Structured Review

Avantor sodium pyrophosphate
Sodium Pyrophosphate, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium pyrophosphate/product/Avantor
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
sodium pyrophosphate - by Bioz Stars, 2020-09
93/100 stars

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Protease Inhibitor:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Protein Concentration:

Article Title: Flow cytometric determination of genome size in European sunbleak Leucaspius delineatus (Heckel, 1843)
Article Snippet: .. Protein concentration analysis Blood cells and liver fragments isolated from L. delineatus were lysed for 30 min at 4°C with a buffer containing Tris–HCl, pH 7.5 (20 mM, Merck, Germany), sodium chloride (150 mM, Chempur, Poland), Na2 EDTA (1 mM, Sigma–Aldrich, Germany), EGTA (1 mM, Sigma, Germany), 1% Triton X-100 (Calbiochem, USA), sodium pyrophosphate (2.5 mM, POCH, Poland), β-glycerophosphate (1 mM, Laboratory BDH Reagent, GB). .. Then, the samples were mechanically homogenized and total protein content was estimated using a Micro BCA Protein Kit (Thermo Scientific, USA).

Concentration Assay:

Article Title: Metabolic Activity and Functional Diversity Changes in Sediment Prokaryotic Communities Organically Enriched with Mussel Biodeposits
Article Snippet: .. Briefly, 35 mL of seawater (0.2 μm filtered) and sodium pyrophosphate (5mM final concentration) were added to the tubes with sediments, which were then vortexed for 10 seconds and sonicated for five minutes using an ultrasonic bath (VWR Symphony). ..

Lysis:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Sonication:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Article Title: Metabolic Activity and Functional Diversity Changes in Sediment Prokaryotic Communities Organically Enriched with Mussel Biodeposits
Article Snippet: .. Briefly, 35 mL of seawater (0.2 μm filtered) and sodium pyrophosphate (5mM final concentration) were added to the tubes with sediments, which were then vortexed for 10 seconds and sonicated for five minutes using an ultrasonic bath (VWR Symphony). ..

Cell Surface Hydrophobicity:

Article Title: Cytocompatibility of the selected calcium phosphate based bone cements: comparative study in human cell culture
Article Snippet: .. Materials A1 and A2 were manufactured from powder composed of magnesium phosphate cement (MPC), hydroxyapatite (HA) and CSH, (all from Across Organics, USA) or sodium pyrophosphate (Na4 P2 O7 ·10H2 O, POCH, Poland). ..

Isolation:

Article Title: Flow cytometric determination of genome size in European sunbleak Leucaspius delineatus (Heckel, 1843)
Article Snippet: .. Protein concentration analysis Blood cells and liver fragments isolated from L. delineatus were lysed for 30 min at 4°C with a buffer containing Tris–HCl, pH 7.5 (20 mM, Merck, Germany), sodium chloride (150 mM, Chempur, Poland), Na2 EDTA (1 mM, Sigma–Aldrich, Germany), EGTA (1 mM, Sigma, Germany), 1% Triton X-100 (Calbiochem, USA), sodium pyrophosphate (2.5 mM, POCH, Poland), β-glycerophosphate (1 mM, Laboratory BDH Reagent, GB). .. Then, the samples were mechanically homogenized and total protein content was estimated using a Micro BCA Protein Kit (Thermo Scientific, USA).

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    Avantor sodium pyrophosphate
    Sodium Pyrophosphate, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Avantor
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    93/100 stars
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