Structured Review

u73122 plc sp600125
Sp600125, supplied by u73122 plc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sp600125/product/u73122 plc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sp600125 - by Bioz Stars, 2021-05
86/100 stars

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Related Articles

Lysis:

Article Title: Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells
Article Snippet: After harvesting and washing (PBA-buffer; 0.25% BSA and 0.02% sodium azide in PBS) cells were analyzed by using an LSR II flow cytometer (BD) and FlowJo 8.1.1 (Treestar Inc.). .. Stimulation and lysis BMMCs (106 cells/ml) were IL-3-starved (1 h), pre-incubated (30 min) with SP600125 (JNK-inhibitor), SU6656 (SFK-inhibitor), IKK-inhibitors (VII, PS-1145, BMS-34554) and U73122 (PLC inhibitor) (If not other stated all these inhibitors were used in a concentration of 5 μM). .. Furthermore we used the protein biosynthesis inhibitor cyclohexamide (340 μM), the PLD1 inhibitor CAY10594 (10 μM), and the Ca2+ chelator BAPTA-AM (10 μM) or ionomycin (in a concentration from 0,5–10 ng/ml) (all inhibitors were from Merck Millipore).

Concentration Assay:

Article Title: Subthreshold IKK activation modulates the effector functions of primary mast cells and allows specific targeting of transformed mast cells
Article Snippet: After harvesting and washing (PBA-buffer; 0.25% BSA and 0.02% sodium azide in PBS) cells were analyzed by using an LSR II flow cytometer (BD) and FlowJo 8.1.1 (Treestar Inc.). .. Stimulation and lysis BMMCs (106 cells/ml) were IL-3-starved (1 h), pre-incubated (30 min) with SP600125 (JNK-inhibitor), SU6656 (SFK-inhibitor), IKK-inhibitors (VII, PS-1145, BMS-34554) and U73122 (PLC inhibitor) (If not other stated all these inhibitors were used in a concentration of 5 μM). .. Furthermore we used the protein biosynthesis inhibitor cyclohexamide (340 μM), the PLD1 inhibitor CAY10594 (10 μM), and the Ca2+ chelator BAPTA-AM (10 μM) or ionomycin (in a concentration from 0,5–10 ng/ml) (all inhibitors were from Merck Millipore).

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    u73122 plc sp600125
    Sp600125, supplied by u73122 plc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sp600125/product/u73122 plc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sp600125 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

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