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Promega spin column clean up kit
Spin Column Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spin column clean up kit/product/Promega
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
spin column clean up kit - by Bioz Stars, 2020-04
93/100 stars

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perfluorooctanol

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Nucleic Acid Electrophoresis:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit). .. We performed a nested PCR amplification (Accuprime Pfx Supermix (Thermo Fisher)) in a total volume of 100 μl using 10 μl of emulsion product as template with 100 nM primers 5΄-AAT GAT ACG GCG ACC ACC GA-3΄ and 5΄- CAA GCA GAA GAC GGC ATA CGA-3΄ under the following conditions: 2 min 94°C; followed by 30 cycles of 94°C for 30 s, 62°C for 30 s and 72°C for 30 s; followed by a final extension at 72°C for 7 min. We purified the final product by gel electrophoresis and sequenced using the PE36 protocol on the MiSeq platform (Illumina).

Amplification:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We preformed PCR amplification with the following thermocycling conditions. .. We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit).

Polymerase Chain Reaction:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: .. We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit). .. We performed a nested PCR amplification (Accuprime Pfx Supermix (Thermo Fisher)) in a total volume of 100 μl using 10 μl of emulsion product as template with 100 nM primers 5΄-AAT GAT ACG GCG ACC ACC GA-3΄ and 5΄- CAA GCA GAA GAC GGC ATA CGA-3΄ under the following conditions: 2 min 94°C; followed by 30 cycles of 94°C for 30 s, 62°C for 30 s and 72°C for 30 s; followed by a final extension at 72°C for 7 min. We purified the final product by gel electrophoresis and sequenced using the PE36 protocol on the MiSeq platform (Illumina).

Purification:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: .. We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit). .. We performed a nested PCR amplification (Accuprime Pfx Supermix (Thermo Fisher)) in a total volume of 100 μl using 10 μl of emulsion product as template with 100 nM primers 5΄-AAT GAT ACG GCG ACC ACC GA-3΄ and 5΄- CAA GCA GAA GAC GGC ATA CGA-3΄ under the following conditions: 2 min 94°C; followed by 30 cycles of 94°C for 30 s, 62°C for 30 s and 72°C for 30 s; followed by a final extension at 72°C for 7 min. We purified the final product by gel electrophoresis and sequenced using the PE36 protocol on the MiSeq platform (Illumina).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: Paragraph title: HEK cell emulsion RT-PCR ... We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit).

Nested PCR:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit). .. We performed a nested PCR amplification (Accuprime Pfx Supermix (Thermo Fisher)) in a total volume of 100 μl using 10 μl of emulsion product as template with 100 nM primers 5΄-AAT GAT ACG GCG ACC ACC GA-3΄ and 5΄- CAA GCA GAA GAC GGC ATA CGA-3΄ under the following conditions: 2 min 94°C; followed by 30 cycles of 94°C for 30 s, 62°C for 30 s and 72°C for 30 s; followed by a final extension at 72°C for 7 min. We purified the final product by gel electrophoresis and sequenced using the PE36 protocol on the MiSeq platform (Illumina).

CTG Assay:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We made a 100 μl RT-PCR mixture containing: IP products (variable), 25 μl OneStep MasterMix, 5 μl primer 5΄-CAG CTC GAC CAG GAT GGG CA-3΄ (10 μM), 5 μl Primer 5΄-TTC AGC TTG GCG GTC TGG GT-3΄ (10 μM), 5 μl primer 5΄-TAT TCC CAT GGC GCG CCG CTG GTC GGT ACG GTA ACG GA-3΄ (1 μM), 5 μl primer 5΄-GGC GCG CCA TGG GAA TAC GGA CGA TGC CGT CCT CGT A-3΄ (1 μM), 20 μl 5% Tetronic and H2 O to 100 μl. .. We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit).

Chloramphenicol Acetyltransferase Assay:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We made a 100 μl RT-PCR mixture containing: IP products (variable), 25 μl OneStep MasterMix, 5 μl primer 5΄-CAG CTC GAC CAG GAT GGG CA-3΄ (10 μM), 5 μl Primer 5΄-TTC AGC TTG GCG GTC TGG GT-3΄ (10 μM), 5 μl primer 5΄-TAT TCC CAT GGC GCG CCG CTG GTC GGT ACG GTA ACG GA-3΄ (1 μM), 5 μl primer 5΄-GGC GCG CCA TGG GAA TAC GGA CGA TGC CGT CCT CGT A-3΄ (1 μM), 20 μl 5% Tetronic and H2 O to 100 μl. .. We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit).

Cellular Antioxidant Activity Assay:

Article Title: Using high-throughput barcode sequencing to efficiently map connectomes
Article Snippet: We broke the emulsions with perfluorooctanol, and collected the aqueous phase for subsequent purification using a spin-column clean-up kit (Promega Wizard SV PCR Cleanup Kit). .. We performed a nested PCR amplification (Accuprime Pfx Supermix (Thermo Fisher)) in a total volume of 100 μl using 10 μl of emulsion product as template with 100 nM primers 5΄-AAT GAT ACG GCG ACC ACC GA-3΄ and 5΄- CAA GCA GAA GAC GGC ATA CGA-3΄ under the following conditions: 2 min 94°C; followed by 30 cycles of 94°C for 30 s, 62°C for 30 s and 72°C for 30 s; followed by a final extension at 72°C for 7 min. We purified the final product by gel electrophoresis and sequenced using the PE36 protocol on the MiSeq platform (Illumina).

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    Promega spin column clean up kit
    Spin Column Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spin column clean up kit/product/Promega
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spin column clean up kit - by Bioz Stars, 2020-04
    93/100 stars
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