spin filters (Valiant)

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Bioz Stars

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Name:

Spin Filters

Description:

Catalog Number:

116560210

Price:

75.0

Category:

Life Sciences Sample Preparation DNA RNA Isolation and Purification Genomic DNA Isolation

Size:

50 each

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Structured Review

Valiant spin filters

https://www.bioz.com/result/spin filters/product/Valiant
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

spin filters - by Bioz Stars, 2021-04

93/100 stars

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Article Title: Cleaved amplified polymorphic sequences (CAPS) marker for identification of two mutant alleles of the rapeseed BnaA.FAD2 gene
Article Snippet: The suspension on the SPIN Filter was then washed with 500 μl of SEWS-M Wash Solution with ethanol (a mixture of 12 ml of Concentrated SEWS-M Wash Solution and 100 ml of 96% ethanol) (MP Biomedicals) and centrifuged two times.

Binding Assay:

Article Title: Metagenomics Strain Resolution on Assembly Graphs
Article Snippet: Precipitated proteins were pelleted for five minutes at 14000 g and the supernatant was transferred to 1000 µL of pre mixed DNA binding matrix solution (cat no: 116540408). .. DNA binding matrix beads were recovered using the MP bio™ spin filter (cat no: 116560210) and manufacturer based spin protocol. .. The binding matrix was washed of impurities by complete resuspension in 500 µL of SEWS-M solution (cat no: 116540405) and centrifuged at 14000 g for five minutes.

Centrifugation:

Article Title: Quantitative PCR Monitoring of Antibiotic Resistance Genes and Bacterial Pathogens in Three European Artificial Groundwater Recharge Systems ▿Quantitative PCR Monitoring of Antibiotic Resistance Genes and Bacterial Pathogens in Three European Artificial Groundwater Recharge Systems ▿ †
Article Snippet: DNA was extracted from bacterial cultures and wastewater samples with a FastDNA spin kit for soil (MP Biomedicals Europe, Heidelberg, Germany) according to the manufacturer's instruction, with minor modifications. .. The spin filter centrifugation step with 500 μl salt-ethanol wash solution (SEWS-M buffer; MP Biomedicals Europe, Heidelberg, Germany) was performed at 14,000 × g for 2 min. DNA was eluted with 100 μl distilled water. .. The concentration and integrity of extracted DNA were verified by electrophoresis on 0.7% agarose gels.

Isolation:

Article Title: Efficacy and gut dysbiosis of gentamicin-intercalated smectite as a new therapeutic agent against Helicobacter pylori in a mouse model
Article Snippet: From the fecal materials of each mouse, DNA was extracted using FastDNA® SPIN Kit (MP Biomedicals, Solon, OH, USA). .. The samples were lysed with FastPrep® Instruments and centrifuged, and DNA was isolated from the supernatant using the procedure of silica-based GENECLEAN® and SPIN filters (MP Biomedicals, Solon, OH, USA)( ). .. PCR amplification and 16S rRNA gene sequencingUsing the extracted metagenomics DNA as a template, PCR was performed for amplification of the V3-V4 regions of the bacterial 16S rRNA gene using the primers 341F (5′-TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-CCTACGGGNGGCWGCAG-3′; the underlined sequence indicates the target region primer) and 805R (5′-GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-ACTACHVGGGTATCTAATCC-3′).

Article Title: Efficacy and Gut Dysbiosis of Gentamicin-Intercalated Smectite as a New Therapeutic Agent against Helicobacter pylori in a Mouse Model
Article Snippet: From the fecal materials of each mouse group, DNA was extracted using the FastDNA® SPIN Kit (MP Biomedicals, Solon, OH, USA). .. The samples were lysed with FastPrep® Instruments and centrifuged, and DNA was isolated from the supernatant using the procedure of silica-based GENECLEAN® and SPIN filters (MP Biomedicals, Solon, OH, USA) [ ]. ..

Incubation:

Article Title: Cleaved amplified polymorphic sequences (CAPS) marker for identification of two mutant alleles of the rapeseed BnaA.FAD2 gene
Article Snippet: .. The suspension on the SPIN Filter was then resuspended with 100 μl of the distilled water (MP Biomedicals) and incubated for 5 min at 55 °C. ..