sodium pyrophosphate tetrabasic decahydrate  (Millipore)


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  • 95
    Name:
    Sodium pyrophosphate tetrabasic decahydrate
    Description:

    Catalog Number:
    p9146
    Price:
    None
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    Structured Review

    Millipore sodium pyrophosphate tetrabasic decahydrate
    Sodium pyrophosphate tetrabasic decahydrate

    https://www.bioz.com/result/sodium pyrophosphate tetrabasic decahydrate/product/Millipore
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate tetrabasic decahydrate - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    Lysis:

    Article Title: Pubertal hormones mediate sex differences in levels of myelin basic protein in the orbitofrontal cortex of adult rats
    Article Snippet: .. Tissue was homogenized in 20 μl/mg lysis buffer containing 1mM EGTA, 1mM EDTA, 20 mM Tris, 1 mM sodium pyrophosphate tetrabasic decahydrate, 4 mM 4-nitrophenyl phosphate disodium salt hexahydrate, 0.1 μM microcystin, and 1% protease inhibitor cocktail (Sigma-Aldrich). .. Samples were then centrifuged for 15 min at 1000 × g at 4°C, protein concentration of supernatants was determined (Bradford Protein Assay Kit; Pierce, Rockford, IL), and each sample was diluted 1:1 with Laemmli Sample Buffer (Bio- Rad; Hercules, CA), mixed with 350 mM D,L-dithiothreitol, boiled for 5 min, and stored at −80°C.

    Article Title: AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells
    Article Snippet: .. Cells were lysed with lysis buffer (8 M urea [Sigma-Aldrich, U5378], 75 mM NaCl [Sigma-Aldrich, 31,434], 50 mM Tris [Sigma-Aldrich, T1503], pH 8.2, one tablet of protease inhibitors cocktail [Sigma-Aldrich, P834099], 1 mM sodium fluoride [Fluka, 71,519], 1 mM sodium β-glycerophosphate [Cayman Chemical, 13,408–09-8], 1 mM sodium orthovanadate [Sigma-Aldrich, 450,243-50G], 10 mM sodium pyrophosphate [Sigma-Aldrich, S6422-100G], 1 mM phenylmethylsulfonyl fluoride [Sigma-Aldrich, P7626]) and lysates were sonicated 3 times for 60 sec with an output of 15%. ..

    Article Title: Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats
    Article Snippet: .. Tissue was homogenized in 15 μl/mg lysis buffer containing 1mM EGTA, 1mM EDTA, 20 mM Tris, 1 mM sodium pyrophosphate tetrabasic decahydrate, 4 mM 4-nitrophenyl phosphate disodium salt hexahydrate, 0.1 μM microcystin, and 1% protease inhibitor cocktail (Sigma-Aldrich). .. Samples were centrifuged for 15 min at 1000 × g at 4 C, protein concentration of supernatants was determined (Bradford Protein Assay Kit; Pierce, Rockford, IL), and each sample was diluted 1:1 with Laemmli Sample Buffer (Bio-Rad; Hercules, CA) mixed with 350 mM D,L-dithiothreitol, boiled for 5 min, and stored at −80 C.

    Protease Inhibitor:

    Article Title: Pubertal hormones mediate sex differences in levels of myelin basic protein in the orbitofrontal cortex of adult rats
    Article Snippet: .. Tissue was homogenized in 20 μl/mg lysis buffer containing 1mM EGTA, 1mM EDTA, 20 mM Tris, 1 mM sodium pyrophosphate tetrabasic decahydrate, 4 mM 4-nitrophenyl phosphate disodium salt hexahydrate, 0.1 μM microcystin, and 1% protease inhibitor cocktail (Sigma-Aldrich). .. Samples were then centrifuged for 15 min at 1000 × g at 4°C, protein concentration of supernatants was determined (Bradford Protein Assay Kit; Pierce, Rockford, IL), and each sample was diluted 1:1 with Laemmli Sample Buffer (Bio- Rad; Hercules, CA), mixed with 350 mM D,L-dithiothreitol, boiled for 5 min, and stored at −80°C.

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

    Article Title: Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats
    Article Snippet: .. Tissue was homogenized in 15 μl/mg lysis buffer containing 1mM EGTA, 1mM EDTA, 20 mM Tris, 1 mM sodium pyrophosphate tetrabasic decahydrate, 4 mM 4-nitrophenyl phosphate disodium salt hexahydrate, 0.1 μM microcystin, and 1% protease inhibitor cocktail (Sigma-Aldrich). .. Samples were centrifuged for 15 min at 1000 × g at 4 C, protein concentration of supernatants was determined (Bradford Protein Assay Kit; Pierce, Rockford, IL), and each sample was diluted 1:1 with Laemmli Sample Buffer (Bio-Rad; Hercules, CA) mixed with 350 mM D,L-dithiothreitol, boiled for 5 min, and stored at −80 C.

    Transferring:

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

    Size-exclusion Chromatography:

    Article Title: AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells
    Article Snippet: .. Cells were lysed with lysis buffer (8 M urea [Sigma-Aldrich, U5378], 75 mM NaCl [Sigma-Aldrich, 31,434], 50 mM Tris [Sigma-Aldrich, T1503], pH 8.2, one tablet of protease inhibitors cocktail [Sigma-Aldrich, P834099], 1 mM sodium fluoride [Fluka, 71,519], 1 mM sodium β-glycerophosphate [Cayman Chemical, 13,408–09-8], 1 mM sodium orthovanadate [Sigma-Aldrich, 450,243-50G], 10 mM sodium pyrophosphate [Sigma-Aldrich, S6422-100G], 1 mM phenylmethylsulfonyl fluoride [Sigma-Aldrich, P7626]) and lysates were sonicated 3 times for 60 sec with an output of 15%. ..

    Sonication:

    Article Title: AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells
    Article Snippet: .. Cells were lysed with lysis buffer (8 M urea [Sigma-Aldrich, U5378], 75 mM NaCl [Sigma-Aldrich, 31,434], 50 mM Tris [Sigma-Aldrich, T1503], pH 8.2, one tablet of protease inhibitors cocktail [Sigma-Aldrich, P834099], 1 mM sodium fluoride [Fluka, 71,519], 1 mM sodium β-glycerophosphate [Cayman Chemical, 13,408–09-8], 1 mM sodium orthovanadate [Sigma-Aldrich, 450,243-50G], 10 mM sodium pyrophosphate [Sigma-Aldrich, S6422-100G], 1 mM phenylmethylsulfonyl fluoride [Sigma-Aldrich, P7626]) and lysates were sonicated 3 times for 60 sec with an output of 15%. ..

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  • News
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  • 96
    Millipore sodium pyrophosphate tetrabasic
    Sodium Pyrophosphate Tetrabasic, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate tetrabasic/product/Millipore
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate tetrabasic - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    95
    Millipore sodium pyrophosphate
    Sodium Pyrophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Millipore
    Average 95 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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