solid pin multi blot replicators  (AutoMate Scientific Inc)

Journal: STAR Protocols

Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

doi: 10.1016/j.xpro.2021.100868

Figure Lengend Snippet: Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

Techniques: Mutagenesis, Expressing, Flow Cytometry

Journal: STAR Protocols

Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

doi: 10.1016/j.xpro.2021.100868

Figure Lengend Snippet:

Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

Techniques: Recombinant, Clone Assay, Software, Microscopy