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Santa Cruz Biotechnology sodium pyrophosphate
Sodium Pyrophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium pyrophosphate/product/Santa Cruz Biotechnology
Average 92 stars, based on 12 article reviews
Price from $9.99 to $1999.99
sodium pyrophosphate - by Bioz Stars, 2020-09
92/100 stars

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Immunoprecipitation:

Article Title: Oxidative stress regulates IGF1R expression in vascular smooth-muscle cells via p53 and HDAC recruitment
Article Snippet: .. VSMCs were harvested in immunoprecipitation buffer [20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.1% (v/v) Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerol phosphate and 1 mM sodium orthovanadate] and 600 μg of pre-cleared protein lysate was incubated with 2.5 μg of rabbit anti-TBP [TATA-box-binding protein, also known as TFIID (transcription factor IID)] antibody (Santa Cruz Biotechnology) or mouse anti-p53 antibody (Santa Cruz Biotechnology) for 16 h at 4 °C. .. In some instances, the pre-cleared lysate was incubated with 20 units of calf intestinal phosphatase for 30 min at 37 °C prior to the addition of the anti-TBP antibody.

Article Title: Human Immunodeficiency Virus Type 1 Tat Regulates Endothelial Cell Actin Cytoskeletal Dynamics through PAK1 Activation and Oxidant Production
Article Snippet: .. After treatment, HUVEC were harvested in cold lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM disodium EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 μg of leupeptin/ml, and 1 mM phenylmethylsulfonyl fluoride), sonicated briefly, and immunoprecipitated with rabbit anti-PAK1 (Santa Cruz). .. The kinase reaction was performed with 5 μg of myelin basic protein (Upstate) as a PAK1 substrate in the presence of [γ32 P]ATP (Perkin-Elmer) at 30°C for 30 min. For JNK activity, HUVEC were cotransfected with HA-JNK2 and either PAK1(K298A) or empty vector.

Protease Inhibitor:

Article Title: PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise
Article Snippet: .. Briefly, frozen tissues were powdered and then homogenized in a 15:1 (v/w) ratio of ice-cold RIPA buffer (50 mM Tris-HCl (pH 7.4); 10% Nonidet P-40; 0.25% sodium deoxycholate; 150 mM NaCl; 1 mM EDTA; 0.5 M NaF; 10mM sodium pyrophosphate) supplemented with protease inhibitor cocktail tablet (Santa Cruz Biotechnology #SC-29131, Santa Cruz, CA, USA). .. Protein concentration was measured by the method of , of and ample volumes were adjusted with lane marker reducing sample buffer (Thermo fisher scientific #39000, Houston, TX, USA) to load the same protein concentration, and proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes, then blocked for 1 hr in 5% w/v none-fat milk in TBS-T (tris-buffered saline (TBS) containing 0.1% tween 20 (Sigma-aldrich #P1379, St. Louis, MO, USA) and 0.2% imidazole (Sigma-aldrich #56750, St. Louis, MO, USA)).

Article Title: ROCK Inhibition Activates MCF-7 Cells
Article Snippet: .. Immunoblotting The cells were lysed with a lysis buffer (Cell Signaling Technology, Beverly, MA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3 VO4 , 1 µg/ml leupeptin, 1 mM PMSF, and a complete protease inhibitor cocktail tablet (Santa Cruz Biotechnology, Santa Cruz, CA). .. After high-speed centrifugation of the cell lysates, the protein concentration of the supernatants was determined using the Bradford assay (Bio-Rad, Hercules, CA).

Article Title: Hippo signaling is intrinsically regulated during cell cycle progression by APC/CCdh1
Article Snippet: .. Cells were prepared using a lysis buffer [20 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium orthovanadate] or RIPA buffer (Santa Cruz Biotechnology), respectively, containing protease inhibitor mixture (Roche). .. Immnoprecipitates or total cell lysates were analyzed by Western blotting according to standard procedures.

Article Title: Magnesium isoglycyrrhizinate ameliorates doxorubicin-induced acute cardiac and hepatic toxicity via anti-oxidant and anti-apoptotic mechanisms in mice
Article Snippet: .. The samples were homogenized and lysed with RIPA lysis buffer containing 50 mM Tris-HCl, 125 mM sodium chloride, 5 mM sodium pyrophosphate, 50 mM sodium fluoride, 1 mM EDTA, 1 mM dithiothreitol, 0.1% SDS (w/v), 1% TritonX-100 (v/v) and protease inhibitor cocktail (all Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. After incubation for 20 min on ice, the lysis buffer solution was centrifuged at 4°C at 7,800 × g for 20 min.

Indirect Immunoperoxidase Assay:

Article Title: Isopropylamine NONOate (IPA/NO) Moderates Neointimal Hyperplasia Following Vascular Injury
Article Snippet: .. For immunoprecipitations, IPA/NO-treated and untreated VSMC were lysed using lysis buffer A (20 mmol/L Tris-HCl [pH 7.5], 150 mmol/L NaCl, 1 mmol/L ethylenediaminetetraacetic acid (EDTA), 1 mmol/L ethyleneglycoltetraacetic acid (EGTA), 1% (v/v) Triton X-100, 2.5 mmol/L sodium pyrophosphate, 1 mmol/L β-glycerol phosphate, 1 mmol/L sodium orthovanadate, 1 μg/mL leupeptin, and 1 mmol/L PMSF), and 300 μg of lysate was incubated with 1 μg of rabbit IgG plus 20 μL of protein A/G sepharose (both from Santa Cruz) for 1 hour at 4°C on a rotating wheel. .. The beads were then pelleted by centrifugation for 5 minutes at 1500 rpm and 4°C in an Eppendorf 5417R centrifuge (Westbury, NY), and the supernatant transferred to a fresh tube, to which was added 20 μL of protein A/G sepharose and 2.5 μg of either cdc2, cdk2, cdk4 or cdk6 antibody.

Incubation:

Article Title: Isopropylamine NONOate (IPA/NO) Moderates Neointimal Hyperplasia Following Vascular Injury
Article Snippet: .. For immunoprecipitations, IPA/NO-treated and untreated VSMC were lysed using lysis buffer A (20 mmol/L Tris-HCl [pH 7.5], 150 mmol/L NaCl, 1 mmol/L ethylenediaminetetraacetic acid (EDTA), 1 mmol/L ethyleneglycoltetraacetic acid (EGTA), 1% (v/v) Triton X-100, 2.5 mmol/L sodium pyrophosphate, 1 mmol/L β-glycerol phosphate, 1 mmol/L sodium orthovanadate, 1 μg/mL leupeptin, and 1 mmol/L PMSF), and 300 μg of lysate was incubated with 1 μg of rabbit IgG plus 20 μL of protein A/G sepharose (both from Santa Cruz) for 1 hour at 4°C on a rotating wheel. .. The beads were then pelleted by centrifugation for 5 minutes at 1500 rpm and 4°C in an Eppendorf 5417R centrifuge (Westbury, NY), and the supernatant transferred to a fresh tube, to which was added 20 μL of protein A/G sepharose and 2.5 μg of either cdc2, cdk2, cdk4 or cdk6 antibody.

Article Title: Oxidative stress regulates IGF1R expression in vascular smooth-muscle cells via p53 and HDAC recruitment
Article Snippet: .. VSMCs were harvested in immunoprecipitation buffer [20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.1% (v/v) Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerol phosphate and 1 mM sodium orthovanadate] and 600 μg of pre-cleared protein lysate was incubated with 2.5 μg of rabbit anti-TBP [TATA-box-binding protein, also known as TFIID (transcription factor IID)] antibody (Santa Cruz Biotechnology) or mouse anti-p53 antibody (Santa Cruz Biotechnology) for 16 h at 4 °C. .. In some instances, the pre-cleared lysate was incubated with 20 units of calf intestinal phosphatase for 30 min at 37 °C prior to the addition of the anti-TBP antibody.

Lysis:

Article Title: ROCK Inhibition Activates MCF-7 Cells
Article Snippet: .. Immunoblotting The cells were lysed with a lysis buffer (Cell Signaling Technology, Beverly, MA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3 VO4 , 1 µg/ml leupeptin, 1 mM PMSF, and a complete protease inhibitor cocktail tablet (Santa Cruz Biotechnology, Santa Cruz, CA). .. After high-speed centrifugation of the cell lysates, the protein concentration of the supernatants was determined using the Bradford assay (Bio-Rad, Hercules, CA).

Article Title: Isopropylamine NONOate (IPA/NO) Moderates Neointimal Hyperplasia Following Vascular Injury
Article Snippet: .. For immunoprecipitations, IPA/NO-treated and untreated VSMC were lysed using lysis buffer A (20 mmol/L Tris-HCl [pH 7.5], 150 mmol/L NaCl, 1 mmol/L ethylenediaminetetraacetic acid (EDTA), 1 mmol/L ethyleneglycoltetraacetic acid (EGTA), 1% (v/v) Triton X-100, 2.5 mmol/L sodium pyrophosphate, 1 mmol/L β-glycerol phosphate, 1 mmol/L sodium orthovanadate, 1 μg/mL leupeptin, and 1 mmol/L PMSF), and 300 μg of lysate was incubated with 1 μg of rabbit IgG plus 20 μL of protein A/G sepharose (both from Santa Cruz) for 1 hour at 4°C on a rotating wheel. .. The beads were then pelleted by centrifugation for 5 minutes at 1500 rpm and 4°C in an Eppendorf 5417R centrifuge (Westbury, NY), and the supernatant transferred to a fresh tube, to which was added 20 μL of protein A/G sepharose and 2.5 μg of either cdc2, cdk2, cdk4 or cdk6 antibody.

Article Title: Hippo signaling is intrinsically regulated during cell cycle progression by APC/CCdh1
Article Snippet: .. Cells were prepared using a lysis buffer [20 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium orthovanadate] or RIPA buffer (Santa Cruz Biotechnology), respectively, containing protease inhibitor mixture (Roche). .. Immnoprecipitates or total cell lysates were analyzed by Western blotting according to standard procedures.

Article Title: Magnesium isoglycyrrhizinate ameliorates doxorubicin-induced acute cardiac and hepatic toxicity via anti-oxidant and anti-apoptotic mechanisms in mice
Article Snippet: .. The samples were homogenized and lysed with RIPA lysis buffer containing 50 mM Tris-HCl, 125 mM sodium chloride, 5 mM sodium pyrophosphate, 50 mM sodium fluoride, 1 mM EDTA, 1 mM dithiothreitol, 0.1% SDS (w/v), 1% TritonX-100 (v/v) and protease inhibitor cocktail (all Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. After incubation for 20 min on ice, the lysis buffer solution was centrifuged at 4°C at 7,800 × g for 20 min.

Article Title: Human Immunodeficiency Virus Type 1 Tat Regulates Endothelial Cell Actin Cytoskeletal Dynamics through PAK1 Activation and Oxidant Production
Article Snippet: .. After treatment, HUVEC were harvested in cold lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM disodium EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 μg of leupeptin/ml, and 1 mM phenylmethylsulfonyl fluoride), sonicated briefly, and immunoprecipitated with rabbit anti-PAK1 (Santa Cruz). .. The kinase reaction was performed with 5 μg of myelin basic protein (Upstate) as a PAK1 substrate in the presence of [γ32 P]ATP (Perkin-Elmer) at 30°C for 30 min. For JNK activity, HUVEC were cotransfected with HA-JNK2 and either PAK1(K298A) or empty vector.

Sonication:

Article Title: Human Immunodeficiency Virus Type 1 Tat Regulates Endothelial Cell Actin Cytoskeletal Dynamics through PAK1 Activation and Oxidant Production
Article Snippet: .. After treatment, HUVEC were harvested in cold lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM disodium EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 μg of leupeptin/ml, and 1 mM phenylmethylsulfonyl fluoride), sonicated briefly, and immunoprecipitated with rabbit anti-PAK1 (Santa Cruz). .. The kinase reaction was performed with 5 μg of myelin basic protein (Upstate) as a PAK1 substrate in the presence of [γ32 P]ATP (Perkin-Elmer) at 30°C for 30 min. For JNK activity, HUVEC were cotransfected with HA-JNK2 and either PAK1(K298A) or empty vector.

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    Santa Cruz Biotechnology sodium pyrophosphate
    Sodium Pyrophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Santa Cruz Biotechnology
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    92/100 stars
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