 1, and 10 nM of synthetic androgen (R1881). C: SPRY1 and JAG1 mRNA (upper) and SPRY1 and JAG1 protein (lower) expression levels of LNCaP cells transfected with a negative control microRNA (miR-Ctr) and hsa-miR-21 (miR-21). (*P < 0.05, **P < 0.005).

Article Snippet: RNA INTERFERENCE Cells were plated at 2–3 × 10 5 per well in six well plates and transfected after incubation for 24 h. ON-TARGET plus SMART pool siRNA for SPRY1 (J27339) JAG1 (J011060) and negative control siRNA (D1810), miRIDIAN Mimic hsa-miR-21 (C300492) and miRIDIAN Mimic negative control (CN1000), lentiviral shRNA vector pGIPZ-shJAG1 (V2LHS-255871) were all purchased from Thermo Scientific.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Negative Control

Journal: Journal of cellular biochemistry

Article Title: Correlation of Sprouty1 and Jagged1 With Aggressive Prostate Cancer Cells With Different Sensitivities to Androgen Deprivation

doi: 10.1002/jcb.24805

Figure Lengend Snippet: SPRY1 suppresses the EGF/ERK/AR pathway. A: SPRY1 protein expression levels by Western blotting in LNCaP cells transfected with a negative control (siCtr) and SPRY1 targeting (siSPRY1) siRNA. B: AR activation of LNCaP-siCtr and -siSPRY1 evaluated by dual luciferase reporter assay using pGL3-luc containing PSA promoter (PSAp) and pTK-RL (TK). C: PSA mRNA expression levels relative to TBP (upper) and cell proliferation in MTS assays (lower) of LNCaP-siCtr and -siSPRY1 cells in normal medium (FBS), androgen-depleted medium without EGF (CSFBS), and androgen-depleted medium with 2 ng/ml EGF (CSFBS + EGF). D: PSA mRNA expression levels relative to TBP (upper) and cell proliferation in MTS assays (lower) of LNCaP-siCtr and -siSPRY1 cells in normal medium with 0, 5, and 10 μM of ERK inhibitor PD98059 (PD). Cells were incubated for 72 h. (*P < 0.05, **P < 0.005). E: AR regulates miR-21. miR-21 negatively regulate SPRY1. SPRY1 negatively regulate the EGF/ERK/AR pathway.

Article Snippet: RNA INTERFERENCE Cells were plated at 2–3 × 10 5 per well in six well plates and transfected after incubation for 24 h. ON-TARGET plus SMART pool siRNA for SPRY1 (J27339) JAG1 (J011060) and negative control siRNA (D1810), miRIDIAN Mimic hsa-miR-21 (C300492) and miRIDIAN Mimic negative control (CN1000), lentiviral shRNA vector pGIPZ-shJAG1 (V2LHS-255871) were all purchased from Thermo Scientific.

Techniques: Expressing, Western Blot, Transfection, Negative Control, Activation Assay, Luciferase, Reporter Assay, Incubation

Journal: Journal of cellular biochemistry

Article Title: Correlation of Sprouty1 and Jagged1 With Aggressive Prostate Cancer Cells With Different Sensitivities to Androgen Deprivation

doi: 10.1002/jcb.24805

Figure Lengend Snippet: JAG1 enhances cell invasiveness. A: JAG1 protein expression levels of LNCaP cells transfected with negative control (siCtr) and JAG1 targeting (siJAG1) siRNAs by Western blotting (left). Their cell proliferation in MTS assays after 72 h incubation (right). B: Cell proliferation of LNCaP cells under the administration of 0, 5, and 10 μg/ml JAG1 recombinant protein (left). Numbers of invaded LNCaP cells without or with 10 μg/ml JAG1 recombinant protein in Matrigel invasion assays after 72 h incubation (right). C: JAG1 protein expression levels of PC3 cells transfected with pGIPZ Non-silencing control (shCtr) and pGIPZ-shJAG1 (shJAG1) by Western blotting (left). Numbers of invaded PC3-shCtr and -shJAG1 cells in Matrigel invasion assays after 48 h incubation (right). D: Sequential changes in tumor volumes of PC3-shCtr and -shJAG1 xenograft tumor at indicated days after transplantation (n = 5 each). (*P < 0.05, **P < 0.005).

Article Snippet: RNA INTERFERENCE Cells were plated at 2–3 × 10 5 per well in six well plates and transfected after incubation for 24 h. ON-TARGET plus SMART pool siRNA for SPRY1 (J27339) JAG1 (J011060) and negative control siRNA (D1810), miRIDIAN Mimic hsa-miR-21 (C300492) and miRIDIAN Mimic negative control (CN1000), lentiviral shRNA vector pGIPZ-shJAG1 (V2LHS-255871) were all purchased from Thermo Scientific.

Techniques: Expressing, Transfection, Negative Control, Western Blot, Incubation, Recombinant, Control, Transplantation Assay

Journal: Journal of cellular biochemistry

Article Title: Correlation of Sprouty1 and Jagged1 With Aggressive Prostate Cancer Cells With Different Sensitivities to Androgen Deprivation

doi: 10.1002/jcb.24805

Figure Lengend Snippet: SPRY1 and JAG1 mRNA expression levels in PC tissues. A: SPRY1 mRNA expression levels by real time PCR in prostate cancer tissues of patients without (–) and with (+) PSA recurrence. B:JAG1 mRNA expression levels by real time PCR in prostate cancer tissues with Gleason sum score (GS) 5–6, 7, and 8–9 diseases. C:JAG1 mRNA expression levels by real time PCR in PC tissues with Gleason sum score (GS) 5–6, 7, and 8–9 diseases. (*P < 0.05, **P < 0.005).

Article Snippet: RNA INTERFERENCE Cells were plated at 2–3 × 10 5 per well in six well plates and transfected after incubation for 24 h. ON-TARGET plus SMART pool siRNA for SPRY1 (J27339) JAG1 (J011060) and negative control siRNA (D1810), miRIDIAN Mimic hsa-miR-21 (C300492) and miRIDIAN Mimic negative control (CN1000), lentiviral shRNA vector pGIPZ-shJAG1 (V2LHS-255871) were all purchased from Thermo Scientific.

Techniques: Expressing, Real-time Polymerase Chain Reaction