sodium pyrophosphate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sodium pyrophosphate
    Sodium Pyrophosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Thermo Fisher
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    99/100 stars

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    Centrifugation:

    Article Title: c-Src functionality controls self-renewal and glucose metabolism in MCF7 breast cancer stem cells
    Article Snippet: .. Briefly, mammospheres were collected by centrifugation at 200xg 5 min at room temperature, washed twice with cool PBS and lysed at 4°C with lysis buffer [10 mM Tris–HCl (pH 7.6), 50 mM NaCl, 30 mM sodium pyrophosphate, 5 mM EDTA, 5 mM EGTA, 0.1% SDS, 1% Triton X-100, 50 mM NaF, 0.1 mM Na3 VO4, 1 mM PMSF, 1 mM benzamidine, 1 mM iodoacetamide and 1 mM phenantroline]. .. Cell lysates were obtained by centrifugation at 21,380xg for 30 min at 4°C; protein concentration in the supernatant was determined by BCA protein assay (Pierce, Rockford, IL), and lysates were adjusted to equivalent concentrations with lysis buffer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in kidney epithelial cells
    Article Snippet: .. Immunoblot analysis and ELISA Whole-cell lysates from renal cortical tissues were prepared using modified RIPA buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% NP-40, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, protease, and phosphatase inhibitors) supplemented with 1% SDS. .. Invitrogen Bis-tris gradient midi-gels were used for western blot analysis, followed by detection by ECL reagent (Cell Signaling).

    Incubation:

    Article Title: Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study.
    Article Snippet: .. Various mechanisms, including oxidative stress, inflammation, and protease-antiprotease imbalance are proposed for the progressive decline in lung function in chronic obstructive pulmonary disease (COPD). .. Various mechanisms, including oxidative stress, inflammation, and protease-antiprotease imbalance are proposed for the progressive decline in lung function in chronic obstructive pulmonary disease (COPD).

    Sonication:

    Article Title: Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target
    Article Snippet: .. Cell lysis and Protein Digestion Cells were lysed in lysis buffer (50 mM triethyl ammonium bicarbonate (TEABC) pH 8.0, 2% SDS, 1 mM sodium orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium fluoride), sonicated and centrifuged at 16,000× g for 20 min. .. Protein estimation of the clarified lysate was done using bicinchoninic acid assay (BCA) (Pierce, Waltham, MA, USA) according to manufacturer’s instructions.

    Modification:

    Article Title: SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in kidney epithelial cells
    Article Snippet: .. Immunoblot analysis and ELISA Whole-cell lysates from renal cortical tissues were prepared using modified RIPA buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% NP-40, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, protease, and phosphatase inhibitors) supplemented with 1% SDS. .. Invitrogen Bis-tris gradient midi-gels were used for western blot analysis, followed by detection by ECL reagent (Cell Signaling).

    Lysis:

    Article Title: Molecular basis for substrate recruitment to the PRMT5 methylosome
    Article Snippet: .. The remaining cell pellets were lysed in PTMScan® Urea Lysis Buffer (20 mM HEPES (pH 8.0), 9.0 M urea, 1 mM sodium orthovanadate (activated), 2.5 mM sodium pyrophosphate, and 1 mM β-glycerol-phosphate). .. Protein concentration was determined by Bradford assay and 2.8 mg of cell lysate was used from each sample for subsequent analyses.

    Article Title: Pretreatment with LCK Inhibitors Chemosensitizes Cisplatin-Resistant Endometrioid Ovarian Tumors
    Article Snippet: .. 2.3 Immunoblotting Protein lysates were obtained with cell lysis in 20mM Tris-HCl (pH 7.5), 150mM NaCl, 1 mM Na2EDTA, 1% NP-40, 1 mM EGTA, 1% sodium pyrophosphate, 1 mM β-glycerophosphate, 1mM sodium orthovanadate, 1 μ g/mL leupeptin, 20 mM NaF and 1 mM PMSF. .. Protein concentrations were measured with BCA Protein Assay Kit (ThermoFisher Scientific).

    Article Title: c-Src functionality controls self-renewal and glucose metabolism in MCF7 breast cancer stem cells
    Article Snippet: .. Briefly, mammospheres were collected by centrifugation at 200xg 5 min at room temperature, washed twice with cool PBS and lysed at 4°C with lysis buffer [10 mM Tris–HCl (pH 7.6), 50 mM NaCl, 30 mM sodium pyrophosphate, 5 mM EDTA, 5 mM EGTA, 0.1% SDS, 1% Triton X-100, 50 mM NaF, 0.1 mM Na3 VO4, 1 mM PMSF, 1 mM benzamidine, 1 mM iodoacetamide and 1 mM phenantroline]. .. Cell lysates were obtained by centrifugation at 21,380xg for 30 min at 4°C; protein concentration in the supernatant was determined by BCA protein assay (Pierce, Rockford, IL), and lysates were adjusted to equivalent concentrations with lysis buffer.

    Article Title: Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target
    Article Snippet: .. Cell lysis and Protein Digestion Cells were lysed in lysis buffer (50 mM triethyl ammonium bicarbonate (TEABC) pH 8.0, 2% SDS, 1 mM sodium orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium fluoride), sonicated and centrifuged at 16,000× g for 20 min. .. Protein estimation of the clarified lysate was done using bicinchoninic acid assay (BCA) (Pierce, Waltham, MA, USA) according to manufacturer’s instructions.

    Article Title: mTORC1 inhibitor RAD001 (everolimus) enhances non-small cell lung cancer cell radiosensitivity in vitro via suppressing epithelial–mesenchymal transition
    Article Snippet: .. Cells were collected, washed twice in ice-cold phosphate-buffered saline (PBS) and lysed in ice-cold lysis buffer containing 20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 mM EDTA, and 1 μg/mL leupeptin with 1 mM PMSF and 1 mM phosphatase inhibitors. .. Before analysis, protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Genetic loss of AMPK-glycogen binding destabilises AMPK and disrupts metabolism
    Article Snippet: .. To generate whole liver, muscle and adipose tissue lysates, 40 mg of frozen tissue was homogenised in lysis buffer containing 50 mM of Tris.HCl (pH 7.5), 1 mM of EDTA, 1 mM of EGTA, 10% glycerol, 1% Triton X-100, 50 mM of sodium fluoride, 5 mM of sodium pyrophosphate, 1 mM of DTT, 10 μg/ml of trypsin inhibitor, 2 μg/ml of aprotinin, 1 mM of benzamidine, and 1 mM of PMSF. .. Samples were centrifuged at 16,000 g for 30 min at 4 °C to remove cellular debris, and protein concentration was determined via bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL, USA; 23,227).

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    Thermo Fisher lysis buffer
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    Thermo Fisher sodium pyrophosphate nappi
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    Thermo Fisher sodium pyrophosphate tetrabasic
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    https://www.bioz.com/result/sodium pyrophosphate tetrabasic/product/Thermo Fisher
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