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Ficoll-Paque Pharmacia spleen mononuclear cells mncs
Spleen Mononuclear Cells Mncs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spleen mononuclear cells mncs/product/Ficoll-Paque Pharmacia
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
spleen mononuclear cells mncs - by Bioz Stars, 2021-03
86/100 stars

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Isolation:

Article Title: Invariant NKT Cell Lines Derived from the NOD?H2h4 Mouse Enhance Autoimmune Thyroiditis
Article Snippet: Cell Preparation and Development of Cell Lines NOD·H2h4 mice were fed a low dose of sodium iodide (0.05%) in drinking water for 12 weeks. .. Spleen mononuclear cells (MNCs) were isolated by density gradient centrifugation on Ficoll Paque (Pharmacia, Biotech, Sweden). .. Cells were washed and cultured in 24 well plates at a density of 106 cells/mL with complete RPMI 1640 supplemented with 10% FBS, 20 IU/mL penicillin, 20 μ g/mL streptomycin, 20 mM/L L-glutamine, and 100 μ M nonessential amino acids (all from GIBCO BRL, MD, USA).

Article Title: The NAD-dependent deacetylase SIRT2 regulates T cell differentiation involved in tumor immune response
Article Snippet: .. Flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from blood samples through the Ficoll-Paque Plus (17144002, GE) density gradient separation method. .. The mice spleens were obtained from wild type and Sirt2 -/- mice removing the red blood cells by red blood cell lysis buffer (R1010, Solarbio).

Article Title: MOSPD2 is a therapeutic target for the treatment of CNS inflammation
Article Snippet: Isolation of mouse and human immune cell subsetsMouse monocytes and T cells were isolated from the spleen using CD11b (cat. no. 130‐049‐601) and CD90.2 (cat. no. 130‐049‐101) microbeads, respectively. .. Peripheral blood mononuclear cells (PBMCs) were isolated on Ficoll‐Paque Plus (cat. no. 17‐1440‐03; GE Healthcare, Chicago, IL, USA) using 50‐ml Leucosep tubes (cat. no. 227290; Greiner Bio‐One, Kremsmünster, Austria). .. Cells were washed in phosphate‐buffered saline (PBS) (Biological Industries, Beit HaEmek, Israel), and incubated at 4°C for 15 min in buffer containing PBS and 0·5% bovine serum albumin (BSA) with human CD14 microbeads (cat. no. 130‐050‐201; Miltenyi Biotec, Bergisch Gladbach, Germany).

Article Title: The Antitumor Effect of Heparin is not Mediated by Direct NK Cell Activation
Article Snippet: NK Cell Isolation and Culture NK cells from spleens of WT and Ncr1 −/− mice were isolated by organ maceration, followed by selection using a mouse NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. For human NK cell isolation, peripheral blood mononuclear cells (PBMCs) were first isolated from fresh umbilical cord blood by Ficoll-Paque density (1.077 g/mL) centrifugation (Sigma-Aldrich, St. Louis, MO, USA). .. NK cells were enriched from resulting PBMCs by following negative selection using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies, Vancouver, BC, Canada).

Gradient Centrifugation:

Article Title: Invariant NKT Cell Lines Derived from the NOD?H2h4 Mouse Enhance Autoimmune Thyroiditis
Article Snippet: Cell Preparation and Development of Cell Lines NOD·H2h4 mice were fed a low dose of sodium iodide (0.05%) in drinking water for 12 weeks. .. Spleen mononuclear cells (MNCs) were isolated by density gradient centrifugation on Ficoll Paque (Pharmacia, Biotech, Sweden). .. Cells were washed and cultured in 24 well plates at a density of 106 cells/mL with complete RPMI 1640 supplemented with 10% FBS, 20 IU/mL penicillin, 20 μ g/mL streptomycin, 20 mM/L L-glutamine, and 100 μ M nonessential amino acids (all from GIBCO BRL, MD, USA).

Flow Cytometry:

Article Title: The NAD-dependent deacetylase SIRT2 regulates T cell differentiation involved in tumor immune response
Article Snippet: .. Flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from blood samples through the Ficoll-Paque Plus (17144002, GE) density gradient separation method. .. The mice spleens were obtained from wild type and Sirt2 -/- mice removing the red blood cells by red blood cell lysis buffer (R1010, Solarbio).

Cell Isolation:

Article Title: The Antitumor Effect of Heparin is not Mediated by Direct NK Cell Activation
Article Snippet: NK Cell Isolation and Culture NK cells from spleens of WT and Ncr1 −/− mice were isolated by organ maceration, followed by selection using a mouse NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. For human NK cell isolation, peripheral blood mononuclear cells (PBMCs) were first isolated from fresh umbilical cord blood by Ficoll-Paque density (1.077 g/mL) centrifugation (Sigma-Aldrich, St. Louis, MO, USA). .. NK cells were enriched from resulting PBMCs by following negative selection using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies, Vancouver, BC, Canada).

Centrifugation:

Article Title: The Antitumor Effect of Heparin is not Mediated by Direct NK Cell Activation
Article Snippet: NK Cell Isolation and Culture NK cells from spleens of WT and Ncr1 −/− mice were isolated by organ maceration, followed by selection using a mouse NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. For human NK cell isolation, peripheral blood mononuclear cells (PBMCs) were first isolated from fresh umbilical cord blood by Ficoll-Paque density (1.077 g/mL) centrifugation (Sigma-Aldrich, St. Louis, MO, USA). .. NK cells were enriched from resulting PBMCs by following negative selection using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies, Vancouver, BC, Canada).

Purification:

Article Title: Sequencing B cell receptors from ferrets (Mustela putorius furo)
Article Snippet: .. PBMCs were purified using 95% Ficoll-Paque Plus and cryopreserved in heat-inactivated fetal calf serum (FCS) containing 10% dimethylsulfoxide (DMSO). .. Cryopreserved ferret PBMCs were thawed, stained with Live/Dead Fixable Aqua (Thermo Fisher), surface stains anti-CD11b-BV510 (Biolegend: clone M1/70), anti-CD8-BV450 (Thermo Fisher: clone OKT8) and anti-ferret IgA/IgM/IgG-FITC (Rockland Immunochemicals cat.618-102-130).

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    Ficoll-Paque Pharmacia spleen mononuclear cells mncs
    Spleen Mononuclear Cells Mncs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spleen mononuclear cells mncs/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spleen mononuclear cells mncs - by Bioz Stars, 2021-03
    86/100 stars
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