sodium pyrophosphate nappi  (Millipore)


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  • 99
    Name:
    Tetrasodium Pyrophosphate Decahydrate
    Description:
    Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost effective alternative to the preparation of in house working standards
    Catalog Number:
    phr2205
    Price:
    None
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    Structured Review

    Millipore sodium pyrophosphate nappi
    Tetrasodium Pyrophosphate Decahydrate
    Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost effective alternative to the preparation of in house working standards
    https://www.bioz.com/result/sodium pyrophosphate nappi/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate nappi - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell–cell contacts
    Article Snippet: .. Xenopus embryo protein lysate preparation and Western blot Xenopus whole embryos were solubilized in lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM phenylmethylsulfonofluoride, 1% mammalian protease inhibitor cocktail [Sigma-Aldrich, P2714], 0.2 mM H2 O2, 3 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 10 mM sodium fluoride, sodium β-glycerophosphate [10 mg/ml], 1% Triton X-100 [Sigma-Aldrich, T-9284], or 1% Tergitol type NP-40 [Spectrum Biosciences, T1279]). .. Protein samples were prepared in 2× Laemmli buffer supplemented with 5% β-mercaptoethanol, incubated on a 95°C heat block for 5 min, and loaded onto 12% SDS–PAGE gels.

    Lysis:

    Article Title: 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell–cell contacts
    Article Snippet: .. Xenopus embryo protein lysate preparation and Western blot Xenopus whole embryos were solubilized in lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM phenylmethylsulfonofluoride, 1% mammalian protease inhibitor cocktail [Sigma-Aldrich, P2714], 0.2 mM H2 O2, 3 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 10 mM sodium fluoride, sodium β-glycerophosphate [10 mg/ml], 1% Triton X-100 [Sigma-Aldrich, T-9284], or 1% Tergitol type NP-40 [Spectrum Biosciences, T1279]). .. Protein samples were prepared in 2× Laemmli buffer supplemented with 5% β-mercaptoethanol, incubated on a 95°C heat block for 5 min, and loaded onto 12% SDS–PAGE gels.

    Article Title: Degradation of PHLPP2 by KCTD17, via a Glucagon-dependent Pathway, Promotes Hepatic Steatosis
    Article Snippet: .. To examine PHLPP2 interacting proteins, liver lysates were extracted in less stringent lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1mM EDTA, 10% glycerol, 1% NP-40, 10 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 50 mM sodium fluoride, 1.5 mM sodium orthovanadate, and protease inhibitor cocktail], immunoprecipitated with anti-Flag M2 agarose, and bound polypeptides eluted with FLAG peptide (Sigma). .. Aliquots of each sample were loaded onto an Acclaim PepMap 100 precolumn (75 μm × 2 cm, C18, 3 μm, 100Å, Thermo Scientific) in-line with an EASY-Spray, PepMap column (75 μm × 50 cm, C18, 2 μm, 100Å Thermo Scientific) using the autosampler of an EASY-nLC 1000 (Thermo Scientific).

    Article Title: Overexpression of ryanodine receptor type 1 enhances mitochondrial fragmentation and Ca2+-induced ATP production in cardiac H9c2 myoblasts
    Article Snippet: .. The pellet (mitochondrial protein fraction) was resuspended in lysis buffer (Cell Signaling Technology) containing 20 mM Tris·HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.2% SDS, 2.5 mM sodium pyrophosphate, 1 μM β-glycerophosphate, 1 mM NaVO4 , 50 mM NaF, 1 mM PMSF, and 1% protease inhibitor cocktail (Sigma-Aldrich). ..

    Article Title: Ecdysteroids Elicit a Rapid Ca2+ Flux Leading to Akt Activation and Increased Protein Synthesis in Skeletal Muscle Cells
    Article Snippet: .. Lysis buffer consisted of 20 mM Tris, pH 7.4, 30 mM NaCl, 3.5 mM EDTA, 1 mM EGTA, 1.0% Triton X-100, 2.5 mM sodium pyrophosphate, 4 mM sodium fluoride, 1 mM sodium vanadate, 1mM phenylmethylsulfonyl fluoride and Protease Inhibitor Cocktail (Sigma) consisting of 2.0 μg/ml leupeptin and 2.0 μg/ml aprotinin. .. Two wells per treatment were pooled and whole cell lysates were sonicated for 15 seconds, boiled for 5 min, and centrifuged at 16,000 g for 5 min at 4 C. Protein content of the supernatant was quantified using a Nanodrop 1000 spectrophotometer according to manufacturer instructions (Thermo Scientific).

    Article Title: PIM1 Phosphorylates and Negatively Regulates ASK1-mediated Apoptosis
    Article Snippet: .. Cells were harvested and lysed in lysis buffer (50mM Tris-HCL(pH7.5), 1%(v/v) NP-40, 50 mM NaCl, 1 mM EDTA, 1 mM EGTA, 10 mM sodium B-glycerophosphate, 5 mM activated sodium orthovanadate, 5 mM sodium pyrophosphate, 50 mM sodium fluoride, and protease inhibitor cocktail set I (Calbiochem)) by incubating on ice for 30 minutes. ..

    Article Title: PPAR? agonist GW501516 prevents uncoupling of endothelial nitric oxide synthase in cerebral microvessels of hph-1 mice
    Article Snippet: .. Cerebral microvessels were homogenized in lysis buffer containing [50 mmol/L NaCl, 50 mmol/L NaF, 50 mmol/L sodium pyrophosphate, 5 mmol/L EDTA, 5 mmol/L EGTA, 0.1 mmol/L Na3 VO4 , 1% Triton X-100, 10 mmol/L HEPES, pH 7.4, and protease inhibitor cocktail (Sigma)]. ..

    Protease Inhibitor:

    Article Title: 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell–cell contacts
    Article Snippet: .. Xenopus embryo protein lysate preparation and Western blot Xenopus whole embryos were solubilized in lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM phenylmethylsulfonofluoride, 1% mammalian protease inhibitor cocktail [Sigma-Aldrich, P2714], 0.2 mM H2 O2, 3 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 10 mM sodium fluoride, sodium β-glycerophosphate [10 mg/ml], 1% Triton X-100 [Sigma-Aldrich, T-9284], or 1% Tergitol type NP-40 [Spectrum Biosciences, T1279]). .. Protein samples were prepared in 2× Laemmli buffer supplemented with 5% β-mercaptoethanol, incubated on a 95°C heat block for 5 min, and loaded onto 12% SDS–PAGE gels.

    Article Title: Degradation of PHLPP2 by KCTD17, via a Glucagon-dependent Pathway, Promotes Hepatic Steatosis
    Article Snippet: .. To examine PHLPP2 interacting proteins, liver lysates were extracted in less stringent lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1mM EDTA, 10% glycerol, 1% NP-40, 10 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 50 mM sodium fluoride, 1.5 mM sodium orthovanadate, and protease inhibitor cocktail], immunoprecipitated with anti-Flag M2 agarose, and bound polypeptides eluted with FLAG peptide (Sigma). .. Aliquots of each sample were loaded onto an Acclaim PepMap 100 precolumn (75 μm × 2 cm, C18, 3 μm, 100Å, Thermo Scientific) in-line with an EASY-Spray, PepMap column (75 μm × 50 cm, C18, 2 μm, 100Å Thermo Scientific) using the autosampler of an EASY-nLC 1000 (Thermo Scientific).

    Article Title: Overexpression of ryanodine receptor type 1 enhances mitochondrial fragmentation and Ca2+-induced ATP production in cardiac H9c2 myoblasts
    Article Snippet: .. The pellet (mitochondrial protein fraction) was resuspended in lysis buffer (Cell Signaling Technology) containing 20 mM Tris·HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.2% SDS, 2.5 mM sodium pyrophosphate, 1 μM β-glycerophosphate, 1 mM NaVO4 , 50 mM NaF, 1 mM PMSF, and 1% protease inhibitor cocktail (Sigma-Aldrich). ..

    Article Title: Ecdysteroids Elicit a Rapid Ca2+ Flux Leading to Akt Activation and Increased Protein Synthesis in Skeletal Muscle Cells
    Article Snippet: .. Lysis buffer consisted of 20 mM Tris, pH 7.4, 30 mM NaCl, 3.5 mM EDTA, 1 mM EGTA, 1.0% Triton X-100, 2.5 mM sodium pyrophosphate, 4 mM sodium fluoride, 1 mM sodium vanadate, 1mM phenylmethylsulfonyl fluoride and Protease Inhibitor Cocktail (Sigma) consisting of 2.0 μg/ml leupeptin and 2.0 μg/ml aprotinin. .. Two wells per treatment were pooled and whole cell lysates were sonicated for 15 seconds, boiled for 5 min, and centrifuged at 16,000 g for 5 min at 4 C. Protein content of the supernatant was quantified using a Nanodrop 1000 spectrophotometer according to manufacturer instructions (Thermo Scientific).

    Article Title: PIM1 Phosphorylates and Negatively Regulates ASK1-mediated Apoptosis
    Article Snippet: .. Cells were harvested and lysed in lysis buffer (50mM Tris-HCL(pH7.5), 1%(v/v) NP-40, 50 mM NaCl, 1 mM EDTA, 1 mM EGTA, 10 mM sodium B-glycerophosphate, 5 mM activated sodium orthovanadate, 5 mM sodium pyrophosphate, 50 mM sodium fluoride, and protease inhibitor cocktail set I (Calbiochem)) by incubating on ice for 30 minutes. ..

    Article Title: PPAR? agonist GW501516 prevents uncoupling of endothelial nitric oxide synthase in cerebral microvessels of hph-1 mice
    Article Snippet: .. Cerebral microvessels were homogenized in lysis buffer containing [50 mmol/L NaCl, 50 mmol/L NaF, 50 mmol/L sodium pyrophosphate, 5 mmol/L EDTA, 5 mmol/L EGTA, 0.1 mmol/L Na3 VO4 , 1% Triton X-100, 10 mmol/L HEPES, pH 7.4, and protease inhibitor cocktail (Sigma)]. ..

    Immunoprecipitation:

    Article Title: Degradation of PHLPP2 by KCTD17, via a Glucagon-dependent Pathway, Promotes Hepatic Steatosis
    Article Snippet: .. To examine PHLPP2 interacting proteins, liver lysates were extracted in less stringent lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1mM EDTA, 10% glycerol, 1% NP-40, 10 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 50 mM sodium fluoride, 1.5 mM sodium orthovanadate, and protease inhibitor cocktail], immunoprecipitated with anti-Flag M2 agarose, and bound polypeptides eluted with FLAG peptide (Sigma). .. Aliquots of each sample were loaded onto an Acclaim PepMap 100 precolumn (75 μm × 2 cm, C18, 3 μm, 100Å, Thermo Scientific) in-line with an EASY-Spray, PepMap column (75 μm × 50 cm, C18, 2 μm, 100Å Thermo Scientific) using the autosampler of an EASY-nLC 1000 (Thermo Scientific).

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  • 91
    Millipore nappi
    Nappi, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nappi/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nappi - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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