Structured Review

U73122 PLC ikk inhibitors
<t>IKK</t> inhibition reduced growth of V2D1 tumors (A) Blood from wt or DBA1- Rag1 −/− -mice was analyzed for CD3 and B220. (B) V2D1-cells (1 × 10 6 ) were injected subcutaneously into the flanks of DBA/1- Rag1 −/− -mice and tumor area was assessed for 6 weeks using a Mitutoyo Quick Mini caliper. Growing tumors were either treated with DMSO/PBS (blue line) or with IKK-inhibitor <t>VII</t> (red line) (25 μM). [Data represent the mean SD of 10 mice with tumors ( p
Ikk Inhibitors, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 89/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells"

Article Title: Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells

Journal: Oncotarget

doi:

IKK inhibition reduced growth of V2D1 tumors (A) Blood from wt or DBA1- Rag1 −/− -mice was analyzed for CD3 and B220. (B) V2D1-cells (1 × 10 6 ) were injected subcutaneously into the flanks of DBA/1- Rag1 −/− -mice and tumor area was assessed for 6 weeks using a Mitutoyo Quick Mini caliper. Growing tumors were either treated with DMSO/PBS (blue line) or with IKK-inhibitor VII (red line) (25 μM). [Data represent the mean SD of 10 mice with tumors ( p
Figure Legend Snippet: IKK inhibition reduced growth of V2D1 tumors (A) Blood from wt or DBA1- Rag1 −/− -mice was analyzed for CD3 and B220. (B) V2D1-cells (1 × 10 6 ) were injected subcutaneously into the flanks of DBA/1- Rag1 −/− -mice and tumor area was assessed for 6 weeks using a Mitutoyo Quick Mini caliper. Growing tumors were either treated with DMSO/PBS (blue line) or with IKK-inhibitor VII (red line) (25 μM). [Data represent the mean SD of 10 mice with tumors ( p

Techniques Used: Inhibition, Mouse Assay, Injection

The IL-3-induced IKK2 activation mediates mitogenic signaling (A, B) BMMCs were pre-incubated with the IKK-inhibitor VII and were stimulated with IL-3. Lysates were analyzed by westernblotting (A) or cells were probed with [H 3 ]thymidine and analyzed by β -counting (B) ( p
Figure Legend Snippet: The IL-3-induced IKK2 activation mediates mitogenic signaling (A, B) BMMCs were pre-incubated with the IKK-inhibitor VII and were stimulated with IL-3. Lysates were analyzed by westernblotting (A) or cells were probed with [H 3 ]thymidine and analyzed by β -counting (B) ( p

Techniques Used: Activation Assay, Incubation

Survival of V2D1 cells depends on the IKK-mediated IL-3 production (A) V2D1 cells were cultured in IL-3-free medium for different time periods. Lysates were analyzed by westernblotting. (B–D) V2D1 cells were treated with the IKK-inhibitor VII. Lysates were analyzed by westernblotting (B) or cells were probed with [H 3 ]thymidine (C) or PI (D) . Cells were analyzed by β -counting (C) or by flow cytometry (D) ( C ; p
Figure Legend Snippet: Survival of V2D1 cells depends on the IKK-mediated IL-3 production (A) V2D1 cells were cultured in IL-3-free medium for different time periods. Lysates were analyzed by westernblotting. (B–D) V2D1 cells were treated with the IKK-inhibitor VII. Lysates were analyzed by westernblotting (B) or cells were probed with [H 3 ]thymidine (C) or PI (D) . Cells were analyzed by β -counting (C) or by flow cytometry (D) ( C ; p

Techniques Used: Cell Culture, Flow Cytometry, Cytometry

The IL3-induced IKK activation does not mediate IκBα degradation (A, B) BMMCs were stimulated with IL-3 (A, B) or IL-33 (B) Lysates were analyzed by westernblotting. (C) BMMCs were stimulated with IL-3 or IL-33. Supernatants were collected and analyzed for IL-6. (D–F) NFκB-EGFP-MC/9 cells were pre-incubated with the IKK-inhibitor VII and stimulated with IL-3 or IL-33. Lysates were analyzed by westernblotting (D) or cells were analyzed for EGFP-production by flow cytometry (E) or collected supernatants were analyzed for IL-6 (F)
Figure Legend Snippet: The IL3-induced IKK activation does not mediate IκBα degradation (A, B) BMMCs were stimulated with IL-3 (A, B) or IL-33 (B) Lysates were analyzed by westernblotting. (C) BMMCs were stimulated with IL-3 or IL-33. Supernatants were collected and analyzed for IL-6. (D–F) NFκB-EGFP-MC/9 cells were pre-incubated with the IKK-inhibitor VII and stimulated with IL-3 or IL-33. Lysates were analyzed by westernblotting (D) or cells were analyzed for EGFP-production by flow cytometry (E) or collected supernatants were analyzed for IL-6 (F)

Techniques Used: Activation Assay, Incubation, Flow Cytometry, Cytometry

2) Product Images from "Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway"

Article Title: Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21114069

Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p
Figure Legend Snippet: Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p

Techniques Used: Western Blot

3) Product Images from "Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway"

Article Title: Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21114069

Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p
Figure Legend Snippet: Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p

Techniques Used: Western Blot

4) Product Images from "Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway"

Article Title: Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21114069

Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p
Figure Legend Snippet: Apelin-13 ameliorates MGO-induced apoptosis through AMPK pathway in HUVECs. Cells pretreated with wortmannin (50 nM), U73122 (1 µM), and compound C (1 µM) were stimulated with 100 µM MGO for 24 h in the presence or absence of 1 µM apelin-13. ( A ) HUVECs were lysed and then applied for Western blotting analysis. Protein level amounts were determined by immunoblotting with anti-PARP-1 and anti-caspase-3 antibodies. The graph shows the densitometric quantification of Western blot bands. ** p

Techniques Used: Western Blot

Related Articles

Lysis:

Article Title: Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells
Article Snippet: .. Stimulation and lysis BMMCs (106 cells/ml) were IL-3-starved (1 h), pre-incubated (30 min) with SP600125 (JNK-inhibitor), SU6656 (SFK-inhibitor), IKK-inhibitors (VII, PS-1145, BMS-34554) and U73122 (PLC inhibitor) (If not other stated all these inhibitors were used in a concentration of 5 μM). .. Furthermore we used the protein biosynthesis inhibitor cyclohexamide (340 μM), the PLD1 inhibitor CAY10594 (10 μM), and the Ca2+ chelator BAPTA-AM (10 μM) or ionomycin (in a concentration from 0,5–10 ng/ml) (all inhibitors were from Merck Millipore).

Inhibition:

Article Title: Apelin-13 Inhibits Methylglyoxal-Induced Unfolded Protein Responses and Endothelial Dysfunction via Regulating AMPK Pathway
Article Snippet: .. Apelin-13 Ameliorates MGO-Induced Endothelial Apoptosis through AMPK PathwayTo identify the molecular mechanism by which apelin-13 regulates the inhibition of MGO-induced apoptosis, HUVECs were pretreated with wortmannin (PI3K inhibitor), U73122 (PLC inhibitor), and compound C (AMPK inhibitor) upon stimulation with MGO in the presence of apelin-13. .. As shown in A, apelin-mediated inhibition of MGO-induced apoptosis was impaired by compound C, but not by wortmannin and U73122.

Cell Culture:

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23
Article Snippet: .. Various doses of FGF2 (0∼100 ng/ml) or FGF2 (50 ng/ml) in the presence and absence of Wortmannin (PI3K inhibitor, 1.0 µM), various doses of U73122 (PLC inhibitor, 1∼10 µM), and various doses of U0126 (MEK inhibitor, 5∼20 µM) were added to the cell culture media for 24 hours before cells were harvested. .. Cells were lysed in 50 µl of reporter lysis buffer (Promega, Madison, WI).

Concentration Assay:

Article Title: Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells
Article Snippet: .. Stimulation and lysis BMMCs (106 cells/ml) were IL-3-starved (1 h), pre-incubated (30 min) with SP600125 (JNK-inhibitor), SU6656 (SFK-inhibitor), IKK-inhibitors (VII, PS-1145, BMS-34554) and U73122 (PLC inhibitor) (If not other stated all these inhibitors were used in a concentration of 5 μM). .. Furthermore we used the protein biosynthesis inhibitor cyclohexamide (340 μM), the PLD1 inhibitor CAY10594 (10 μM), and the Ca2+ chelator BAPTA-AM (10 μM) or ionomycin (in a concentration from 0,5–10 ng/ml) (all inhibitors were from Merck Millipore).

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    U73122 PLC sp600125 jnk inhibitor
    Sp600125 Jnk Inhibitor, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sp600125 jnk inhibitor/product/U73122 PLC
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    sp600125 jnk inhibitor - by Bioz Stars, 2020-11
    89/100 stars
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