Structured Review

Merck & Co sodium pyrophosphate
Sodium Pyrophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium pyrophosphate/product/Merck & Co
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium pyrophosphate - by Bioz Stars, 2020-09
92/100 stars

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Concentration Assay:

Article Title: High-Level Expression of AmpC ?-Lactamase Due to Insertion of Nucleotides between -10 and -35 Promoter Sequences in Escherichia coli Clinical Isolates: Cases Not Responsive to Extended-Spectrum-Cephalosporin Treatment
Article Snippet: .. The extension reaction was performed at 42°C for 1 h after the addition of sodium pyrophosphate (final concentration, 4 mM; Merck & Co., Inc., Rahway, N.J.), deoxynucleoside triphosphates (1 mM; Amersham-Pharmacia Biotech Inc.), and 30 U of avian myeloblastosis virus reverse transcriptase (Promega). .. The marker was generated by PCR with the same primer used for extension in the sequencing reaction (Sequenase [version 2.0] DNA sequencing kit; United States Biochemical Corp., Cleveland, Ohio) of a complete ampC gene (bp 10180 to 5316 of the sequence with GenBank accession no. AE000487 ) cloned into the pGEMT-EASY vector (Promega).

Protease Inhibitor:

Article Title: FASN Is a Biomarker Enriched in Malignant Glioma-Derived Extracellular Vesicles
Article Snippet: .. Immunoblot Analysis Cells and EVs were lysed using RIPA buffer, containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, aprotinin (10 mg/mL), 1 mM phenylmethylsulfonyl fluoride, leupeptin (10 mg/mL), 2 mM Na3 VO4 , 4 mM EDTA, 10 mM NaF, 10 mM sodium pyrophosphate, 1% NP-40, 0.1% sodium deoxycholate and 1% protease inhibitor (Merck). .. Total protein concentration was measured using the BCA assay.

Hybridization:

Article Title: Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
Article Snippet: .. Two smears of each sample were covered with approximately 20 μl of the fluorescein-labelled MTC-specific PNA probe (100 nM) or the fluorescein-labelled NTM-specific PNA probe (25 nM) in a hybridization solution containing 10% (wt/vol) dextran sulfate (Sigma Chemical Co., St. Louis, Mo.), 10 mM NaCl (Merck, Darmstadt, Germany), 30% (vol/vol) formamide (Life Technologies, Gaithersburg, Md.), 0.1% (wt/vol) sodium pyrophosphate (Merck), 0.2% (wt/vol) polyvinylpyrrolidone (Sigma Chemical Co.), 0.2% (wt/vol) Ficoll (Sigma Chemical Co.), 5 mM disodium EDTA (Merck), 0.1% (vol/vol) Triton X-100 (Serva, Heidelberg, Germany), and 50 mM Tris-HCl (pH 7.5). .. To avoid unspecific binding of the labelled PNA probes, 1 μM nonlabelled, nontarget PNA probe was included in the hybridization solution.

other:

Article Title: Pyrene removal from contaminated soils by modified Fenton oxidation using iron nano particles
Article Snippet: Hydrogen peroxide (30%), H2 SO4 (98%), HCl, NaOH, EDTA, sodium citrate, sodium pyrophosphate, HgCl2 , FeSO4 .7H2 O were also purchased from Merck Company.

Article Title: Administration of Ginkgo biloba Extract (EGb761) Alone and in Combination with FK506 Promotes Liver Regeneration in a Rat Model of Partial Hepatectomy
Article Snippet: Chemicals Reduced glutathione, NADH disodium salt, nitrobluetetrazolium, phenazine methosulphate, thiobarbituric acid, p-nitrophenyl phosphate, trichloroacetic acid, chloroform, sodium pyrophosphate, Triton X-100, hydrogen peroxide, and methanol were procured from Merck Specialties and Sigma-Aldrich Chemicals (St. Louis, MO).

Article Title: Natural Transformation of Acinetobacter sp. Strain BD413 with Cell Lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in Soil Microcosms
Article Snippet: The cores of the soil microcosms (approximately 25% of the total dry weight of the soil) were collected by using the wide end of a 1-ml Eppendorf tip, transferred to a 1.5-ml Eppendorf tube with 0.9 ml of 0.1% sodium pyrophosphate (tetrasodium diphosphate decahydrate; Merck) supplemented with 50 μl of DNase I (5 mg ml−1 ) and Vortex mixed with 0.2 g of sterile gravel (2- to 4-mm diameter).

Lysis:

Article Title: MLL fusion-driven activation of CDK6 potentiates proliferation in MLL-rearranged infant ALL
Article Snippet: .. After thawing, cells were resuspended in 50 μL of lysis buffer containing: 5 mM EDTA, 10% glycerol, 10 mM sodium pyrophosphate (Merck), 25 mM Tris, 150 mM NaCl, 1% Triton X-100, 1 mM sodium orthovanadate, 10 mM glycerolphosphate, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 1% aprotinin, 10 mM sodium fluoride (Sigma-Aldrich), and 50 μM of freshly prepared sodium pervanadate. .. Protein concentration was determined by BCA protein assay (Pierce Biotechnology, #23225) with different concentrations of bovine serum albumin as standards.

Article Title: Microfluidic Probe for Single-Cell Lysis and Analysis in Adherent Tissue Culture
Article Snippet: .. Buffer conditions Lysis buffer contained 50 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 30 mM sodium fluoride, 50 mM Tris buffer, pH 7.4; 1% Triton X-100 detergent, 15 mM NaCl, 2 mM EGTA, 100 μM sodium orthovanadate, 1 mM DTT, and 1X protease and phosphatase inhibitor cocktails (Merck/EMD 539134 and Sigma P2850, respectively). μ20 g/mL Alexa 488-labeled BSA was added to the lysis buffer to allow visualization of the lysis zone outside the microfluidic probe tip. .. Kinase activity assay conditions were 50 mM Tris-HCl (pH 7.3), 10 mM MgCl2 , 1 mM EGTA, 2 mM DTT, 4 μM PKC inhibitor peptide, 10 μM substrate peptide, 1 mM ATP (low metals grade; Calbiochem), 0.4 μM PKItide, and 5 μM and 25 μM GF109203X (EMD) for Akt and MK2, respectively (as in Shults et al, except without the inclusion of calmidazolium, as it was observed to cause problems in the device, possibly from surface binding).

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    https://www.bioz.com/result/sodium pyrophosphate/product/Merck & Co
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    Price from $9.99 to $1999.99
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    92/100 stars
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